Mutation spectrum of germline cancer susceptibility genes among unselected Chinese colorectal cancer patients.

Genetic factors play an important role in colorectal cancer (CRC) risk, yet the prevalence and spectrum of germline cancer susceptibility gene mutations among unselected Chinese CRC patients is largely undetermined. We performed next-generation sequencing with a 73-genes panel and analyzed the prevalence and spectrum of germline mutations in 618 unselected Chinese CRC patients. We classified all identified germline alterations for pathogenicity and calculated the frequencies of pathogenic mutations.

[Degradation kinetics of naphthalene by anaerobic sludge and analysis of the bacterial biodiversity].

For efficient biological treatment of naphthalene in the industrial wastewater, activated anaerobic sludge was collected from a wastewater treatment plant of petroleum industry, and domesticated with naphthalene, naphthalene and lactate as electron donors, respectively. When the removal efficiency of naphthalene reached more than 90% in a domestication cycle, degradation kinetics were investigated in batch reactions with naphthalene, naphthalene and lactate as electron donors, respectively. Meanwhile, the microbial DNA was extracted from the sludge with high naphthalene removal efficiency, the 16S rDNA clone library was built up, and the bacterial community was analyzed.