Warifteine Alleviates ALI and Inhibits NETosis by Decreasing ROS-induced GSDMD Oligomerization.

Acute lung injury (ALI) is characterized by exaggerated inflammatory reactions and high clinical mortality rates, but targeted therapeutic interventions are lacking. Warifteine, which is a traditional remedy known for its anti-inflammatory properties, has been identified, but its potential effects on ALI remain unexplored. In this study, a murine model of ALI was established by injecting lipopolysaccharide (LPS) into wild-type or neutrophil-specific Gasdermin D (Gsdmd)-deficient mice.

Flow Cytometry for Assessing Human Sperm Acrosomal Reaction (AR) and Correlation of AR and Acrosomal Enzyme Activity With Semen Parameters.

Background/aim: This study aimed to evaluate human sperm acrosomal reaction (AR) using flow cytometry and examine the correlation of AR and acrosomal enzyme activity (AEA) with seven semen parameters: total sperm count, concentration, vitality, motility, morphology, survival rate, and sperm DNA fragmentation index (DFI).

Patients And Methods: A retrospective analysis was conducted on semen data from 398 men in the AR group and 526 men in the AEA group. AR was assessed using flow cytometry, while AEA was measured with the solid-phase BAPNA method.

Utilizing High-Resolution Mass Spectrometry Data Mining Strategy in R Programming Language for Rapid Annotation of Absorbed Prototypes and Metabolites of Gypenosides.

The rapid and accurate annotation of the complex compounds and metabolites in natural products remains a significant challenge. In this study, we developed an integrated strategy to efficiently and accurately profile both the prototypes and metabolites of natural products in vivo. And this was achieved by establishing a gypenosides constituent database and utilizing R programming language to combine sample selection, virtual metabolite database construction, polygon mass defect filtering, and Kendrick mass defect filtering.

Gαq/11 aggravates acute lung injury in mice by promoting endoplasmic reticulum stress-mediated NETosis.

Background: Acute lung injury (ALI) is distinguished by exaggerated neutrophil extracellular traps (NETs), elevated clinical mortality rates, and a paucity of targeted therapeutic interventions. The Gαq/11 protein, a member of the G protein subfamily, is an effective intervention target for a variety of diseases, but little is known about its role in ALI.

Methods: In this study, a murine model of ALI induced by lipopolysaccharide (LPS) was utilized, employing myeloid cell-specific Gna11 knockout mice.

Lactobacillus vaginalis alleviates DSS induced colitis by regulating the gut microbiota and increasing the production of 3-indoleacrylic acid.

Ulcerative colitis (UC) is a chronic inflammatory disorder, and its incidence is experiencing an upward trend worldwide. UC can result in gut microbiota dysbiosis, impaired intestinal epithelial barrier, and systemic inflammation, for all of which there is presently no definitive treatment available. Lactobacillus is known to regulate gut microbiota and related metabolites to intervene in the development of UC.

Highly Sensitive and Selective Detection of Uranyl Ions Based on a Tb-Functionalized MOF via Competitive Host-Guest Coordination.

Owing to the rapid development of the nuclear industry, uranium has become a global environmental contaminant due to its radiotoxicity and chemotoxicity, posing significant threats to human health and ecological safety. Although various instrumental and chemical analytical methods have been developed for uranyl ion detection in aquatic environments, searching for new sensors with high stability, sensitivity, and selectivity remains a challenge. In this study, a luminescent Zr-based metal-organic framework (MOF), designated as Tb@UiO-66-(COOH), was successfully synthesized utilizing a postsynthetic exchange (PSE) method along with Tb ion doping for uranyl ion detection.

Spatial Distribution and Dietary Risk Assessment of Aflatoxins in Raw Milk and Dairy Feedstuff Samples from Different Climate Zones in China.

This study aimed to explore the contamination of aflatoxins by investigating the spatial distribution of aflatoxin B1 (AFB1) in cow feedstuff and aflatoxin M1 (AFM1) in raw milk, and the potential health risks of AFM1 in milk and dairy products. Feedstuff and raw milk were collected from 160 pastures in three climate zones of China from October to November 2020. The results indicated the level of AFB1 and AFM1 ranged from 51.

