Development of Loop-Mediated Isothermal Amplification Method for Rapid and Sensitive Identification of (Diptera: Stratiomyidae).

The black soldier fly (BSF) is well known for its ability to biologically convert organic waste into insect biomass, including protein and oil, which can be utilised as animal feed. Since raw BSF products, such as BSF powder, are difficult to differentiate from other biological raw materials, therefore new analytical approaches are required. In this study, we have developed a new and fast method based on loop-mediated isothermal AMPlification (LAMP) reaction that can diagnose black soldier fly larvae and BSF byproducts with high accuracy, specificity and sensitivity.

Vermicompost Amendments Disrupt Feeding Behavior of Kuwayama and Boost Activities of Salicylic Acid and Jasmonic Acid Pathway-Related Enzymes in Citrus.

Plants grown with vermicompost amendments are known to be harmful to sap-sucking insects, but the underlying mechanism remains to be determined. Here we investigated the feeding behavior of Kuwayama on (L.) Burm.

Assessing the Bacterial Communities Composition from Differently Treated Agarwood via 16S rRNA Gene Metabarcoding.

Agarwood () is one of the most important resin-containing plants used to produce agar around the world and it is a precious herbal medicine usually combined with other herbs. In this study, we used the Illumina sequencing technique to explore the agarwood bacterial community structure from four different incense formations of agarwood, including healthy agarwood, drilling agarwood, liquid fermentation agarwood, and insect attack agarwood. Our results showed that 20 samples of three different incense-formation methods of agarwood and healthy agarwood acquired 1,792,706 high-quality sequences.

Plant jasmonic acid mediated contrasting effects of two citrus aphid species on Diaphorina citri Kuwayama.

Background: Herbivores may influence each other directly and through plant mediated inter-specific interactions. The Asian citrus psyllid (Diaphorina citri Kuwayama) and citrus aphids are key pests that can co-exist on citrus, but their plant-mediated interaction between them is unknown. Here we investigated plant-mediated effect of two citrus aphid species, the polyphagous Aphis spiraecola Patch and the oligophagous Aphis (Toxoptera) citricidus (Kirkaldy) on the feeding behavior and reproduction of Diaphorina citri, and explored the underlying mechanisms.

Identification and Expression Profiling of Chemosensory Genes in via a Transcriptomic Analysis.

The black soldier fly, , is a cosmopolitan insect of the family Stratiomyidae (Diptera). Chemosensory genes encode proteins involved directly in the detection of odorants. In this study, we sequenced the antennal transcriptome of adults to identify chemosensory genes.

Toxicities of destruxins against Bemisia tabaci and its natural enemy, Serangium japonicum.

The bioactivities of destruxins against whitefly, Bemisia tabaci and its natural enemy, ladybird beetle Serangium japonicum were evaluated. Destruxins A and B (DA and DB) showed insignificant ovicidal, oviposition deterrent and systemic insecticidal activities to B. tabaci; however, DA and DB had certain contact virulence to its nymphs.