Transformation mechanism of ions on different waters in alpine region.

The study investigates transformation mechanism of ions on different waters in Alpine region through analyzed the hydrochemical characteristics of the major ions of precipitation, glacier and snow meltwater, supra-permafrost water and river water in permafrost regions in the Tibetan Plateau under climate warming. The results showed that, The relation between recharge and discharge was the major ways for ionic transformation of each water body. Precipitation and glacier and snow meltwater are the main input sources for ionic transformation, and river water is the final output source.

The sources of supra-permafrost water and its hydrological effect based on stable isotopes in the third pole region.

The sources of supra-permafrost water and its hydrological effects were studied, based on the presence of stable isotopes in 562 samples collected in different ablation periods from the source regions of the Yangtze River. The δO (δD and d-excess) values for the initial ablation, ablation, and end ablation periods were -10.18‰ (-71.

[Diversity study of arbuscular mycorrhizal fungi of Coptis deltoidea].

Objective: To investigate the resources and distribution of Arbuscular Mycorrhizal Fungi (AMF) in rhizosphere of Coptis deltoidea.

Methods: The colonization situation of AMF of Coptis deltoidea were detected by acid fuchsin staining and then calculated the colonization rate. AMF fungal spores were obtained by Wet-screening method and then classified and identified by their morphological characteristics and histochemical staining method.

[A prospective cohort study on the influence of high doses of herbs for clearing heat and resolving stasis on survival rates in patients with hepatitis B-related acute-on-chronic liver failure].

Background: Hepatitis B-related acute-on-chronic liver failure (ACLF) is a severe clinical syndrome characterized by jaundice, coagulopathy, ascites and hepatic encephalopathy and with a high mortality rate of 65% to 93%. It involves significant ethical issues when a randomized, double-blinded, placebo-controlled clinical study is conducted to such a serious disease. Therefore, a prospective cohort study design was utilized to explore a new treatment modality of applying integrated traditional Chinese and Western medicine.

[The correlation between expression level of femB and resistance phenotype of methicillin-resistant Staphylococcus aureus (MRSA)].

Objective: To investigate the correlation between the phenotype and expression level of femB of Staphylococcus aureus (MRSA), to discuss the mechanism of different phenotypes in methicillin-resistant Staphylococcus aureus.

Methods: The minimal inhibitory concentrations (MICs) of oxacillin against 71 clinical isolates of Staphylococcus aureus were determined by agar dilution method according to NCCLS. The production of beta-lactamase was identified by Cefinase paper strip method.

[Preliminary study on genotyping of Mycobacterium tuberculosis strains isolated in Tibet with multiple locus variable numbers of tandem repeats].

Objective: To investigate the application of the multiple locus variable numbers of tandem repeats (MLVA) in genotype Mycobacterium tuberculosis strains isolated from Tibet, and to understand the characteristics of genotype and distribution.

Methods: 217 M. tuberculosis strains were collected from six regions of Tibet.

[Study on the correlation between resistance phenotype and expression level of femA of Staphylococcus aureus].

Objective: To detect the expression level of femA of Staphylococcus aureus strains with different phenotype.

Methods: 15 strains of the non-beta-lactamase-producing clinical isolates with different phenotype by agar dilution and by nitrocephin paper strip method were chosen as the object of test, in addition to 4 donative strains (BB270, BB308, BB586, COL). Total RNA were extracted and analysed by agarose gel electrophoresis.

[A study on the mechanism of drug resistance in Staphylococcus aureus].

Objective: To inquire into the mechanism of drug resistance in Staphylococcus aureus.

Methods: A total of 198 strains of Staphylococcus aureus were isolated from the samples sent to the Clinical Laboratory of Microbiology,West China Hospital. The resistance of Staphylococcus aureus to Methicillin was assayed with agar dilution.

[Effect of polylactic acid on the proliferation and differentiation of osteoblast-like cells].

Objective: To assess the effects of polylactic acid (PLA) on the proliferation and differentiation of UMR-106 osteoblast-like cells.

Methods: The ultrastructures of the surface of untreated and pretreated PLA films were observed under electron microscope. UMR-106 osteoblast-like cells were cultured with PLA film which was untreated or treated by some special agents together, then the cells morphology was observed, and methyl thiazolyl tetrazolium (MTT) assay and alkaline phosphatase (ALP) analysis were performed to detect the Tiny distinction between the untreated film and pretreated film was proliferation and differentiation.