Clinical efficacy and safety of vortioxetine as an adjuvant drug for patients with bipolar depression.

Objectives: Whether vortioxetine has a utility as an adjuvant drug in the treatment of bipolar depression remains controversial. This study aimed to validate the efficacy and safety of vortioxetine in bipolar depression.

Methods: Patients with bipolar Ⅱ depression were enrolled in this prospective, two-center, randomized, 12-week pilot trial.

Androgen deficiency-induced loss of Lactobacillus salivarius extracellular vesicles is associated with the pathogenesis of osteoporosis.

Male osteoporosis is primarily caused by a decrease in testicular testosterone production. Male osteoporosis remains a disease with insufficient diagnosis and treatment, and its consequences are severe, especially in older patients. The gut microbiota plays a crucial role in its occurrence and development.

A Constant Filgotinib Delivery Adhesive Platform Based on Polyethylene Glycol (PEG) Hydrogel for Accelerating Wound Healing via Restoring Macrophage Mitochondrial Homeostasis.

Skin wound healing is often hindered by disrupted mitochondrial homeostasis and imbalanced macrophage glucose metabolism, posing a critical challenge to improve patient outcomes. Developing new wound healing dressings capable of effectively regulating macrophage immune-metabolic functions remains a pressing issue. Herein, a highly adhesive polyethylene glycol (PEG) hydrogel loaded with the Janus kinase 1 (JAK1) inhibitor Filgotinib (Fil@GEL) is prepared to modulate macrophage metabolic reprogramming and restore normal mitochondrial function.

Analysis of influencing factors and construction of nomogram prediction model of postoperative kinesiophobia in patients undergoing total knee arthroplasty.

Objective: To explore the influencing factors of kinesiophobia and to establish a nomogram prediction model.

Methods: A retrospective observational study was conducted in 354 patients who underwent TKA in Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine from December 2019 to December 2022. Sociodemographic characteristics, anesthesia methods, Tampa Scale for Kinesiophobia (TSK), numeric rating scale (NRS), hospital for special surgery (HSS), activities of daily living (ADL), and self-rating anxiety scale (SAS) were reviewed and analyzed.

Ultra-Fast Degradation of Mustard Gas Simulant by Titanium Dioxide-Phosphomolybdic Acid Sub-1 nm Nanobelts.

The development of novel catalysts for the rapid detoxification of sulfur mustard holds paramount importance in the field of military defense. In this work, titanium dioxide-phosphomolybdic acid sub-1 nm nanobelts (TiO/PMA SNBs) are employed as effective catalysts for the ultra-fast degradation of mustard gas simulants (2-chloroethyl ethyl sulfide, CEES) with 100% selectivity and a half-life (t, time required for 50% conversion) as short as 12 s, which is the fastest time to the best of the knowledge. Even in dark conditions, this material can still achieve over 90% conversion within 5 min.

Molecular characteristics of dissolved organic matter regulate the binding and migration of tungsten in porous media.

Tungsten (W) is an emerging contaminant that poses potential risks to both the environment and human health. While dissolved organic matter (DOM) can significantly influence the W's environmental behavior in natural aquifers, the mechanisms by which DOM's molecular structure and functional group diversity impact W binding and migration remain unclear. Using molecular weight-fractionated soil and sediment DOM (<1 kDa, 1-100 kDa, and 100 kDa-0.

Blood-brain barrier damage accelerates the accumulation of micro- and nanoplastics in the human central nervous system.

The widespread use of plastics has led to increased micro- and nanoplastics (MNPs) pollution, resulting in significant environmental challenges and concerns about potential harm to human health. This study investigated whether certain types of MNPs can accumulate in the human central nervous system (CNS) and trigger inflammatory responses, particularly after CNS infection. Our analysis of 28 cerebrospinal fluid (CSF) samples from 28 patients with or without CNS infection revealed that only polystyrene (PS), polyethylene (PE), polypropylene (PP), and polyvinyl chloride (PVC) were capable of selectively entering the human CNS.

