Enhancing Cordyceps Sinensis shelf life: The role of liquid nitrogen spray freezing in maintaining hypha structure and reducing metabolic degradation.

Cordyceps sinensis (C. sinensis) is a valuable edible fungus, known for its therapeutic benefits, including immune enhancement and anti-inflammatory effects, making it an important component in nutritional applications. However, its delicate nature makes long-term storage challenging, with conventional freezing often leading to the loss of bioactive compounds.

The Association Between Insomnia and Temporomandibular Disorders in Orthodontic Patients.

Objective: This cross-sectional study aimed to investigate the association between insomnia and the presence of temporomandibular disorders (TMD) and its subtypes in orthodontic patients.

Methods: A total of 648 adult orthodontic patients (158 males and 490 females, median age 26) were included and completed a questionnaire containing sociodemographic information, insomnia severity index (ISI), the five major temporomandibular disorder symptoms (5Ts) checklist, and self-reported sleep bruxism. Presence of insomnia and TMD of the included patients was determined according to the diagnostic criteria, and statistical analyses were conducted as appropriate to compare ISI-related scores between TMD and non-TMD participants.

The Association of Occipital Spur with Craniocervical Posture and Craniofacial Morphology.

Purpose: This cross-sectional study aimed to evaluate the relationship between occipital spur (OS) with both craniocervical posture and craniofacial morphology.

Methods: The study involved 240 lateral cephalograms from subjects with and without OS. The craniocervical posture and facial morphology of every individual were assessed through Uceph software analysis of their cephalograms, considering 32 variables.

Associations between oral behaviors, temporomandibular disorder subtypes and psychological distress in adult women: a retrospective case-control study.

Oral behaviors and psychological distress are known to be related to temporomandibular disorders (TMDs). However, the relationship between various oral behaviors and specific TMD subgroups in adult women experiencing psychological distress is still unclear. To investigate the relationship between various oral behaviors and different TMD subgroups with different psychological distress states.

Simplified Jaw Functional Limitation Scale for Temporomandibular Disorders: Psychometric Evaluation.

Objective: This study aimed to simplify the Jaw Functional Limitation Scale (JFLS) and evaluate its reliability and validity in assessing jaw function in patients with temporomandibular disorders (TMD) in primary care.

Methods: TMD patients and non-TMD participants were assessed using Diagnostic Criteria for Temporomandibular Disorders (DC/TMD). Demographic data and the 8-item JFLS questionnaire were collected to quantify jaw functional restriction.

Application of Solution-Processed High-Entropy Metal Oxide Dielectric Layers with High Dielectric Constant and Wide Bandgap in Thin-Film Transistors.

High-k metal oxides are gradually replacing the traditional SiO dielectric layer in the new generation of electronic devices. In this paper, we report the production of five-element high entropy metal oxides (HEMOs) dielectric films by solution method and analyzed the role of each metal oxide in the system by characterizing the film properties. On this basis, we found optimal combination of (AlGaTiYZr)O with the best dielectric properties, exhibiting a low leakage current of 1.

Prognostic implications and therapeutic opportunities related to CAF subtypes in CMS4 colorectal cancer: insights from single-cell and bulk transcriptomics.

Cancer-associated fibroblasts (CAFs) significantly influence tumor progression and therapeutic resistance in colorectal cancer (CRC). However, the distributions and functions of CAF subpopulations vary across the four consensus molecular subtypes (CMSs) of CRC. This study performed single-cell RNA and bulk RNA sequencing and revealed that myofibroblast-like CAFs (myCAFs), tumor-like CAFs (tCAFs), inflammatory CAFs (iCAFs), CXCL14CAFs, and MTCAFs are notably enriched in CMS4 compared with other CMSs of CRC.

Off-pump coronary artery bypass grafting versus percutaneous coronary intervention with second-generation drug-eluting stents for coronary artery revascularization.

Objective: This study compared the clinical efficacy of off-pump coronary artery bypass grafting (OPCAB) with percutaneous coronary intervention (PCI) in the treatment of left main coronary artery and/or triple-vessel disease (LM and/or TVD).

