Nitrogen reduction reaction (NRR) as a promising approach to ammonia synthesis has received much attention in recent years. Molybdenum disulfides (MoS), as one of the most potential candidates for NRR, are extensively investigated. However, the inert basal plane limits the application of MoS.
View Article and Find Full Text PDFBackground: Total hip arthroplasty (THA) induces postoperative pain in elderly individuals. The erector spinae plane block (ESPB) is a novel analgesic approach for postoperative pain control. This randomized controlled trial evaluated the effectiveness of ultrasound-guided ESPB with ropivacaine in reducing pain in elderly patients undergoing THA.
View Article and Find Full Text PDFPlasmon-mediated chemical reactions (PMCR) have garnered growing interest as a promising concept for photocatalysis. However, in electrochemical systems at solid-liquid interfaces, the photo-induced charge transfer on the surface of metal-semiconductor heterostructures involves complex processes and mechanisms, which are still poorly understood. We explore the plasmon-mediated carrier transfer mechanism and the synergistic effect of light and electric fields on Ag-TiO heterostructures, through a combination of electrochemical surface-enhanced Raman spectroscopy and photoelectrochemical methods, with para-aminothiophenol (PATP) serving as a probe molecule.
View Article and Find Full Text PDFSpinal cerebrospinal fluid (CSF) volume is the primary determinant for the spread of spinal anesthesia. However, it cannot generally be obtained during spinal anesthesia, and patient physical characteristics are always adopted to obtain a suitable spinal spread. In this study, we sought to explore the relationship between individual physical characteristics and thoracosacral CSF volume to provide a theoretical basis for more accurate spinal anesthesia.
View Article and Find Full Text PDFPerioperative neurocognitive disorder (PND) is a common complication associated with anesthesia and surgery in the elderly. The dysfunction of transient receptor potential vanilloid 4 (TRPV4) has been associated with a number of diseases, including Alzheimer's disease. Given that ketamine can reportedly improve PNDs, the present study sought to determine whether ketamine-induced PND alleviation was mediated by activation of TRPV4 channel opening.
View Article and Find Full Text PDFsubsp. originating in reptiles can cause invasive infections in humans. Here, we present the whole-genome sequence of subsp.
View Article and Find Full Text PDFYing Yong Sheng Tai Xue Bao
February 2017
With increasing human disturbances (e.g., spatial fragmentation), drastic changes have taken place in spatial structure, which further affects the structure and function of ecosystem.
View Article and Find Full Text PDFZhonghua Yu Fang Yi Xue Za Zhi
January 2013
Objective: To investigate the molecular epidemiological characteristics of norovirus in Guangzhou from 2009 to 2011.
Methods: A total of 183 water samples, 1162 seafood samples and 1066 diarrhea stool specimens were collected from January 2010 to May 2011, June 2009 to June 2011 and July 2009 to December 2010 respectively in Guangzhou. Norovirus was detected by real time reverse transcript-PCR (qRT-PCR).
Objective: To document the investigation and control of an outbreak of gastroenteritis in City G, South China, and provide a reference for preventing future outbreaks.
Methods: An ambispective cohort study was designed. Attack rate (AR) and relative risks (RR) were calculated to identify the causes of gastroenteritis.
Bing Du Xue Bao
September 2010
To investigate the inhibitory effect of RNA interference (RNAi) on dengue virus I (DENV-1) replication. Small interfering RNA (siRNA) against the PreM gene of dengue virus was synthesized and transfected into C6/36 cells with liposome, which was then attacked by DENV-1 virus. The antiviral effect of siRNA was evaluated by cytopathic effect (CPE), the cell survival rate measured by MTT, and virus RNA quantified by real-time RT-PCR.
View Article and Find Full Text PDFObjective: To study the first locally identifed A/H1N1 secondary cases outbreak in China.
Methods: Interview and field investigation were integrated to describe the whole process of transmission on each case and to illustrate the relationships between the onset of the disease and the retated factors.
