NiCo LDH nanozymes with selective antibacterial activity against Gram-negative bacteria for wound healing.

Bacterial infections have been a major threat to human health. Especially, Gram-negative (G) bacterial infections have been an increasing problem worldwide. The overuse of antibiotics leads to an emergence of drug resistance, and thus the development of novel antimicrobial agents is important, particularly against G bacteria.

Alkaline-promoted regulation of the peroxidase-like activity of Ni/Co LDHs and development bioassays.

Nanozymes' activities could be regulated by a simple and effective pH change in an in situ manner. In this work, for the first time, the peroxidase-like activity of Ni/Co layered double hydroxides (LDHs) was regulated via the alkaline-promoted reaction of fluorogenic substrate homovanillic acid and HO, and a promising tool for pH sensing was developed over the pH range of 8.3-9.

Evaluation of fluorogenic substrates for Ni/Co LDHs peroxidase mimic and application for determination of inhibitory effects of antioxidant.

Nanomaterial-based peroxidase-mimetics are an emerging research field that promises to produce alternatives to horseradish peroxidase for a variety of applications. Generally, some peroxidase-mimetics substrates are used in acidic condition (pH ≤ 7). Then, it is necessary to screen some peroxidase-mimetics substrates suitable for basic condition because that some peroxidase-mimetics leached ions in acidic solution.

One-step analysis of glucose and acetylcholine in water based on the intrinsic peroxidase-like activity of Ni/Co LDHs microspheres.

In the present study, a simple strategy was developed for Ni/Co layered double hydroxides (LDHs) as a substitute for natural peroxidase. The obtained Ni/Co LDHs exhibited ease of preparation, low-cost, and water-solubility; importantly, this material showed high catalytic activity in neutral pH solutions (phosphate buffer, Tris-HCl buffer, and even water). Benefitting from Ni/Co LDHs having a similar pH and temperature with specificity oxidase, such as glucose oxidase, choline oxidase, acetylcholinesterase, etc.