Methods: We retrospectively enrolled 1484 consecutive patients with LM and/or TVD in Shanxi Cardiovascular Hospital from January 2015 to December 2022 and divided them into the OPCAB group (n = 583) and the PCI group (with second-generation drug-eluting stents) (n = 901). Propensity score matching was used for 316 equally matched pairs of patients in the groups.

Development, validation, and clinical application of LC-MS/MS method for simultaneous determination of ibrutinib, zanubrutinib, orelabrutinib, acalabrutinib, and their active metabolites in patients with B-cell lymphoma.

Bruton's tyrosine kinase inhibitors (BTKis) exhibit significant interindividual pharmacokinetics, making therapeutic drug monitoring (TDM) a promising approach for personalized therapy. However, simultaneous quantification of multiple BTKis poses technical challenges. A unified protocol for BTKis detection would be clinically desirable.

Condylar osseous changes following conservative therapies: A cone-beam computed tomography longitudinal study on adult patients with degenerative temporomandibular joint disease.

This retrospective study aimed to comprehensively investigate the impact of non-surgical treatments on condylar osseous changes in adult patients with degenerative joint disease (DJD). Radiographic and clinical data were collected for analysis. Cone-beam computed tomography (CBCT) was used to diagnose DJD, including flattening, erosion, osteophytes, sclerosis and cysts.

Onboard measurements of organic vapor emissions from river vessels under various operational conditions.

The emission factors and characteristics of pollutants from river vessels are critical for understanding the environmental impact of ship emissions, particularly in inland waterways. However, research gaps remain regarding emissions of volatile organic compounds (VOCs) and intermediate-volatility organic compounds (IVOCs) from river vessels. In this study, we collected and analyzed organic vapor emissions, including non-methane hydrocarbon (NMHCs), oxygenated volatile organic compounds (OVOCs) and IVOCs, from three river vessels under different operating conditions.

Sex differences in craniofacial parameters of children and adolescents: a comparative study with the maturation of cervical vertebrae using a cephalometric method.

This study aimed to investigate sex differences in craniomaxillofacial parameters in children and adolescents. Lateral cephalograms were obtained from 340 subjects (141 male, 199 female) aged 6 to 18 years, and their craniofacial characteristics and cervical vertebral maturity were assessed using the quantitative cervical vertebral maturity (QCVM) method. Age-based stratified analyses on craniofacial parameters and further comparison in QCVM Ⅲ subjects were performed.

Research on prediction of PPV in open pit mine used on intelligent hybrid model of extreme gradient boosting.

Peak particle velocity (PPV) serves as a critical metric in assessing the appropriateness of blasting design parameters. However, existing methods for accurately measuring PPV remain insufficient. To develop a robust PPV prediction model, this study integrates the Extreme Gradient Boosting (XGBoost) algorithm with four distinct optimization techniques: Runge Kutta Optimizer (RUN), Equilibrium Optimizer (EO), Gradient-Based Optimizer (GBO), and Reptile Search Algorithm (RSA).

Multisource representation learning for pediatric knowledge extraction from electronic health records.

Electronic Health Record (EHR) systems are particularly valuable in pediatrics due to high barriers in clinical studies, but pediatric EHR data often suffer from low content density. Existing EHR code embeddings tailored for the general patient population fail to address the unique needs of pediatric patients. To bridge this gap, we introduce a transfer learning approach, MUltisource Graph Synthesis (MUGS), aimed at accurate knowledge extraction and relation detection in pediatric contexts.

The Association Between Specific Oral Behaviors and the Number of Temporomandibular Disorder Symptoms in the General Population: A Cross-Sectional Study.

Objective: This study aimed to thoroughly explore the relationship between individual oral behavior and the differing numbers of temporomandibular disorder (TMD) symptom in the general population.

Methods: A total of 565 participants were recruited and completed a questionnaire containing demographic characteristics, eight specific oral behaviors, and five major TMD symptoms (5Ts) checklist. Multivariate linear regression analysis was performed to assess the relationship between individual oral behavior and the number of TMD symptoms, after controlling for confounders.

The Association Between Temporomandibular-Related Quality of Life and Oral Behaviours: A Cross-Sectional Study in Patients With Temporomandibular Disorders.

