Fe-N-C single-atom nanozyme for ultrasensitive, on-site and multiplex detection of mycotoxins using lateral flow immunoassay.

Sensitive, on-site and multiple detection of mycotoxins is a vital early-warning tool to minimize food losses and protect human health and the environment. Although paper-based lateral flow immunoassay (LFIA) has been extensively applied in mycotoxins monitoring, low-cost, portable, ultrasensitive and quantitative detection is still a formidable challenge. Herein, a series of Fe-N-C single-atom nanozymes (SAzymes) were synthesized and systematic characterized.

A versatile nanozyme integrated colorimetric and photothermal lateral flow immunoassay for highly sensitive and reliable Aspergillus flavus detection.

Visual lateral flow immunoassays (LFA) have been recognized as the attractive point-of-care testing (POCT) for bioanalysis; however, they have been constrained by insufficient sensitivity and limited reliability. Herein, combining the catalytic sites of Cu nanoparticles with an inherent photothermal polydopamine (PDA) scaffold via a one-step process, a compact Cu-anchored PDA (PCu) was engineered as the efficient signal element for the multimodal LFA (mLFA). The robust PCu with peroxidase-mimics and photothermal properties, could simultaneously provide triple signal readouts for colorimetric, amplified colorimetric and photothermal detection toward Aspergillus flavus (A.

Nanozyme-strip based on MnO nanosheets as a catalytic label for multi-scale detection of aflatoxin B with an ultrabroad working range.

Lateral flow immunoassay (LFIA) is the most effective real-time detection method for aflatoxin B (AFB). Here, we constructed a nanozyme-strip based on MnO nanosheets (MnO NSs) as a catalytic label for detection of AFB. By taking advantage of the MnO-TMB catalytic amplified system, the new test achieves rapid detection with high sensitivity and ultrawide range.

A direct competitive nanozyme-linked immunosorbent assay based on MnO nanosheets as a catalytic label for the determination of fumonisin B.

A direct competitive nanozyme-linked immunosorbent assay (dcNLISA) based on MnO nanosheets (MnO NSs) as a nanozyme label was developed for the highly sensitive determination of fumonisin B (FB). MnO NS-labeled fumonisin B-bovine serum albumin was easily synthesized as a competing antigen for the dcNLISA. And color changes derived from the MnO-3,3',5,5'-tetramethylbenzidine (TMB) system were exploited as the output signals of the dcNLISA.

Adsorptive removal of aflatoxin B from vegetable oils via novel adsorbents derived from a metal-organic framework.

Vegetable oils are essential daily diet, but they are simply contaminated with aflatoxin B (AFB), a serious toxic compound to human health. Adsorption method due to the easy operation, high efficiency and low costing is set to become a main detoxification technique for AFB. Unfortunately, previous reported adsorbents were rarely used for detoxification in food industry since they cannot meet the criteria of large-scale production of edible oils.