Effects of ovarian endometrioma aspiration on in vitro fertilization-intracytoplasmic sperm injection and embryo transfer outcomes: a systematic review and meta-analysis.

Purpose: To evaluate the effect of ovarian endometrioma aspiration on IVF/ICSI outcomes.

Methods: The PubMed, EMBASE, Web of Science, China National Knowledge Infrastructure, and Wanfang databases were searched to identify studies related to the treatment of endometrioma up to October 1, 2020, and the data of 1207 patients from 10 studies were analyzed using STATA.

Results: The 10 studies in our analysis included 7 comparing aspiration and surgery and 6 comparing aspiration with no intervention.

Association of Myometrial Invasion With Lymphovascular Space Invasion, Lymph Node Metastasis, Recurrence, and Overall Survival in Endometrial Cancer: A Meta-Analysis of 79 Studies With 68,870 Patients.

Background: Myometrial invasion has been demonstrated to correlate to clinicopathological characteristics and prognosis in endometrial cancer. However, not all the studies have the consistent results and no meta-analysis has investigated the association of myometrial invasion with lymphovascular space invasion (LVSI), lymph node metastasis (LNM), recurrence, and overall survival (OS). Therefore, a meta-analysis was performed to evaluate the relationship between myometrial invasion and clinicopathological characteristics or overall survival in endometrial cancer.

An asymmetric oxidative cyclization/Mannich-type addition cascade reaction for direct access to chiral pyrrolidin-3-ones.

An efficient gold and chiral phosphoric acid cooperatively catalyzed enantioselective oxidative cyclization/Mannich-type addition reaction of homopropargyl amides with nitrones has been developed, which provides chiral pyrrolidin-3-ones in high yields with excellent enantioselectivities under mild conditions. This reaction employed stable and readily available alkynes as non-diazo carbene precursors, which provides a 100% atom economy method with high bond formation efficiency.

Gold-based immunochromatographic assay strip for the detection of quinclorac in foods.

In this study, a highly specific and sensitive monoclonal antibody (mAb) against quinclorac (Qui) was prepared. Based on the selected mAb, 2G3, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and an immunochromatographic strip assay were established for the detection of Qui in actual samples. The 50%-inhibitory concentration of mAb 2G3 against Qui was 48.

RON Mediates Tumor-Promoting Effects in Endometrial Adenocarcinoma.

Endometrial adenocarcinoma is one of the most prevalent female reproductive tract cancers in the world, and the development of effective treatment is still the main goal of its current research. Epithelial-mesenchymal transition (EMT) plays a significant part in the occurrence and development of epithelial carcinoma, including endometrial adenocarcinoma. Recepteur d'origine nantais (RON) induces EMT and promotes proliferation, migration, and invasion in various epithelial-derived cancers, but its role in endometrial adenocarcinoma is still poorly studied.

CRISPR/Cas9-mediated genome editing directed by a 5S rRNA-tRNA hybrid promoter in the thermophilic filamentous fungus Humicola insolens.

Background: Humicola insolens is a filamentous fungus with high potential of producing neutral and heat- and alkali-resistant cellulase. However, the genetic engineering tools, particularly the genome-editing tool, are scarce, hindering the study of cellulase expression regulation in this organism.

Results: Herein, a CRISPR/Cas9 genome-editing system was established in H.

NLRP3 Inflammasome Activation of Mast Cells by Estrogen the Nuclear-Initiated Signaling Pathway Contributes to the Development of Endometriosis.

Endometriosis is an estrogen-dependent gynecological disease. The pathogenesis of endometriosis remains controversial, although it is generally accepted that the inflammatory immune response plays a crucial role in this process. Mast cells (MCs) are multifunctional innate immune cells that accumulate in endometriotic lesions.

Effectiveness of 23-Valent Pneumococcal Polysaccharide Vaccine Against Pneumococcal Diseases Among the Elderly Aged 60 Years or Older: A Matched Test Negative Case-Control Study in Shanghai, China.

infection among adults, especially in adults over 60 years old in China results in a large number of hospitalizations and a substantial financial burden. This study assessed the vaccine effectiveness (VE) of 23-valent pneumococcal polysaccharide vaccine (PPV23) against pneumococcal diseases among the elderly aged 60 years or older in Shanghai, China. We conducted a test-negative case-control study among the elderly aged 60 years or older who sought care at hospitals in 13 districts of Shanghai from September 14, 2013 to August 31, 2019.

