Differential proteomic analysis reveals the mechanism of Musa paradisiaca responding to salt stress.

Salinity is one of the most important abiotic stresses, which affects the yield and quality of banana (Musa paradisiaca). To understand the salinity tolerance mechanisms of banana, the iTRAQ technique is employed to reveal the proteomic response of Brazil banana under different durations of 60 mmol/L NaCl stress. We have identified 77 DEPs and classified them into nine functional categories, compared with control (0 mmol/L NaCl treatment).

SRPK1 gene silencing promotes vascular smooth muscle cell proliferation and vascular remodeling via inhibition of the PI3K/Akt signaling pathway in a rat model of intracranial aneurysms.

Objective: Intracranial aneurysm (IA) is a life threatening cerebrovascular disease characterized by phenotypic modulation of vascular smooth muscle cells (VSMCs) and loss of vessel cells. In addition to environmental factors, genetic factors have been proposed to be a critical factor in the onset and progression of IA. The present study investigates the effects of serine-arginine protein kinase 1 (SRPK1) on VSMC proliferation and apoptosis both in vivo and in vitro, as well as its role in vascular remodeling in vivo through PI3 K/Akt signaling in IA.

[Effects of spraying paclobutrazol at different stages on physiological characteristics, yield and quality of peanut].

To explore the optimum stage of spraying with paclobutrazol (PBZ) for different peanut cultivars under high yield condition, we investigated the effects of spraying PBZ at different stages on chlorophyll content, root activity, protective enzymes, nitrogen and carbon metabolism enzymes of leaves, pod yield and kernel quality of peanut, with Huayu 20 and Huayu 25 as materials. The results showed that spraying PBZ at different stages increased root activity, chlorophyll content, SOD, POD, CAT, SS, SPS, PEPC activities and decreased the MDA content, NR, GS, GDH and GOGAT activities for pod setting stage of two cultivars. These results suggested that the effects would be more obvious at earlier spray time.

[Effects of exogenous Ca on growth and development, physiology and yield of peanut under salt stress].

Huayu 22, one of the peanut (Arachis hypogaea) cultivars, was used as material in this study. Peanuts, which grew under normal conditions and 100 mmol·L NaCl stress, were treated with 0, 6, 12 mmol·L Ca(NO) respectively to elucidate the effects of exogenous calcium on peanut salt tolerance. The effects of different Ca concentrations on the physiological indices and yield of peanut during the whole growth period under salt stress were investigated in potted plants, with the aim to provide theoretical basis for the growth and production of peanut in saline soil.

[Effects of single-seed sowing on canopy microenvironment, photosynthetic characteristics and pod yield of peanut (Arachis hypogaca)].

The large-seed peanut cultivar of Huayu 22 was used to study the differences of canopy microenvironment, photosynthetic characteristics, and pod yield at three single-seed sowing densities, i.e., 225000 (S₁), 195000 (S₂) and 165000 (S₃) holes per hectare, in field experiments.

Peanut violaxanthin de-epoxidase alleviates the sensitivity of PSII photoinhibition to heat and high irradiance stress in transgenic tobacco.

This is the first study on peanut VDE, which led to multiple biochemical and physiological changes to heat and HI stress by improving de-epoxidation of the xanthophylls cycle. A peanut (Arachis hypogaea L.) violaxanthin de-epoxidase gene (AhVDE) was isolated by RT-PCR and RACE methods.

Isolation and functional characterisation of CDPKs gene from Arachis hypogaea under salt stress.

One of salt-induced calcium-dependent protein kinases (CDPKs) gene was isolated from Arachis hypogeae L. by RACE method. The cDNA full length was 2241bp deposited in GenBank (number KF437909), designated as AhCDPK.

Calcium contributes to photoprotection and repair of photosystem II in peanut leaves during heat and high irradiance.

In this study, we investigated the effects of exogenous calcium nitrate on photoinhibition and thylakoid protein level in peanut plants under heat (40°C) and high irradiance (HI) (1,200 µmol/m(2) per s) stress. Compared with control seedlings (cultivated in 0 mmol/L Ca(NO3 )2 medium), the maximal photochemical efficiency of photosystem II (PSII) in Ca(2+) -treated plants showed a slight decrease after 5 h stress, accompanied by lower degree of PSII closure (1-qP), higher non-photochemical quenching, and lower level of membrane damage. Ca(2+) inhibitors were used to analyze the varieties of antioxidant enzymes activity and PSII proteins.

Exogenous calcium alleviates photoinhibition of PSII by improving the xanthophyll cycle in peanut (Arachis hypogaea) leaves during heat stress under high irradiance.

Peanut is one of the calciphilous plants. Calcium (Ca) serves as a ubiquitous central hub in a large number of signaling pathways. The effect of exogenous calcium nitrate [Ca(NO3)2] (6 mM) on the dissipation of excess excitation energy in the photosystem II (PSII) antenna, especially on the level of D1 protein and the xanthophyll cycle in peanut plants under heat (40°C) and high irradiance (HI) (1 200 µmol m(-2) s(-1)) stress were investigated.

Microsurgical management of cerebral parenchymal cysticercosis.

Objective: Microsurgery is an optional way to treat parenchymal neurocysticercosis. The aim of this study is to evaluate the therapeutic efficacy of microsurgery in cerebral parenchymal cysticercosis.

Materials And Methods: A retrospective analysis was performed of the clinical data and outcomes of microsurgery in 20 cases of cerebral parenchymal cysticercosis.

