Identification of End-Binding 1 Protein as Novel α-4 Giardin-Binding Partners in Giardia lamblia Trophozoites.

Background: Giardia lamblia (syn. G. intestinalis, G.

Correlation of Serum IL-17, VEGF, and Lactate Dehydrogenase (LDH) Levels with Prognosis of Gastric Cancer.

Objective: To investigate the correlation of serum interleukin-17 (IL-17), vascular endothelial growth factor (VEGF) and lactate dehydrogenase (LDH) levels with the prognosis of gastric cancer patients.

Methods: From December 2018 to December 2020, 45 patients with gastric cancer treated in our hospital and 50 healthy individuals were assessed for eligibility and recruited. The eligible patients were assigned to an observation group, and the healthy subjects were assigned to a control group.

Establishment of a Lung Cancer Discriminative Model Based on an Optimized Support Vector Machine Algorithm and Study of Key Targets of Wogonin in Lung Cancer.

An optimized support vector machine model was used to construct a lung cancer diagnosis model based on serological indicators, and a molecular regulation model of Wogonin, a component of , was established. Serological indexes of patients were collected, the grid search method was used to identify the optimal penalty coefficient C and parameter g of the support vector machine model, and the benign and malignant auxiliary diagnosis model of isolated pulmonary nodules based on serological indicators was established. The regulatory network and key targets of Wogonin in lung cancer were analyzed by network pharmacology, and key targets were detected by western blot.

[Establishment of dual reverse phase chromatography combined parallel reaction monitoring for targeted quantitative proteomics].

Steady improvement in mass spectrometers technology has transformed the targeted proteome analysis into a new stage. Parallel reaction monitoring (PRM) technology has evolved from the basic multiple reaction monitoring (MRM) targeted proteomics methods in recent years. PRM performs with a higher sensitivity, throughput and reproducibility in targeted quantification, however its limitations in effectiveness and accurate quantification of samples with higher complexity still remain unsolved.

The mediating role of emotional intelligence between negative life events and psychological distress among nursing students: A cross-sectional study.

Background: Previous studies have highlighted that negative life events and emotional intelligence are significant predictors of mental health. However, whether emotional intelligence mediates the relationship between negative life events and psychological distress among nursing students have not been given adequate attention.

Objectives: To explore the relationship among negative life events, emotional intelligence and psychological distress and to examine the mediating role of emotional intelligence in psychological distress among Chinese nursing students.

Co-circulation of Hantaan, Kenkeme, and Khabarovsk Hantaviruses in Bolshoy Ussuriysky Island, China.

Hemorrhagic fever with renal syndrome (HFRS) was first recognized in far eastern Asia in the 1930s, and has been highly prevalent in this region ever since. To reveal the molecular epidemiology of hantaviruses in this region, a total of 374 small mammals (eight species of rodents and one species of shrew) were captured in the Chinese part of the Bolshoy Ussuriysky Island (Heilongjiang Province). Hantavirus sequences were recovered from three striped field mice (Apodemus agrarius), 11 Maximowicz's voles (Microtus maximowiczii), and one flat-skulled shrew (Sorex roboratus).

[Changes of IFN-gamma, IL-4 and T cells in Schistosoma japonicum-infected mice after praziquantel treatment].

Objective: To investigate the serum levels of IFN-gamma and IL-4, and the dynamic changes of IFN-gamma-specific and IL-4-specific lymphocytes in mice with Schistosoma japonicum infection after treatment by praziquantel.

Methods: Ninety BALB/c mice were randomly divided into three groups (n = 30) named as infection group, treatment group and control group. The mice in treatment group and infection group were infected with (25 +/- 2) S.

Construction and characterization of a full-lengh cDNA library from non-fresh Giardia lamblia.

Objective: To construct rapidly a full-length cDNA library from nanogram amounts total RNA of Giardia lamblia (G. lamblia) trophozoites stocked in RNA stabilization reagent.

Methods: Total RNA of Giardia was extracted using Trizol reagent.

Toll-like receptor 5 agonism protects mice from radiation pneumonitis and pulmonary fibrosis.

Radiation pneumonitis and pulmonary fibrosis are the main complications with radiotherapy for thoracic neoplasms, directly limiting the efficient dose in clinical application and currently there are few medicines that effectively function as radioprotectants. However, a TLR5 agonist, CBLB502, was confirmed to have protective efficacy against hematopoietic and gastrointestinal radiation syndromes in mice and primates. This study points to a new direction for protection against thoracic radiation-induced pulmonary syndromes and skin injury by CBLB502.

[Construction of GCV-specific hammerhead ribozyme recombinant vector of alpha-8 giardin in Giardia lamblia].

Objective: To construct a GCV-ribozyme recombinant vectors of alpha-8 giardin in Giardia lamblia.

Methods: The secondary structure of alpha-8 giardin mRNA (GenBank Accession No. AY781323) was analyzed with the RNA draw software.

[Facial Demodex infection among college students in Tangshan].

A survey indicated that the prevalence of Demodex infection among 512 college students in Tangshan was 36.3% (186/512), that of males and females was 39.3% (81/206) and 34.

[Entamoeba gingivalis infection among college students in Tangshan].

Objective: To investigate the prevalence of Entamoeba gingivalis infection in college students in Tangshan, and analyze the relationship between the infection and human behaviors.

Methods: 551 students of grades 1-3 from six colleges in Tangshan received questionnairing, which covered the oral health state, teeth-brushing, xylitol gum-chewing, diet fondness, and smoking. Specimens were taken from the tooth surface of the lesion or fouling materials by using disinfected toothpicks and the smears were observed microscopically to examine Entamoeba gingivalis infection.

[The injury of metronidazole on morphology of Giardia lamblia in vitro].

Trophozoites of Giardia lamblia were axenically cultivated with modified TYI-S-33 medium contained 500 microg/ml metronidazole (12h LC50). The morphology of drug-treated trophozoites was observed with light and electron micro-scopes at 2, 4, 8, 12 h respectively. The light microscopy revealed that the trophozoites treated with MTZ showed swollen, detached from the wall of the culture tube, and were with vacuoles in the cytoplasm.

[Human parasitology teaching in the 21st century].

Multimedia techniques were applied in parasitological teaching and experimental practices. In order to strengthen the practical ability of the undergraduate students, reform was conducted including a prioritization of the teaching content, use of series micro-slides and video show, etc.

[Effect of dihydroartemisinin on ultrastructure of Giardia lamblia in vitro].

Objective: To study the in vitro effect of dihydroartemisinin (DHA) on Giardia lamblia.

Methods: Trophozoites of G. lamblia were cultivated with modified TYI-S-33 medium that contains dihydroartemisinin (DHA).