Direct competitive immunosorbent assay for detection of MEHP in human urine.

Di-(2-ethylhexyl) phthalate (DEHP) is the most commonly used plasticizer for flexible polyvinyl chloride (PVC), which is also known as one of the environmental endocrine disruptors with the reproductive, developmental and embryonic toxicity after entering human body. Mono-2-ethylhexyl phthalate (MEHP) is one of the most complicate metabolites from DEHP in vivo and responsible for many toxic effects of DEHP. In order to evaluate human exposure to DEHP, a direct competitive enzyme-linked immunosorbent (dcELISA) based on monoclonal antibody (mAb) was developed to detect MEHP.

[Resolution of the epinephrine enantiomers derivatized with fluorescent chiral reagent by reversed-phase high performance liquid chromatography].

A new method was developed to separate epinephrine enantiomers derivatized with fluorescent chiral reagent, R-(-)/S-(+)-4-(N, N-dimethylaminosulfonyl)-7-(3-iso-thiocyanatopyrrolidino)-2, 1, 3-benzoxadiazole (DBD-PyNCS), by reversed-phase high performance liquid chromatography, on a Diamonsil C18 column(150 mm x 4.6 mm i.d.