17Beta-estradiol differentially protects cortical pericontusional zone from programmed cell death after traumatic cerebral contusion at distinct stages via non-genomic and genomic pathways.

Pericontusional zone (PCZ) of traumatic cerebral contusion is a target of pharmacological intervention. Our previous study indicated that 17beta-estradiol has a protective role in PCZ after traumatic cerebral contusion via the upregulation of estrogen receptor (ER) alpha mRNA induction and protein expression as well as inhibition of caspase-3 activation, suggesting that genomic signaling pathway is implicated in the protective effect of 17beta-estrodiol. Recent findings demonstrated that 17beta-estradiol also acts on the extranuclear/membrane ER to activate non-genomic signaling pathway to regulate cellular functions and exert the protective effect in the brain.

[Expression of EVEC in ovarian carcinoma and its biological significance].

Objective: To investigate the expression of EVEC in ovarian carcinoma and explore its biological significance.

Methods: The expression of EVEC in 22 specimens of normal ovarian tissues and 63 specimens of ovarian cancers was detected by RT-PCR and Western blotting analysis, respectively.

Results: RT-PCR showed that the expression level of EVEC in stage I-II ovarian cancer (0.

Development of the nine X-STR loci typing system and genetic analysis in three nationality populations from China.

This study is to develop a new multiplex polymerase chain reaction (PCR) system that simultaneously amplifies the nine X-chromosome short tandem repeats loci in the same PCR reaction, and to explore their polymorphism and mutation rate among three nationality populations from China. These loci included DXS6854, DXS9902, DXS6809, GATA172D05, HPRTB, DXS7423, DXS6807, DXS8378, and DXS8377. The samples of 890 (484 males and 406 females) unrelated individuals from Guangdong Han population, Xinjiang Uigur, and Inner-Mongolia Mongol were successfully analyzed using this multiplex system.

Overexpression of Arabidopsis CBF1 gene in transgenic tobacco alleviates photoinhibition of PSII and PSI during chilling stress under low irradiance.

A known arabidopsis cDNA clone, the CRT/DRE binding factor 1 (CBF1), was isolated and introduced into tobacco plants. It has been reported that CBF1 is one member of CBF gene family related to low temperature and enhancing low temperature tolerance of plants. In the present work, the transcripts could be detected in the transgenic lines.

[Effects of different calcium salts on freezing resistance of satsuma mandarin].

The study on the effects of foliar spraying 20 mmol x L(-1) of CaCl2, Ca (NO3)2 and CaAc2 on the freezing resistance of satsuma mandarin (Citrus unshiu Marc. cv. Guoqing No.

[Mechanism and effect of selective cyclooxygenase-2 inhibitor NS-398 on human cervical carcinoma cells].

Objective: To investigate the effect and mechanism of the selective COX-2 inhibitor NS-398 and carboplatin on the human cervical carcinoma cell line Hela.

Methods: The effect of NS-398, carboplatin, and both on the proliferation of Hela cells was assessed by methyl-thiazolyl tetrazolium (MTT) method, and the apoptosis assay and cell cycle distribution were analyzed by flow cytometry.

Results: NS-398, and carboplatin inhibited the growth of Hela cells in a dose and time-dependent manner.

[Surgical pattern of radical hysterectomy and pelvic lymph node dissection for patients with cervical cancer].

Objective: To investigate the probability of improving radical surgery to resect uterus thoroughly and to decrease various complications after the surgery.

Methods: We compared the clinical effect of reformed radical hysterectomy for 79 patients with the effect of tradition radical hysterectomy for 60 patients. Reformed surgery had the following features: We firstly resected the uterus and then dissected the pelvic lymph node.

[Study on the theory and experiments of radiative parameters of the Eu(3+) ion in transparent optical resin].

The optical properties of a transparent optical resin (HMA/ST) containing ternary rare earth complex are reported. Based on Judd-Ofelt (J-O) theory, the J-O parameters were calculated to be omega2 = 10.139 4 x 10(-20) cm2, omega4 = 3.

Overexpression of CaHSP26 in transgenic tobacco alleviates photoinhibition of PSII and PSI during chilling stress under low irradiance.

A sweet pepper cDNA clone, CaHSP26 encoding the chloroplast (CP)-localized small heat shock protein (sHSP), was isolated and characterized with regard to its sequence, response to various temperatures and function in transgenic tobacco plants. The deduced amino acid sequence contained three highly conserved regions, showing high identities with other plant sHSPs. Expression of the CaHSP26 gene showed that the mRNA accumulation of CaHSP26 was induced by heat stress.

[Expression of transforming growth factor-beta1 in cervical squamous carcinoma cell and stroma].

Objective: To explore the significance and expression of transforming growth factor-beta1 (TGFbeta1) in cervical squamous carcinoma.

Methods: The expression of TGFbeta1 in 31 cases of cervical squamous carcinomas were detected by SP immunohistochemical staining.

Results: TGFbeta1 expression was found in both cytoplasm and extracellular stroma.

[Differential expression on cell wall formative genes in cell suspension cultures of Populus tomentosa Carr].

With fine and granular suspension cultures established from P. tomentosa, differential expression of 12 genes related to cell wall formation including cell expansion, cellulose synthesis, lignin synthesis, etc, were investigated by reverse transcript PCR (RT-PCR) technique. Fine suspension cells showed high expression on 3 alpha-Exp genes with the highest alpha-Exp2 followed by alpha-Exp3 and alpha-Exp1 suggesting their strong cell expansion, while granular suspension cells only exist high expression on alpha-Exp3 indicating their later period of cell expansion or initial stage of secondary cell wall formation.