β-galactosidase GALA from with high transgalactosylation activity.

β-galactosidase catalyzes lactose hydrolysis and transfers reactions to produce prebiotics such as galacto-oligosaccharides (GOS) with potential applications in the food industry and pharmaceuticals. However, there is still a need for improved transgalactosylation activity of β-galactosidases and reaction conditions of GOS production in order to maximize GOS output and reduce production costs. In this study, a β-galactosidase gene, , from was expressed in , which not only hydrolyzed lactose but also had strong transgalactosylation activity to produce GOS.

Rapid determination of trace Cu by an in-syringe membrane SPE and membrane solid-phase spectral technique.

A new in-syringe membrane SPE and solid-phase visible spectral method was proposed for the rapid extraction and visible spectral determination of trace Cu. The chelation and membrane SPE can be accomplished in a syringe. The yellow Cu(DDTC) complex was separated using a polyethersulfone membrane from the sample solution.

Simultaneous detection of phenacetin and paracetamol using ELISA and a gold nanoparticle-based immunochromatographic test strip.

We have developed a sensitive and rapid gold nanoparticle-based immunochromatographic strip (GNP-ICS) for the detection of phenacetin (PNCT) and paracetamol (PAP) using an anti-PNCT monoclonal antibody (mAb). The sensitive anti-PNCT mAb (2D6) had a half maximal inhibitory concentration (IC) and limit of detection (LOD) of 3.51 and 0.

Structural Modulation on NiCo S Nanoarray by N Doping to Enhance 2e-ORR Selectivity for Photothermal AOPs and Zn-O Batteries*.

As a H O generator, a 2e oxygen reduction reaction active electrocatalyst plays an important role in the advanced oxidation process to degrade organic pollutants in sewage. To enhance the tendency of NiCo S towards the 2e reduction reaction, N atoms are doped in its structure and replace S . The result implies that this weakens the interaction between NiCo S and OOH*, suppresses O-O bond breaking and enhances H O selectivity.

An immunochromatographic sensor for ultrasensitive and direct detection of histamine in fish.

To ensure food quality and prevent histamine (HA) toxicity, a rapid and direct method of detecting HA is required. In this work, we prepared a monoclonal antibody (mAb) against HA using a hapten produced by the introduction of a phenyl-containing linker. The novel mAb exhibited high sensitivity against HA as determined by ELISA, with a half-maximal inhibitory concentration of 21.

Application of multi-layer denoising based on ensemble empirical mode decomposition in extraction of fault feature of rotating machinery.

Aiming at the problem that the weak features of non-stationary vibration signals are difficult to extract under strong background noise, a multi-layer noise reduction method based on ensemble empirical mode decomposition (EEMD) is proposed. First, the original vibration signal is decomposed by EEMD, and the main intrinsic modal components (IMF) are selected using comprehensive evaluation indicators; the second layer of filtering uses wavelet threshold denoising (WTD) to process the main IMF components. Finally, the virtual noise channel is introduced, and FastICA is used to de-noise and unmix the IMF components processed by the WTD.

Deciphering and predicting anammox-based nitrogen removal process under oxytetracycline stress via kinetic modeling and machine learning based on big data analysis.

Anaerobic ammonium oxidation (anammox) is an advanced nitrogen removal process that is widely used in the nitrogen removal of various antibiotic containing wastewaters due to its high efficiency and energy saving characteristics. However, as a widely used antibiotic, the inhibitory effect of oxytetracycline (OTC) on anammox is unclear. In this study, the effect of OTC on the anammox-based nitrogen removal process was revealed by kinetic model and machine learning models.

Ultrasensitive immunochromatographic strip assay for the detection of diminazene.

We prepared monoclonal antibodies (mAbs) against diminazene and used them in the development of a gold nanoparticle-based lateral-flow test (GNT) strip and indirect competitive enzyme-linked immunosorbent assay for the detection of diminazene in beef and beef liver samples. MAbs, which belong to the IgG2a subclass, had a half maximal inhibitory concentration of 0.04 ng mL.

Effectiveness of 13-valent pneumococcal conjugate vaccine against community acquired pneumonia among children in China, an observational cohort study.

