Publications by authors named "Xieyang Guo"
Article Synopsis
- RNA polymerase (RNAP) pauses during DNA transcription to help regulate gene expression, with the transcription factors NusA and NusG influencing this pausing in opposing ways.
- Cryo-EM studies of Escherichia coli RNAP show how NusA promotes RNAP's swivelling, leading to more pausing, while NusG reduces this effect.
- The findings suggest that NusA and NusG work together during transcription termination, providing insights into the randomness of pausing and how these factors modulate the process.
Bromodomain-containing protein 4 (BRD4) is an epigenetic reader and oncology drug target that regulates gene transcription through binding to acetylated chromatin via bromodomains. Phosphorylation by casein kinase II (CK2) regulates BRD4 function, is necessary for active transcription and is involved in resistance to BRD4 drug inhibition in triple-negative breast cancer. Here, we provide the first biophysical analysis of BRD4 phospho-regulation.
View Article and Find Full Text PDFRegulatory sequences or erroneous incorporations during DNA transcription cause RNA polymerase backtracking and inactivation in all kingdoms of life. Reactivation requires RNA transcript cleavage. Essential transcription factors (GreA and GreB, or TFIIS) accelerate this reaction.
View Article and Find Full Text PDFTranscriptional pausing by RNA polymerases (RNAPs) is a key mechanism to regulate gene expression in all kingdoms of life and is a prerequisite for transcription termination. The essential bacterial transcription factor NusA stimulates both pausing and termination of transcription, thus playing a central role. Here, we report single-particle electron cryo-microscopy reconstructions of NusA bound to paused E.
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