Biosensors with vancomycin and polymetallic metal-organic frameworks for colorimetric-fluorescent dual-mode detection and sterilization of bacteria.

The development of a versatile platform for bacterial assay and elimination is urgently needed due to the danger that bacteria pose to human life. Here, we synthesized a trimetallic deposition and horseradish peroxidase (HRP)-embedded porous coordination network-224 hybrid nanozymes (PCN-224 @AuPdPt@HRP) with outstanding peroxidase activity and fluorescence quenching ability. On this basis, we designed a dual recognition strategy-driven colorimetric-fluorescence dual-mode detection platform using Listeria monocytogenes as a pattern analyte.

Nanozyme-catalyzed and zwitterion-modified swabs based for the detection of Listeria monocytogenes in complex matrices.

In recent years, nanozymes have been widely used in the field of biosensing and food safety testing due to their advantages of low cost, high stability, easy modification and adjustable catalytic activity. However, how to reduce the signal interference generated by reducing substances, macromolecules and colored substances in the food matrix in nanozymes-based colorimetric sensing is still a major challenge. In this paper, using Listeria monocytogenes as a model analyte, sodium sulfonyl methacrylate (SBMA) polymers were modified onto cotton swabs by photothermal polymerization and combined with Listeria monocytogenes-specific aptamer (Apt1) to prepare swabs that can specifically capture and isolate Listeria monocytogenes from complex matrices (SBMA/Apt1 cotton swab).

Visual Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid On-Site Detection of in Milk Products.

(1) Background: Rapid on-site testing is an effective method for the detection of () in food ingredients and the environment. (2) Methods: In this study, we developed colorimetric loop-mediated isothermal amplification (LAMP) and immunochromatographic test strips (ICTs) for the rapid and visual detection of . This study designed new specific LAMP primers for virulence island genes.

Dihydroxy Polyethylene Additives for Compatibilization and Mechanical Recycling of Polyethylene Terephthalate/Polyethylene Mixed Plastic Waste.

Polymer blend compatibilization is an attractive solution for mechanical recycling of mixed plastic waste because it can result in tough blends. In this work, hydroxy-telechelic polyethylene (HOPEOH) reactive additives were used to compatibilize blends of polyethylene terephthalate (PET) and linear low-density polyethylene (LLDPE). HOPEOH additives were synthesized with molar masses of 1-20 kg/mol by ring-opening metathesis polymerization of cyclooctene followed by catalytic hydrogenation.

Linear, Graft, and Beyond: Multiblock Copolymers as Next-Generation Compatibilizers.

Properly addressing the global issue of unsustainable plastic waste generation and accumulation will require a confluence of technological breakthroughs on various fronts. Mechanical recycling of plastic waste into polymer blends is one method expected to contribute to a solution. Due to phase separation of individual components, mechanical recycling of mixed polymer waste streams generally results in an unsuitable material with substantially reduced performance.

Defining the Macromolecules of Tomorrow through Synergistic Sustainable Polymer Research.

Transforming how plastics are made, unmade, and remade through innovative research and diverse partnerships that together foster environmental stewardship is critically important to a sustainable future. Designing, preparing, and implementing polymers derived from renewable resources for a wide range of advanced applications that promote future economic development, energy efficiency, and environmental sustainability are all central to these efforts. In this contribution, we take a comprehensive, integrated approach to summarize important and impactful contributions to this broad research arena.

Blend Miscibility of Poly(ethylene terephthalate) and Aromatic Polyesters from Salicylic Acid.

Poly(ethylene terephthalate) (PET) is one of the most prevalent polymers in the world due to its combined thermal, mechanical, and gas barrier attributes. Blending PET with other polymers is an appealing strategy to further tailor properties to meet the needs of an even more diverse range of applications. Most blends with PET are macrophase-separated; only a few miscible systems have been reported.

Compatibilization of PP/HDPE Blends with PE--PP Graft Copolymers.

The compatibilization of polyethylene (PE) and isotactic polypropylene (PP) blends is of particular interest due to the challenges associated with recycling these plastics from mixed waste streams. Polyethylene--PP copolymers (PE--PP) were prepared using a grafting-through strategy by copolymerization of ethylene with allyl-terminated PP macromonomers in the presence of a hafnium pyridylamido catalyst. Graft copolymers with a variety of graft lengths ( = 6-28 kg/mol), graft numbers, and graft spacings were prepared.

Multiblock Copolymers for Recycling Polyethylene-Poly(ethylene terephthalate) Mixed Waste.

Plastic pollution is one of the most pressing global environmental issues we face today, in part due to the continued rise in production and use of disposable plastic products. Polyolefins and polyesters are two of the most prevalent polymers in the world accounting for ∼80% of total nonfiber plastic production. Recycling, despite being intrinsically environmentally friendly and sometimes economically viable, remains at a surprisingly low level (<9% in the U.

Recent advances and progress in the detection of bisphenol A.

Bisphenol A (BPA) is an important industrial chemical used as a plasticizer in polycarbonate and epoxy resins in the plastic and paper industries. Because of its estrogenic properties, BPA has attracted increasing attention from many researchers. This review focuses primarily on analytical methods for BPA detection that have emerged in recent years.

[Effect of ascorbic acid, epidermal growth factor and follicle stimulating hormone on in vitro culture of sheep ovarian cortical tissue].

In this study, we evaluated the effects of ascorbic acid (VC), epidermal growth factor (EGF) and follicle stimulating hormone (FSH) on in vitro culture of sheep ovarian cortical tissue. Using 2 x 2 x 2 factor experimental design, we cultured sheep ovarian cortex fragments in 8 media with MEM (control), MEM+VC (50 microg/mL), MEM +EGF (100 ng/mL), MEM+FSH (50 ng/mL), MEM+VC+EGF, MEM+VC+FSH, MEM+EGF+FSH, MEM+VC+EGF+FSH. After 0 (non-cultured control), 2, 6, 12 days of culture, the pieces of ovarian cortex were proceed to histological and proliferating cell nuclear antigen (PCNA) examination, or observed by transmission electron microscopy (TEM).

In vitro culture of sheep lamb ovarian cortical tissue in a sequential culture medium.

Purpose: This study evaluates the effect of a sequential culture system on the follicular development of sheep lamb ovaries, aiming to establish an available in vitro culture system for ovarian culture.

Methods: Lamb ovarian cortical fragments were cultured on a steel mesh with a nitrocellulose membrane pre-coated by type 1 collagen. Several culture media were used for the determinations, specifically, a control medium (alpha-MEM), a constant medium (control medium supplemented with 75 ng/mL human recombinant EGF, 200 mIU/mL sheep FSH, 100 ng/mL human recombinant GDF-9, and 100 ng/mL human recombinant bFGF), and a sequential medium (control medium supplemented with sequential growth factors added on different days).