Influence and progress of tea pigment research: A comprehensive analysis of application of bibliometrics.

Tea pigment, as a natural pigment component in tea, has attracted much attention because of its unique health benefits. In recent years, with the deepening of scientific research, the research on biological activity, extraction technology and application of tea pigment has made remarkable progress. Through systematic bibliometrics analysis, this paper comprehensively combs and evaluates the research status of tea pigment.

Low Rh doping accelerated HER/OER bifunctional catalytic activities of nanoflower-like Ni-Co sulfide for greatly boosting overall water splitting.

Facile synthesis of high-efficiency and stable bifunctional electrocatalyst is essential for producing clean hydrogen in energy storage systems. Herein, low Rh-doped flower-like NiS/CoS heterostructures were facilely prepared on porous nickel foam (labeled Rh-NiS/CoS/NF) by a hydrothermal method. The correlation of the precursors types with the morphological structures and catalytic properties were rigorously investigated for oxygen evolution reaction (OER) and hydrogen evolution reaction (HER) in the control groups.

A systematic review of tea pigments: Prevention of major diseases, protection of organs, and potential mechanisms and applications.

With the growing awareness of a healthy life, tea pigments (TPGs) are in focus for their health benefits. TPGs not only provide specific color to tea liquor but also possess health benefits such as anti-obesity, anti-tumor, anti-inflammatory, anti-viral, anti-oxidative, and bacteriostatic properties. Also, TPGs can benefit bone, liver, kidney, cardiovascular, gut microbiome, and sleep health.

Excision and integration of unconventional circularizable structures involving the erm(B) gene in enterococci.

Traditionally, insertion sequences (ISs) play a major role in disseminating antimicrobial resistance genes (ARGs) in bacteria through transposition and translocation, forming regions that contain multiple ARGs flanked by single or multiple copies of IS. In addition, unconventional circularizable structures (UCSs), lacking recombinase genes but being surrounded by directly repeated sequences (DRs) of various sizes which do not contain transposase genes, were reported to be involved in the dissemination of ARGs. In this study, a novel UCS was identified on plasmid pE508-2 in E.

Cross-species transmission of an emerging porcine circovirus (PCV4): First molecular detection and retrospective investigation in dairy cows.

Porcine circovirus 4 (PCV4), a novel porcine circovirus identified in pigs, has recently been proved to be pathogenic to piglets. However, little is known about its cross-species transmission, and demonstration of PCV4 in dairy cows is lacking. To explore whether the PCV4 genome exists in dairy cows, 1170 fecal samples were collected from dairy farms in 7 cities in Henan Province of China during 2012-2021, and screened by qPCR for the presence of PCVs (PCV2-PCV4).

Theophylline-regulated pyrolysis synthesis of nitrogen-doped carbon nanotubes with iron-cobalt nanoparticles for greatly boosting oxygen reduction reaction.

At present, construction of economical, efficient and stable electrocatalysts for oxygen reduction reaction (ORR) is critical to alleviate the energy shortage. Theophylline (THP) can be easily extracted from natural plants, whose nitrogen atoms can chelate with metal ions. With assistance of THP, FeCo alloy was confined in N-doped carbon nanotubes (FeCo/NCNTs-800) by one-step pyrolysis of a mixture of the metal precursors, g-CN and THP.

Well entrapped platinum-iron nanoparticles on three-dimensional nitrogen-doped ordered mesoporous carbon as highly efficient and durable catalyst for oxygen reduction and zinc-air battery.

The high-performance and durable oxygen reduction reaction (ORR) catalyst on air cathode is a key component in assembly of Zn-air batteries. Herein, three-dimensional N-doped ordered mesoporous carbon (3D N-OMC) was first prepared with silica as a template via pyrolysis with assistance of dicyandiamide as a N-doping agent, combined by full adsorption of platinum (II) acetylacetonate (Pt(acac)) and iron (II) phthalocyanine (FePc) via π-π interactions. After further pyrolysis of the resulting mixture, many PtFe nanoparticles were efficiently incorporated in 3D N-OMC (termed as PtFe@3D N-OMC for simplicity).

Water-regulated and bioinspired one-step pyrolysis of iron-cobalt nanoparticles-capped carbon nanotubes/porous honeycombed nitrogen-doped carbon composite for highly efficient oxygen reduction.