Background: In China, 13-valent pneumococcal conjugate vaccine (PCV13) has been available since 2017, but only via the private market with low uptake rate. We assessed the direct effectiveness of PCV13 against community acquired pneumonia (CAP) associated with PCV13 serotype carriage (VT-CAP).

Methods: We conducted an observational cohort study of children born during 12-Dec-2016 to 30-Nov-2018 identified in the Suzhou Centers for Disease Control vaccine registry database, and who had at least one inpatient or outpatient record at the Suzhou University Affiliated Children's hospital (SCH) health-information-system (HIS) database.

Pediatric oncology clinician communication about sexual health with adolescents and young adults: A report from the children's oncology group.

Background: Sexual health (SH) is an important concern for adolescents and young adults (AYAs). This study determined current SH communication practices, barriers, and additional resources needed among pediatric oncology clinicians who treat AYAs.

Methods: A cross-sectional survey was developed by the Children's Oncology Group (COG) AYA Committee and sent to pediatric oncologists (n = 1,987; 85.

Cell subtypes and immune dysfunction in peritoneal fluid of endometriosis revealed by single-cell RNA-sequencing.

Background: Endometriosis is a refractory and recurrent disease and it affects nearly 10% of reproductive-aged women and 40% of infertile patients. The commonly accepted theory for endometriosis is retrograde menstruation where endometrial tissues invade into peritoneal cavity and fail to be cleared due to immune dysfunction. Therefore, the comprehensive understanding of immunologic microenvironment of peritoneal cavity deserves further investigation for the previous studies mainly focus on one or several immune cells.

A gold-based strip sensor for the detection of benzo[]pyrene in edible oils.

This report describes the development of a sensitive and broadly specific indirect competitive enzyme-linked immunosorbent assay (icELISA) and a gold nanoparticle-based immunochromatographic strip (GNP-ICS) assay for the detection of benzo[a]pyrene (B[a]P), using an anti-B[a]P monoclonal antibody (mAb). A broad-specific anti-B[a]P mAb (4E8) was raised from two types of haptens, with half maximal inhibitory concentrations and limits of detection (LOD) values of 2.51 and 0.

Rapamycin Activates Mitophagy and Alleviates Cognitive and Synaptic Plasticity Deficits in a Mouse Model of Alzheimer's Disease.

Alzheimer's disease (AD) is a chronic neurodegenerative disease, which is characterized by cognitive and synaptic plasticity damage. Rapamycin is an activator of autophagy/mitophagy, which plays an important role in identifying and degrading damaged mitochondria. The aim of this study was to investigate the effect of rapamycin on cognitive and synaptic plasticity defects induced by AD, and further explore if the underlying mechanism was associated with mitophagy.

Genetic transformation system for Bacillus velezensis NSZ-YBGJ001 and curing of the endogenous plasmid pBV01.

Objectives: To construct a genetic transformation system for Bacillus velezensis NSZ-YBGJ001 and identify the origin element in an endogenous plasmidpBV01 for curing pBV01 by plasmid incompatibility.

Results: A plasmid pUBC01 was constructed, and then an electrotransformation system for B. velezensis NSZ-YBGJ001 was developed, which reached ~ 1000 transformants per microgram of pUBC01 DNA.

Potential Environmental Health Risk Analysis of Neonicotinoids and a Synergist.

The extensive use of neonicotinoid pesticides has led to their widespread presence in the environment, resulting in considerable safety risks to the ecosystem and human health. In this study, we investigated the biotransformation behavior of a cocktail of multiple neonicotinoids and piperonyl butoxide (PBO) synergist and their potential environmental health risk. It was found that neonicotinoids with a cyano group, such as acetamiprid and thiacloprid, tended to accumulate in liver and spleen tissues, while others with nitro groups (imidacloprid, thiamethoxam, clothianidin, dinotefuran, and nitenpyram) were mostly excreted in urine.

A fluorescence based immunochromatographic sensor for monitoring chlorpheniramine and its comparison with a gold nanoparticle-based lateral-flow strip.

Chlorpheniramine (CPM) is an illegal additive found in herbal teas and health foods, and its excessive intake can cause health problems. In this study, a CPM monoclonal antibody (mAb) was developed based on a new type of hapten. The mAb was found to belong to the IgG2b subclass and showed high sensitivity and specificity when used in ELISA, with a half-maximal inhibitory concentration (IC) of 0.