Cytotoxicity and Bone Biocompatibility of the C-Root SP Experimental Root Canal Sealer.

This study evaluated the cytotoxicity and biocompatibility of a new strontium silicate-based root canal sealer (C-Root SP), in comparison with those of iRoot SP and AH plus. The sealer extract was diluted to the concentrations of 100%, 75%, 50%, and 25%. L929 cells were cultured for 24 h, and the absorbance value was determined.

Corilagin enhances the anti-tumor activity of 5-FU by downregulating the expression of GRP 78.

Colorectal cancer is one of the most common malignancies worldwide. Although initially effective, patients who receive chemotherapy ultimately experience various complications and develop chemo-resistance, leading to cancer recurrence. Therefore, we aimed to find a drug with good efficacy and low toxicity that could enhance the treatment with 5-Fluorouracil (a commonly used clinical drug) and reduce its dosing.

Two new constituents from the endophyte of and its anti-neuroinflammatory activity guided by molecular docking.

Two new compounds, 3-hydroxy-1-(3-hydroxy-5-methoxyphenyl)-2-methyl propan-1-one ( and 1,2,6-trihydroxy-8-methoxy-2,3,3a,9b-tetrahydrocyclopenta[c] isochromen-5(1H)-one ), along with nine known compounds 3-11, involving pyranones, phenylpropenoids and alkaloids, were obtained from , an endophyte isolated from The structures were elucidated by extensive spectroscopic analyses, including 1D NMR, 2D NMR, HRESIMS, IR, UV spectroscopy. The absolute configuration was established spectroscopy techniques and X-ray crystallisation method. Furthermore, guided by molecular docking, compounds 1 and 3 exhibited promising anti-neuroinflammatory activity in LPS-induced BV-2 microglial cells, with IC values of 0.

[In vitro study on cytotoxicity, osteogenic potential and antibacterial activity of silicate root canal sealers].

Purpose: To evaluate the in vitro biocompatibility and antibacterial activity of a new type of strontium silicate-based C-Root SP root canal sealer, and to provide a reference for clinical selection of sealers.

Methods: C-Root SP, iRoot SP and AH Plus extracts were prepared, L929 cells and MC3T3-E1 cells were cultured in vitro, and the cytotoxicity and osteogenic potential of the three sealers were compared. Fresh sealers were mixed with Enterococcus faecalis solution and the antibacterial activity of the sealer was determined by direct contact text (DCT).

Secondary metabolites of : A comprehensive review of chemical diversity and pharmacological properties.

Fungi are considered to be one of the wealthiest sources of bio-metabolites that can be employed for yielding novel biomedical agents. , including parasitic, saprophytic, and endophytic species, is a kind of dark fungi that can produce a broad array of secondary metabolites (SMs) widely distributed in many ecosystems. These are categorized into polyketides, nitrogen-containing compounds, quinones, terpenes, and others based on the unique structural features of the metabolites.

Pharmacy student's perceptions, behaviours and attitudes toward virtual reality simulation.

Aims: The definition of virtual reality simulation (VRS) used for study is the recreation of realistic simulation in a fully online situation with an immersive environment for learning an activity. The study aims to evaluate pharmacy students' perspectives, behavioral and attitude characteristics in the process of VRS course requiring practical skills.

Materials And Methods: This cross-sectional study was based on quantitative questionnaires analysis.

Antiviral Activity of Benzoheterocyclic Compounds from Soil-Derived Streptomyces jiujiangensis NBERC-24992.

Pseudorabies virus (PRV) is a pathogen that causes Aujeszky's disease (AD) in animals, leading to huge economic losses to swine farms. In order to discover anti-PRV compounds, we studied the extracts of the strain NBERC-24992, which showed significant anti-PRV activity. Eight benzoheterocyclic secondary metabolites, including three new compounds (-, virantmycins D-G) and five known compounds (-, virantmycin, A-503451 D, A-503451 D acetylate, A-503451 A, and A-503451 B), were isolated from the broth of NBERC-24992.

Identification of ligustrazine-based analogs of piperlongumine as potential anti-ischemic stroke agents.

Piper longum has a specific aroma and spicy taste. In addition to edible value, current studies have shown that piper longum also has pharmacological activities such as anti-platelet aggregation, anti-inflammation, anti-cancer, anti-diabetes and anti-depression. Piperlongumine is an alkaloid isolated from Piper longum.

A Review of the research methods and progress of biocompatibility evaluation of root canal sealers.

The function of root canal sealer was to achieve an appropriate three-dimensional filling effect by filling the root canal and some irregular lumen, thereby inhibiting the residual bacteria. There were many types of sealers, but research to find the most suitable ones was still ongoing. In recent years, researchers had continuously improved the performance of sealers by developing new sealers or adding active ingredients to the sealers.

Prevalence and correlates of cigarette smoking among Dulong adults in China: A cross-sectional survey in 2020.

Background: The Dulong people are one of the minorities in China with the lowest population. In recent years, the lifestyle of the Dulong people has also changed drastically due to income growth and urbanization. This study aims to identify cigarette smoking prevalence and potential risk factors among Dulong adults in China.

Secondary Metabolites from Marine-Derived : A Comprehensive Review of Origins, Structures, and Bioactivities.

The marine is a highly complex ecosystem including various microorganisms. species is a predominant microbialflora widely distributed in marine ecosystems. This review aims to provide a systematic summary of the newly reported metabolites produced by marine-derived species over recent years covering the literature from 2014 to 2021.

An unprecedented ergostane with a 6/6/5 tricyclic 13(14 → 8)abeo-8,14-seco skeleton from Talaromyces adpressus.

