Lefty promotes the proliferation and invasion of trophoblast cells by inhibiting nodal expression.

Trophoblast cells play a vital role in embryo implantation. Exploring cytokines secreted by trophoblast cells will be significant in revealing the molecular mechanism of embryo implantation. In vitro-cultured trophoblast cells treated with Lefty revealed that Lefty could downregulate the expression of Nodal while promoting the expression of MMP-2 and MMP-9.

[Side effects of adjuvant chemotherapy and radiotherapy concurrently after conservative surgery for breast cancer].

Objective: Observe the side effects of adjuvant chemotherapy and radiotherapy concurrently after breast conservative surgery and investigate it feasible.

Methods: 40 breast cancer patients of conservative surgery were divided into two groups randomizedly, 20 patients of the study group were given adjuvant chemotherapy and radiotherapy concurrently, 20 patients of the control group were given adjuvant thermotherapy and radiotherapy sequently. Observe the side effects of the patients in the two groups and follow them 6 months.

[A prospective multicenter clinical trial of breast conserving therapy for early breast cancer in China].

Objective: To demonstrate the feasibility of breast conserving therapy (BCT) and establish a multimodality BCT model for early breast cancer in China.

Methods: A prospective multicenter case control study consisting of 4461 patients was carried out by the Cancer Hospital, Chinese Academy of Medical Sciences (CAMS) and nine other hospitals across China from November, 2001 to November, 2004, the result of BCT and mastectomy on early stage breast cancer were compared. Patients entry-primary tumor < or = 3 cm, primary tumor in periphery quadrant, pathology showed infiltrating carcinoma and clinical absence of locoregional lymphatic or distant metastasis.

[The effect of neoadjuvant chemotherapy on estrogen and progesterone receptor expression in breast carcinoma].

Objective: To investigate the effect of neoadjuvant chemotherapy on estrogen receptor (ER) and progesterone receptor (PR) expression in breast carcinoma.

Methods: Samples were obtained from 31 patients with breast carcinoma who received neo-adjuvant chemotherapy, ER or PR expressions were analyzed in preoperative core biopsies and final surgical specimens.

Results: ER level was up-regulated in 13 (41.

[Feasibility of breast conservation surgery after neoadjuvant chemotherapy for breast cancer].

Objective: To investigate the efficacy of neoadjuvant chemotherapy (NCT) and the feasibility of conservative breast surgery after reducing the size of a primary tumor by NCT in patients with operable breast cancer.

Methods: Thirty patients with stage IIB and IIIA breast cancer underwent NCT including epirubicin 60 mg/m(2) by intravenous injection on day 1 and paclitaxel 150 mg/m(2) by 3-hour continuous infusion on day 2 with 21 days as a cycle from July 2001 to April 2003. All patients received 3 - 4 cycles of NCT.

[The effect of transfecting Stat3beta cDNA on human breast cancer].

Objective: To study the effect of transfecting Stat3beta cDNA on human breast cancer.

Methods: Human breast cancer cells of the line SK-BR-3 were cultured and divided into 3 groups: Stat3beta transfection group (to be transfected with plasmid pIRES-Stat3beta containing Stat3beta by transient transfection technique), lipofectin reagent transfection group pIRES-EGFP transfection group, and control group. The positively transfected cells were isolated by fluorescence-activated cell sorter.

[Expression and significance of estrogen receptor isoforms in the development of human breast carcinoma].

Objective: To investigate the estrogen receptors (ER)alpha and ERbeta expression and their relationship with clinicopathological parameters in human breast carcinoma.

Methods: Samples were obtained from 30 breast carcinoma, reverse transcriptase polymerase chain reaction was used to measure the expression of ERalpha and ERbeta mRNA.

Results: ERalpha mRNA level was up-regulated in breast carcinoma tissue compared with adjacent normal tissue (t = 7.

[Significance of concentration of serum soluble vascular cell adhesion molecule-1 in epithelial ovarian carcinoma].

Background & Objective: It was reported that vascular cell adhesion molecule-1 (VCAM-1) was highly expressed in ovarian carcinoma samples. The latest research showed that the concentration of serum soluble VCAM-1 (sVCAM-1) was increased in the patients with various tumors. The aim of this study was to investigate the biological effects of sVCAM-1 on epithelial ovarian carcinoma.