Publications by authors named "Xin-feng Huang"
Stable SET knockdown in breast cell carcinoma inhibits cell migration and invasion.
Biochem Biophys Res Commun
October 2014
Breast cancer is the most malignant tumor for women, however, the mechanisms underlying this devastating disease remain unclear. SET is an endogenous inhibitor of protein phosphatase 2A (PP2A) and involved in many physiological and pathological processes. SET could promote the occurrence of tumor through inhibiting PP2A.
View Article and Find Full Text PDF[Study on sperm damage caused by trichloroethylene in male rats].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
November 2013
Article Synopsis
- The study aimed to investigate how trichloroethylene exposure affects sperm health in male rats.
- Rats were exposed to different concentrations of trichloroethylene, and their sperm was analyzed for motility, morphology, and mitochondrial health.
- Results showed that higher concentrations of trichloroethylene significantly decreased sperm motility and increased sperm abnormalities and apoptosis rates.
[Effects of trichloroethylene on hepatotoxicity in cytochrome 2E1-silenced hepatocytes].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
March 2013
Objective: To prepare cytochrome (CYP)2E1-silenced hepatocytes by lentivirus-mediated RNA interference technology and to investigate the hepatotoxicity of trichloroethylene (TCE) in CYP2E1-silenced hepatocytes.
Methods: Short hairpin RNA fragments were designed and synthesized and were then ligated into the lentiviral vector; single colonies were screened; the plasmid was extracted after PCR and sequence identification and then transferred into L02 hepatocytes; the CYP2E1-silenced hepatocytes were selected; real-time quantitative PCR and Western blot were used to evaluate the interference effects. The obtained CYP2E1-silenced hepatocytes, as well as normal L02 hepatocytes, were treated with TCE (0, 0.