Publications by authors named "Xin-zhi Zhang"
Silencing of SIAH1 in SH-SY5Y affects α-synuclein degradation pathway.
Int J Clin Exp Pathol
October 2016
Seven in absentia homolog (SIAH) is a ubiquitin ligase that monoubiquitinates α-synuclein. Lewy bodies are characteristically rich in monoubiquitinated α-synuclein. We aimed to determine the effect of siRNA-SIAH1 on α-synuclein autophagy and UPS degradation in SH-SY5Y.
View Article and Find Full Text PDFIn this study, we investigated the effect of an antibody against E3 ubiquitin ligase seven in absentia homolog 1 (SIAH-1) in PC12 cells. 1-Methyl-4-phenylpyridinium (MPP(+)) treatment increased α-synuclein, E1 and SIAH-1 protein levels in PC12 cells, and it reduced cell viability; however, there was no significant change in light chain 3 expression. Treatment with an SIAH-1 antibody decreased mRNA expression levels of α-synuclein, light chain 3 and SIAH-1, but increased E1 mRNA expression.
View Article and Find Full Text PDFObjective: To determine the mechanisms by which kangxianling (KXL) treats renal interstitial fibrosis using a customized gene chip.
Methods: Twelve out of 18 specific pathogen-free sprague dawley (SPF SD) rats underwent a unilateral ureteral occlusion. These rats were then randomly assigned into either the model unilateral ureteral obstruction (UUO) or kangxianling (KXL) group.
Article Synopsis
- - The study aimed to investigate how glycosides from Tripterygium wilfordii (GTW) affect genes related to testicular reproduction in male mice and the potential benefits of kidney-tonifying Chinese herbs.
- - Mice were treated with GTW to induce reproductive dysfunction, then various groups received different combinations of saline, GTW, and Chinese herbs (Cistanche and Rehmannia) for three weeks to assess changes in gene expression.
- - Results showed that GTW reduced the expression of certain reproductive genes and increased apoptosis-related genes; however, the Chinese herbs improved reproductive functions, especially when used together.