To achieve commercial applications of green fuel cells and rechargeable metal-air batteries, rational design and synthesis of low-cost, high-efficiency and ultra-stable transition metal-based electrocatalysts are of significant importance for alkaline oxygen reduction reaction (ORR). In this work, iron-cobalt (FeCo) nanoparticles-capped carbon nano-tubes/-porous nitrogen-doped honeycombed carbon composite (FeCo-CNTs/NHC-800) is synthesized by a water-regulated and bioinspired one-step pyrolysis method at 800 °C, where l-histidine behaves as the C and N sources combined by working as a chelating agent of Fe/Co. The formation mechanism is discussed by adjusting the pyrolysis temperature and water amount.

Isolation and Phylogenetic Analysis of Reemerging Pseudorabies Virus Within Pig Populations in Central China During 2012 to 2019.

To understand the biological characteristics of the reemerging pseudorabies virus (PRV) strains, a total of 392 tissue samples were collected from diseased pigs during reemerging PR outbreaks between 2012 and 2019 on farms in central China where swine had been immunized with Bartha-K61 and 51 (13. 01%) were positive for the gE gene by PCR. Sixteen PRV strains were isolated and caused clinical symptoms and death in mice.

Chondroitin Sulfate Alleviates Diabetic Osteoporosis and Repairs Bone Microstructure Anti-Oxidation, Anti-Inflammation, and Regulating Bone Metabolism.

Diabetic osteoporosis (DOP) belongs to secondary osteoporosis caused by diabetes; it has the characteristics of high morbidity and high disability. In the present study, we constructed a type 1 diabetic rat model and administered chondroitin sulfate (200 mg/kg) for 10 weeks to observe the preventive effect of chondroitin sulfate on the bone loss of diabetic rats. The results showed that chondroitin sulfate can reduce blood glucose and relieve symptoms of diabetic rats; in addition, it can significantly increase the bone mineral density, improve bone microstructure, and reduce bone marrow adipocyte number in diabetic rats; after 10 weeks of chondroitin sulfate administration, the SOD activity level was upregulated, as well as CAT levels, indicating that chondroitin sulfate can alleviate oxidative stress in diabetic rats.

Emergence of a (M) Variant Conferring Resistance to Tigecycline in .

The aim of this study was to gain insight into the resistance determinants conferring resistance to tigecycline in (.) and to investigate the genetic elements involved in their horizontal transfer. A total of 31 tetracycline-resistant isolates were screened for tigecycline resistance by broth microdilution.

Prevalence and genetic analysis of porcine circovirus type 2 (PCV2) and type 3 (PCV3) between 2018 and 2020 in central China.

Porcine circovirus type 2 (PCV2) is the causative agent of porcine circovirus-associated diseases (PCVAD), causing substantial economic losses to the swine industry worldwide. PCV3, as a recently discovered virus, is associated with porcine dermatitis, nephropathy syndrome, reproductive failure, congenital tremors, and other clinical symptoms. To further investigate the epidemic profile and genetic diversity of the two viruses, a total of 198 samples from swine at various growth stages suspected for PCVAD on 55 different pig farms between 2018 and 2020 were analyzed for presence of PCV2 and PCV3 by using a multiplex real-time PCR assay.

Combined coronary CT angiography with plain scan for diagnosis of ruptured plaque: comparison with optical coherence tomography.

To evaluate the diagnostic efficacy of CCTA + plain scan for ruptured plaques, with optical coherence tomography (OCT) as the reference, and to provide preliminary analysis of influential factors. Patients who underwent CCTA and OCT were retrospectively enrolled. The diagnostic standards for ruptured plaque on CCTA + plain scan were ulcer or intra-plaque dye penetration on CCTA, and a careful review of images from the plain scans to ensure areas of them were not calcification.

Simultaneous detection and differentiation of porcine circovirus 3 and 4 using a SYBR Green І-based duplex quantitative PCR assay.

Porcine circovirus 4 (PCV4) was a novel circovirus identified from diseased pigs in 2019 in Hunan Province, China, and PCV3 and PCV4 co-infection has been reported. In order to detect and differentiate PCV3 and PCV4 simultaneously, the SYBR Green І-based duplex quantitative PCR (qPCR) assay was established in the present study. The two viruses could be easily distinguished by different Tm values: 86.

Seroprevalence investigation and genetic analysis of pseudorabies virus within pig populations in Henan province of China during 2018-2019.

In late 2011, the outbreak of pseudorabies (PR) occurred in Bartha-K61-vaccinated pig farms and spread rapidly to many provinces of China, causing substantial economic losses to the swine industry. A total of 4708 pig serum samples from Henan province during 2018-2019 were collected to screen for the presence of pseudorabies virus (PRV) gE-specific antibodies, and phylogenetic analysis based on the gE gene of PRV was performed. Of the 4708 serum samples tested, 30.