Exploring the neural link between childhood maltreatment and depression: a default mode network rs-fMRI study.

Background: Childhood maltreatment (CM) is increasingly recognized as a significant risk factor for major depressive disorder (MDD), yet the neural mechanisms underlying the connection between CM and depression are not fully understood. This study aims to deepen our understanding of this relationship through neuroimaging, exploring how CM correlates with depression.

Methods: The study included 56 MDD patients (33 with CM experiences and 23 without) and 23 healthy controls.

A Self-Constructed Mg/K Co-Doped Prussian Blue with Superior Cycling Stability Enabled by Enhanced Coulombic Attraction.

Prussian blue (PB) is regarded as a promising cathode for sodium-ion batteries because of its sustainable precursor elements (e.g., Mn, Fe), easy preparation, and unique framework structure.

Comprehensive analysis of hypoxia-related genes in diagnosis and immune infiltration in acute myocardial infarction: based on bulk and single-cell RNA sequencing data.

Background: Hypoxia has been found to cause cellular dysfunction and cell death, which are essential mechanisms in the development of acute myocardial infarction (AMI). However, the impact of hypoxia-related genes (HRGs) on AMI remains uncertain.

Methods: The training dataset GSE66360, validation dataset GSE48060, and scRNA dataset GSE163956 were downloaded from the GEO database.

Multifunctional human serum albumin-crosslinked and self-assembling nanoparticles for therapy of periodontitis by anti-oxidation, anti-inflammation and osteogenesis.

Periodontitis is a chronic inflammatory disease that can result in the irreversible loss of tooth-supporting tissues and elevate the likelihood and intensity of systemic diseases. The presence of reactive oxygen species (ROS) and associated related oxidative stress is intricately linked to the progression and severity of periodontal inflammation. Targeted removal of local ROS may serve to attenuate inflammation, improve the unfavorable periodontal microenvironment and potentially reverse ensuing pathological cascades.

[Nutritional components of 5 potatoes in Anqing City in 2021].

Objective: To analyze the nutrient composition and nutritional value evaluation of 5 potatoes in Anqing City.

Methods: According to the requirements of the Technical Manual for Food Composition Monitoring Projects, 5 types of potato samples were collected from Anqing City, Anhui Province. National standard detection method were used to determine the nutritional components such as water, ash, protein, fat, dietary fiber, sugar, minerals, vitamins, and amino acids in the samples.

Metabolomic insights into -fermented rice products: Implications for monacolin K content and nutritional optimization.

This study aims to elucidate the detailed metabolic implications of varying monacolin K levels and sterilization methods on -fermented rice products (MFRPs), acclaimed for their health benefits and monacolin K content. Advanced metabolite profiling of various MFRP variants was conducted using ultrahigh-performance liquid chromatography coupled with tandem time-of-flight mass spectrometry (UHPLC-Q-TOF MS). Statistical analysis encompassed -tests, ANOVA, and multivariate techniques including PCA, PLS-DA, and OPLS-DA.

Connectomic insights into the impact of 1p/19q co-deletion in dominant hemisphere insular glioma patients.

Objectives: This study aimed to elucidate the influences of 1p/19q co-deletion on structural connectivity alterations in patients with dominant hemisphere insular diffuse gliomas.

Methods: We incorporated 32 cases of left insular gliomas and 20 healthy controls for this study. Using diffusion MRI, we applied correlational tractography, differential tractography, and graph theoretical analysis to explore the potential connectivity associated with 1p/19q co-deletion.

Photocatalytic Hydrogen Peroxide Production by a Mixed Ligand-Functionalized Uranyl-Organic Framework.

Hydrogen peroxide (HO) production driven by solar energy has received enormous attention due to its high efficiency, low cost, and environmental friendliness characteristics. Searching for new photocatalytic materials for HO production is one of the most important targets. In this work, a new three-dimensional (3D) uranyl-organic framework material was constructed with mixed ligands via a solvothermal reaction and used for photocatalytic HO production.