Results: Two contact persons appearanced fever and whose throat swabs were tested positive to H1N1 viral nucleic acid.
Nan Fang Yi Ke Da Xue Xue Bao
November 2009
Objective: To study the relation of the detection rates of the novel influenza virus A/H1N1 RNA in clinically confirmed patients in the 2009 pandemic with the age distribution of the patients and the disease course.
Methods: A total of 151 clinical patients with H1N1 infection were enrolled in this study, from whom 833 dynamic throat swab samples were obtained for detecting the H1N1 RNA using real-time PCR. A statistical analysis of the age distribution was performed among the patients with different disease courses.
To understand the genetic characteristics of hemagglutinin (HA) and neuraminidase (NA) of type B influenza viruses in Guangzhou in 2006, three virus strains from etiology surveillance and seven strains from outbreaks were investigated. Genome RNAs of type B influenza viruses were extracted and reverse-transcripted into cDNAs using random primers. The whole-length DNA of HA and NA were amplified by polymerase chain reaction (PCR), cloned into T-A plasmid and sequenced, and analyzed phylogenetically by DNAstar software.
View Article and Find Full Text PDFZhonghua Liu Xing Bing Xue Za Zhi
November 2006
A patient may have been infected with highly pathogenic avian influenza virus H5N1 in Guangzhou, People's Republic of China, at a food market that had live birds. Virus genes were detected in 1 of 79 wire cages for birds at 9 markets. One of 110 persons in the poultry business at markets had neutralizing antibody against H5N1.
View Article and Find Full Text PDFZhonghua Liu Xing Bing Xue Za Zhi
January 2005
Objective: To compare the 4 test kits on severe acute respiratory syndrome coronavirus (SARS-CoV) gene, antigen and antibody for early diagnose of SARS patients.
Methods: Three enzyme linked immunosorbent assay (ELISA) kits were used to detect SARS-CoV IgG, IgM and N protein and fluorescent polymerase chain reaction (F-PCR) kit was used to detect SARS-CoV RNA.
Results: In 162 serum samples, 90.
Proc Natl Acad Sci U S A
February 2005
The genomic sequences of severe acute respiratory syndrome coronaviruses from human and palm civet of the 2003/2004 outbreak in the city of Guangzhou, China, were nearly identical. Phylogenetic analysis suggested an independent viral invasion from animal to human in this new episode. Combining all existing data but excluding singletons, we identified 202 single-nucleotide variations.
View Article and Find Full Text PDFAccurate and timely diagnosis of severe acute respiratory syndrome coronavirus (SARS-CoV) infection is a critical step in preventing another global outbreak. In this study, 829 serum specimens were collected from 643 patients initially reported to be infected with SARS-CoV. The sera were tested for the N protein of SARS-CoV by using an antigen capture enzyme-linked immunosorbent assay (ELISA) based on monoclonal antibodies against the N protein of SARS-CoV and compared to 197 control serum samples from healthy donors and non-SARS febrile patients.
View Article and Find Full Text PDFSichuan Da Xue Xue Bao Yi Xue Ban
January 2004
Objective: To compare the effects of different HDL subclasses on the efflux of 3H-cholesterol loaded human smooth muscle cells (SMCS).
Methods: 3H-cholesterol loaded human SMCS were incubated with purified pre-beta 1 HDL, alpha-HDL and apoE-deficient HDL3 respectively. The cholesterol effluxing capacity and incubating time of different HDL subclasses were detected, and were analysed by double-reciprocal mapping.
Zhonghua Liu Xing Bing Xue Za Zhi
May 2003
Objective: To analyze the epidemiological characteristics, related risk factors, measures for its control of severe acute respiratory syndrome (SARS).
Methods: Data on epidemiological features, pathogens and measures for control were collected and analyzed.
Results: Since Jan 2003, infectious atypical pneumonia (AP) has become epidemic in Guangzhou city.