Detection and genetic characteristics of porcine bocavirus in central China.

To investigate the epidemic profile and genetic diversity of porcine bocavirus (PBoV), 281 clinical samples, including 236 intestinal tissue samples and 45 fecal samples were collected from diarrheic piglets on 37 different pig farms in central China, and two SYBR Green I-based quantitative PCR assays were developed to detect PBoV1/2 and PBoV3/4/5, respectively. One hundred forty-eight (52.67%) of the 281 clinical samples were positive for PBoV1/2, 117 (41.

Global distribution, dissemination and overexpression of potent multidrug efflux pump RE-CmeABC in Campylobacter jejuni.

Objectives: To investigate the global distribution, dissemination and overexpression of RE-CmeABC in Campylobacter jejuni.

Methods: WGS information for 433 RE-cmeABC-positive C. jejuni isolates (including 18 isolates sequenced in this study and 415 isolates from GenBank) was used for the generation of minimum-spanning trees with STs.

Studies on the role of IS1216E in the formation and dissemination of poxtA-carrying plasmids in an Enterococcus faecium clade A1 isolate.

Objectives: To analyse the role of IS1216E in the dissemination of the phenicol-oxazolidinone-tetracycline resistance gene poxtA in an Enterococcus faecium clade A1 isolate.

Methods: MICs were determined by broth microdilution. The poxtA-positive isolate was typed by MLST.

Characteristics of the spike and ORF3 genes of porcine epidemic diarrhea virus in Henan and Shanxi provinces of China.

To investigate the epidemic characteristics of porcine epidemic diarrhea virus (PEDV), 135 clinical samples (including intestinal tissues and feces) were collected from diseased piglets during outbreaks of diarrhea from 2015 to 2019 on farms in Henan and Shanxi provinces of China where swine had been immunized with attenuated PEDV (CV777). A total of 86 clinical samples (86/135, 63.7%) were positive for PEDV by RT-PCR, and subsequently, the complete spike (S) and ORF3 genes of 32 PEDV samples were sequenced.

Molecular detection and phylogenetic analysis of Porcine circovirus 4 in Henan and Shanxi Provinces of China.

Porcine circovirus 4 (PCV4), a new circovirus with a distinct relationship to other circoviruses, was identified in 2019 in several pigs with severe clinical disease in Hunan Province, China. To investigate the epidemic profile and genetic diversity of the virus, 63 clinical samples were collected from 24 different pig farms in 14 cities in Henan and Shanxi Provinces, China, between February 2018 and December 2019, and the partial Cap gene of PCV4 was amplified by PCR. Among the 63 samples, 16 (25.

Photocatalytic Remote Oxyfluoroalkylation of Heteroalkynes: Regio-, Stereo-, and Site-Selective Access to Complex Fluoroalkylated ()-Alkenes.

A visible-light-induced remote oxyfluoroalkylation, including ketofluoroalkylation and hydroxytrifluoromethylation, of heteroalkynes is developed with dimethyl sulfoxide (DMSO) and HO as the oxygen source, respectively. It provides a facile access to complex fluoroalkylated ()-alkenes in satisfactory yields with excellent regio-, stereo-, and site-selectivity. The reaction involves an uncommon vinyl radical-induced intermolecular C(sp)-H functionalization, thus offering a good platform for the development of remote difunctionalization of alkynes.

A novel multiresistance gene cluster located on a plasmid-borne transposon in Listeria monocytogenes.

Objectives: To identify the genetic context and the transferability of the multiresistance gene lsa(E) in Listeria monocytogenes.

Methods: MICs were determined by broth microdilution. Transferability of lsa(E) was investigated by conjugation, electrotransformation and natural transformation.

MnO@Corncob Carbon Composite Electrode and All-Solid-State Supercapacitor with Improved Electrochemical Performance.

Two corncob-derived carbon electrode materials mainly composed of micropores (activated carbon, AC) and mesopores/macropores (corncob carbon, CC) were prepared and studied after the anodic electrodeposition of MnO. The capacity of the MnO/activated carbon composite (MnO@AC) electrode did not noticeably increase after MnO electrodeposition, while that of the MnO/corncob carbon composite (MnO@CC) electrode increased up to 9 times reaching 4475 mF cm. An asymmetric all-solid-state supercapacitor (ASC) was fabricated using AC as the anode, MnO@CC as the cathode, and polyvinyl alcohol (PVA)/LiCl gel as the electrolyte.