CD34+ cell dose and CMV viremia after haploidentical hematopoietic stem cell transplantation: a retrospective study.

Background: Patients with hematologic malignancies who undergo Haplo-HSCT (Haploidentical hematopoietic stem cell transplantation) are at a significantly higher risk for CMV (Cytomegalovirus) infection than individuals with normal immune function. This increased susceptibility to CMV is a major contributor to the morbidity and mortality seen in patients following hematopoietic stem cell transplantation.

Methods: This study involved a retrospective analysis of 113 patients who underwent allogeneic hematopoietic stem cell transplantation for malignant hematological diseases.

TET2 mutations contribute to adverse prognosis in acute myeloid leukemia (AML): results from a comprehensive analysis of 502 AML cases and the Beat AML public database.

Despite the high incidence of tet methylcytosine dioxygenase 2 (TET2) mutations in acute myeloid leukemia (AML), the prognostic implications of these mutations in three AML risk groups based on the 2022 ELN AML risk classification are still unclear. A total of 502 consecutive de novo AML patients who had next-generation sequencing data available between March 2011 and July 2021 at the Peking University Institute of Hematology were enrolled in this study. Univariate and multivariate Cox regression analyses were performed to explore the prognostic impact of TET2 mutations in the above cohort and the Beat AML cohort.

Prognostic impact of FLT3-ITD mutation on NPM1 acute myeloid leukaemia patients and related molecular mechanisms.

The prognosis of acute myeloid leukaemia (AML) patients carrying NPM1 mutations is significantly worse when accompanied by FLT3-ITD mutations. However, accurate quantitative detection of FLT3-ITD mutations remains challenging. To identify a novel biomarker in NPM1 FLT3-ITD AML patients for more accurate stratification, we analysed the differential gene expression between the NPM1 FLT3-ITD and NPM1 FLT3-ITD groups in five public AML datasets and identified a biomarker by taking the intersection of differentially expressed genes.

The adverse impact of ecotropic viral integration site-1 (EVI1) overexpression on the prognosis of acute myeloid leukemia with KMT2A gene rearrangement in different risk stratification subtypes.

Introduction: AML patients with KMT2A-MLLT3 and other 11q23 abnormalities belong to the intermediate and high-risk level groups, respectively. Whether the poor prognostic value of Ecotropic Viral Integration site-1 (EVI1) overexpression suits either the subtypes of KMT2A-MLLT3 or Non-KMT2A-MLLT3 AML patients (intermediate and high risk group) needs to be further investigated.

Methods: We retrospectively analyzed the clinical characteristics of 166 KMT2A-r and KMT2A-PTD AML patients.

Significance of WT1 and multiparameter flow cytometry assessment in patients with chronic myelomonocytic leukemia receiving allogeneic hematopoietic stem cell transplantation.

Introduction: The objective of this study was to investigate the clinical significance of minimal residual disease (MRD) monitoring through Wilms tumor 1 (WT1) gene expression and multicolor flow cytometry (FCM) in patients with chronic myelomonocytic leukemia (CMML) after allogeneic hematopoietic stem cell transplantation (allo-HSCT).

Methods: For this purpose, WT1 gene expression and the CMML-related abnormal immunophenotype were examined using real-time quantitative polymerase chain reaction and FCM, respectively.

Results: In total, 59 patients with CMML who underwent allo-HSCT were enrolled in this study.

Autophagy and Age-Related Eye Diseases.

Background: Autophagy is a catabolic process that depends on the lysosome. It is usually used to maintain cellular homeostasis, survival and development by degrading abnormal substances and dysfunctional organelles, especially when the cell is exposed to starvation or other stresses. Increasing studies have reported that autophagy is associated with various eye diseases, of which aging is one of the important factors.