Publications by authors named "Xin-long Zhu"
Objective: To investigate the biochemical characteristic of the neurons associated Zusanli (ST 36) in the rat by using Alexa Fluor 594 conjugated cholera toxin subunit B (AF594-CTB) neural tracing and calcitonin gene-related peptide (CGRP) fluorescent immunohistochemical techniques.
Methods: Four male Sprague Dawley rats were injected with AF594-CTB into the corresponding area of the Zusanli in the human body. After 3 surviving days, the rat's spinal cord and dorsal root ganglia (DRGs) at lumbar segments were dissected following perfusion with 4% paraformaldehyde, cut into sections, and then stained with CGRPfluorescent immunohistochemical method.
Objective: To observe the distribution of neurons and their axonal projection associated with "Yongquan" (KI 1) area by neural tracing technique with cholera toxin subunit B (CTB).
Methods: A total of 5 microL of 1% CTB solution was injected into the front central part of hind foot plantar (corresponding to the KI 1 region in the human body) using a Hamilton microsyringe. After 3 survival days, the rats were deeply anesthetized and transcardically perfused for collecting the dorsal root ganglia (DRGs), spinal cord and brain tissues.
Objectives: This study was performed to investigate the innervations related to acupuncture point PC8 in rats using a neural tracing technique.
Methods: After 6 μL of 1% cholera toxin subunit B (CTB) was injected into the site between the second and third metacarpal bone in rats, a corresponding site to acupuncture point PC8 in the human body, CTB labelling was examined with immunofluorescence and immunohistochemistry in the dorsal root ganglia (DRG), spinal cord and brainstem.
Results: All CTB labelling appeared on the ipsilateral side of the injection.
Background: The afferent projections from the auricular branch of the vagus nerve (ABVN) to the nucleus tractus solitaries (NTS) have been proposed as the anatomical basis for the increased parasympathetic tone seen in auriculo-vagal reflexes. As the afferent center of the vagus nerve, the NTS has been considered to play roles in the anticonvulsant effect of cervical vagus nerve stimulation (VNS). Here we proposed an "auriculo-vagal afferent pathway" (AVAP), by which transcutaneous auricular vagus nerve stimulation (ta-VNS) suppresses pentylenetetrazol (PTZ)-induced epileptic seizures by activating the NTS neurons in rats.
View Article and Find Full Text PDFObjective: To study the specific correlation between "Jinggu" (BL 64) and "Dazhong" (KI 4) in the nervous system by using a double-labeling of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594 (CTB-Alexa 488, 594) in rats, so as to investigate its neuroanatomical basis for clinical joint-application of Yuan-Source and Luo acupoints.
Methods: Three male SD rats were used in the present study. Under anesthesia (10% urethane), 0.
Objective: To investigate neuroanatomical characteristics of the primary sensory afferent and the motor neurons coming from and innervating acupoint "Chengshan" (BL 57) area in the rat by using cholera toxin subunit B conjugated with Alexa Fluor 488 (CTB-Alexa 488), a new generation of fluorescent neural tracing reagent.
Methods: Four male SD rats were used in the present study. Under anesthesia, 0.