Publications by authors named "Xie-Chao He"
Article Synopsis
- * Researchers identified a gene called OOEP as a crucial player in the homologous recombination (HR) DNA repair mechanism specifically in mouse oocytes, which is essential for repairing DSBs.
- * OOEP influences the effectiveness of DNA repair by activating the ATM kinase and allowing the accumulation of Rad51 at DSB sites, and its reduced expression with maternal age may contribute to declining oocyte quality.
Study Hypothesis: What factors in mouse oocytes are involved in the ageing-related decline in oocyte quality?
Study Finding: The maternal effect gene Mater is involved in ageing-related oocyte quality decline in mice.
What Is Known Already: Premature loss of centromere cohesion is a hallmark of ageing-related oocyte quality decline; the maternal effect gene Mater (maternal antigen that embryos require, also known as Nlrp5) is required for preimplantation embryo development beyond the 2-cell stage, and mRNA expression of Mater decreases with maternal ageing.
Study Design, Samples/materials, Methods: Mater protein expression level in mature oocytes from 7 young (5-8 weeks old) to 7 old mice (41-68 weeks old) was compared by immunoblotting analysis.
Floped (official name Ooep) is specifically and abundantly expressed in mouse oocytes and embryonic stem cells (ESCs). Depletion of Floped from oocytes leads to early embryonic arrest at the 2-cell stage. Although crucial in cleavage stage development, its roles in early embryos as well as in ESCs remain completely unknown.
View Article and Find Full Text PDFThe PI3K/Akt signal transduction pathway plays an important role in pre-implantation embryonic development. The tumor suppressor gene PTEN negatively regulates the PI3K/Akt pathway. Although PI3K is constitutively activated during pre-implantation embryonic development, currently no evidence shows the presence and possible involvement of PTEN in early embryo development.
View Article and Find Full Text PDFAim: To establish a method for cynomolgus monkey sperm cryopreservation in a chemically defined extender.
Methods: Semen samples were collected by electro-ejaculation from four sexually mature male cynomolgus monkeys. The spermatozoa were frozen in straws by liquid nitrogen vapor using egg-yolk-free Tes-Tris mTTE synthetic extender and glycerol as cryoprotectant.