Visualizing spatiotemporal pattern of vascularization by SWIR fluorescence imaging in a mouse model of perforator flap transplantation.

Vascularization as a spatiotemporally interlaced process involving angiogenesis and vascular remodeling, has seldom been investigated comprehensively regarding the interrelationship of the two intertwining but sequential processes. Here, a shortwave infrared (SWIR) fluorescence imaging strategy based on quantum dots (QDs) was designed to dynamically visualize vascularization in vivo and in situ in a perforator transplantation mouse model. The vascularization process could be directly perceived from the established flap model with an optimal observation window at 10 min post-injection.

Single-Dose Radial Extracorporeal Shock Wave Therapy Modulates Inflammation During Meniscal Tear Healing in the Avascular Zone.

Background: Extracorporeal shock wave therapy (ESWT) promotes tissue healing by modulating inflammation, which has implications for meniscal tear healing in the avascular zone.

Purpose: To evaluate the effects of a single dose of radial ESWT on the healing process and inflammation of the meniscus and knee joints after meniscal tears in the avascular zone.

Study Design: Controlled laboratory study.

Three-Dimensional Imaging and Quantitative Analysis of Blood Vessel Distribution in The Meniscus of Transgenic Mouse after Tissue Clearing.

Objective: Blood supply to the meniscus determines its recovery and is a reference for treatment planning. This study aimed to apply tissue clearing and three-dimensional (3D) imaging in exploring the quantitative distribution of blood vessels in the mouse meniscus.

Materials And Methods: In this experimental study, tissue clearing was performed to treat the bilateral knee joints of transgenic mice with fluorescent vascular endothelial cells.