Objectives: Oral behaviours (OB) are some oral overuse behaviours which could be observed in patients with temporomandibular disorders (TMD). This study aims to investigate the association between TMD-related quality of life and OB to enhance understanding of these behaviours.

Methods: A total of 319 participants diagnosed with TMD were included in this research.

MAP3K1 mutations confer tumor immune heterogeneity in hormone receptor-positive HER2-negative breast cancer.

Treatment for hormone receptor-positive/human epidermal growth factor receptor 2-negative (HR+/HER2-) breast cancer, the most common type of breast cancer, has faced challenges such as endocrine therapy resistance and distant relapse. Immunotherapy has shown progress in treating triple-negative breast cancer, but immunological research on HR+/HER2- breast cancer is still in its early stages. Here, we performed a multi-omics analysis of a large cohort of patients with HR+/HER2- breast cancer (n = 351) and revealed that HR+/HER2- breast cancer possessed a highly heterogeneous tumor immune microenvironment.

DeSide: A unified deep learning approach for cellular deconvolution of tumor microenvironment.

Cellular deconvolution via bulk RNA sequencing (RNA-seq) presents a cost-effective and efficient alternative to experimental methods such as flow cytometry and single-cell RNA-seq (scRNA-seq) for analyzing the complex cellular composition of tumor microenvironments. Despite challenges due to heterogeneity within and among tumors, our innovative deep learning-based approach, DeSide, shows exceptional accuracy in estimating the proportions of 16 distinct cell types and subtypes within solid tumors. DeSide integrates biological pathways and assesses noncancerous cell types first, effectively sidestepping the issue of highly variable gene expression profiles (GEPs) associated with cancer cells.

Quantitative analysis of 4-hydroxy-2-nonenal (HNE) in fish muscle by high-performance liquid chromatography with pre-column derivatization using (2,4-dinitrophenyl)-hydrazine.

4-Hydroxy-2-nonenal (HNE), a reactive compound produced during the peroxidation of polyunsaturated fatty acids, is implicated in numerous diseases and the degradation of food quality. Although the detection of HNE in meat has a long history, methods for detecting HNE in freshwater fish are inadequate due to the significant influence of matrix differences (i.e.

GMNI: Achieve good data augmentation in unsupervised graph contrastive learning.

Graph contrastive learning (GCL) shows excellent potential in unsupervised graph representation learning. Data augmentation (DA), responsible for generating diverse views, plays a vital role in GCL, and its optimal choice heavily depends on the downstream task. However, it is impossible to measure task-relevant information under an unsupervised setting.

Vitamin D Levels and Temporomandibular Disorders: A Bidirectional Two-Sample Mendelian Randomization Analysis.

Objective: Growing researches explore vitamin D's role in temporomandibular disorders (TMDs), but the link between vitamin D and TMDs remains debated. To clarify the causal relationship, we conducted a Mendelian randomization (MR) analysis using data from genome-wide association studies (GWAS).

Subjects And Methods: The GWAS dataset of vitamin D (GWAS ID: ukb-d-30890_irnt; sample size: 329247) was obtained from the IEU Open GWAS project.

Evaluation of Dexmedetomidine with Tiletamine-Zolazepam for Snow Leopard (Panthera uncia) Immobilization.

This study describes two different doses of dexmedetomidine combined with tiletamine-zolazepam (TZ) for anesthesia in snow leopards (Panthera uncia). A total of 11 adult snow leopards were anesthetized in Xining City, Qinghai Province, China from November 2019 to June 2023. We recorded the onset time, anesthesia duration, head-up time, and walking time.

Development and validation of knowledge, attitude, and practice scale regarding antibiotic use for Chinese medical students.

Aims And Objectives: This study aimed to develop and validate a knowledge, attitude, and practice scale of antibiotic use for Chinese medical students.

Method: A five-phased procedure was adopted: (a) A item pool was constructed after literature review; (b) Delphi method was used to refine the items; (c) A pilot study was conducted to clarify the item; (d) Item analysis and exploratory factor analysis were used to finalize the scale; (e) Content validity, construct validity and reliability of the scale were analysed.

Results: The scale of 32 items, respectively 15, 9, and 8 for knowledge, attitude, and practice, was finalized.