Visualization and characterization of inflammatory cell recruitment and migration through the corneal stroma in endotoxin-induced keratitis.

Purpose: The infiltration of inflammatory cells into the cornea is a major determinant in the outcome of keratitis. The purpose of this study was to use enhanced green fluorescence protein (EGFP) bone marrow chimeric mice to visualize and characterize the inflammatory cells that migrate to the corneal stroma during endotoxin-induced keratitis and to explore the mechanisms underlying this process.

Methods: Keratitis was induced by injecting endotoxin into the corneas of EGFP chimeric mice.