Talasterone A (1), an unprecedented 6/6/5 tricyclic 13(14 → 8)abeo-8,14-seco-ergostane steroid, together with two known congeners dankasterone B (2) and (14β,22E)-9,14-dihydroxyergosta-4,7,22-triene-3,6-dione (3), were characterized from Talaromyces adpressus. The structure of 1 with absolute configuration was elucidated based on NMR spectroscopic data and ECD calculation. Compound 2 belongs to a class of unconventional 13(14 → 8)abeo-ergostanes, which have been renewed via the 1,2-migration of C-13-C-14 bond to C-8.

Enhancement of axial resolution and image contrast of a confocal microscope by a microsphere working in noncontact mode.

A new technique, to the best of our knowledge, for improving the axial resolution and imaging contrast of a reflection mode confocal microscope is proposed. A 50 µm silica microsphere is added in front of the objective lens to enhance both the focusing of illumination and the collection of reflected and scattered light from sample surfaces in noncontact mode. An adjustable pinhole is used to compensate the displacement of the focal point in the axial direction.

Two new dipimprinine alkaloids from soil-derived Streptomyces sp. 44414B.

Two new dipimprinine alkaloids dipimprinine E (1) and dipimprinine F (2) were isolated from Streptomyces sp. 44414B. The structure was elucidated by extensive spectroscopic analysis, including ESI-MS, HR-MS, and 1D and 2D NMR experiments.

Origins, Structures, and Bioactivities of Secondary Metabolites from Marine-derived Penicillium Fungi.

In recent years, marine-derived Penicillium fungi have received remarkable interest as a valuable source of novel natural products encompassing diverse chemical structures and bioactive properties. Mangroves, sediments, algae, and sponges are the four main sources of marine-derived Penicillium fungi. As of 2014, more than 390 novel natural products have been isolated from the marine- derived Penicillium fungi, mainly including polyketides, alkaloids, terpenoids, and macrolides.

Betulin isolated from Pyrola incarnata Fisch. inhibited lipopolysaccharide (LPS)-induced neuroinflammation with the guidance of computer-aided drug design.

This study aims to investigate active phytochemicals isolated from Pyrola incarnata Fisch. (P. incarnata) and their protection against neuroinflammation induced by LPS.

Structurally Diverse Meroterpenoids from a Marine-Derived sp. Fungus.

Three new meroterpenoids, asperaustins A-C (-), and seven known analogues (-) were isolated from a marine-derived sp. fungus. The structures and absolute configurations of these new compounds were unequivocally determined by extensive spectroscopic analyses and single-crystal X-ray diffraction analyses.

Pyrola incarnata demonstrates neuroprotective effects against β-amyloid-induced memory impairment in mice.

This study aims to investigate the neuroprotective effects of Pyrola incarnata against β-amyloid-induced memory impairment in mice. Ethanol extract of Pyrola incarnata (EPI) was obtained and led to eleven phytochemicals successfully by isolation and purification, which were elucidated by spectroscopic analysis (H NMR, C NMR and HR-ESI-MS). Thereinto, ursolic acid was gained as most abundant monomer.

A Comprehensive Review of the Genus Pyrola Herbs in Traditional Uses, Phytochemistry and Pharmacological Activities.

Pyrola (Pyrolaceae), also known as Luxiancao/in China, was recorded in Sheng Nong's Herbal Classic listed in top grade. Pyrola herbs were used as medicinal plants for a long history with wide-ranging activities such as nourishing kidney-yang, strengthening muscles and bones, activating blood, stopping bleeding, dispelling rheumatism, and eliminating dampness. Currently, the research on Pyrola plants is increasing year by year but there is no comprehensive and detailed review concerning genus Pyrola.

A New Macrodiolide and Two New Polycyclic Chromones from the Fungus sp. SCSIO041218.

A new macrodiolide, mangrovlide A () and two new polycyclic chromones, penixanthones C () and D (), as well as four other known compounds (-), have been isolated from the mangrove sediment derived fungus sp. SCSIO041218, cultured in the 0.25% NaCl rice substrate.

The in silico and in vivo evaluation of puerarin against Alzheimer's disease.

The root of Pueraria lobata has been utilized as a food source for thousands of years in China. Puerarin is the major bioactive and the most abundant secondary metabolite obtained from the root of P. lobata.

Spirostaphylotrichin X from a Marine-Derived Fungus as an Anti-influenza Agent Targeting RNA Polymerase PB2.

A new spirocyclic γ-lactam, named spirostaphylotrichin X (1), and three related known spirostaphylotrichins (2-4) were isolated from the marine-derived fungus Cochliobolus lunatus SCSIO41401. Their structures were determined by spectroscopic analyses. Spirostaphylotrichin X (1) displayed obvious inhibitory activities against multiple influenza virus strains, with IC values from 1.

[Elevated expression of CLOCK is associated with poor prognosis in hepatocellular carcinoma].

Objective To evaluate the expression of circadian locomotor output cycles kaput (CLOCK) and its effects on cell growth in hepatocellular carcinoma (HCC). Methods The expression of CLOCK in 158 pairs of human HCC tissues and matched noncancerous samples was detected by immunohistochemical (IHC) staining. The expression of CLOCK in HCC patients was also verified using the data from GEO and TCGA (a total of 356 cases).

Antioxidant activity against HO-induced cytotoxicity of the ethanol extract and compounds from Pyrola decorate leaves.

Context: The leaves of Pyrola decorate H. Andr (Pyrolaceae), known as Luxiancao, have long been used for treating kidney deficiency, gastric haemorrhage and rheumatic arthritic diseases in traditional Chinese medicine.

Objective: The phytochemicals and antioxidant capacities in vitro of P.