A novel high sensitive, specificity duplex enzyme-activated differentiating probes PCR method for the SNP detection and differentiation of MS-H vaccine strains from wild-type Mycoplasma synoviae strains.

Mycoplasma synoviae (MS) is a contagious pathogen that poses a significant threat to the poultry industry. Detection plays an important role in the prevention and control of MS, particularly in differentiating between wild-type MS and live attenuated vaccine strains for vaccination selection and culling of animals with wild-type only. The live attenuated ts+ vaccine strain MS-H is recognized as the most effective and widely used vaccine.

Enhanced Diagnostic Efficiency of Endometrial Carcinogenesis and Progression in Women with Abnormal Uterine Bleeding through Peripheral Blood Cytokine Testing: A Multicenter Retrospective Cohort Study.

This study aimed to evaluate the role of plasma cytokine detection in endometrial cancer screening and tumor progression assessment in patients with abnormal uterine bleeding. In this multicenter retrospective cohort study of 287 patients with abnormal uterine bleeding, comprehensive clinical information and laboratory assessments, including cytokines, routine blood tests, and tumor markers, were performed. Associations between the clinical indicators and endometrial carcinogenesis/progression were evaluated.

A CNN-LSTM based deep learning model with high accuracy and robustness for carbon price forecasting: A case of Shenzhen's carbon market in China.

Accurately predicting carbon trading prices using deep learning models can help enterprises understand the operational mechanisms and regulations of the carbon market. This is crucial for expanding the industries covered by the carbon market and ensuring its stable and healthy development. To ensure the accuracy and reliability of the predictions in practical applications, it is important to evaluate the model's robustness.

Prediction of nitrous oxide emission of a municipal wastewater treatment plant using LSTM-based deep learning models.

Accurate assessment of greenhouse gas emissions from wastewater treatment plants is crucial for mitigating climate change. NO is a potent greenhouse gas that is emitted from wastewater treatment plants during the biological denitrification process. In this study, we developed and evaluated deep learning models for predicting NO emissions from a WWTP in Switzerland.

Facilitators and barriers of attending BaDuanJin experienced by breast cancer survivors during chemotherapy.

Purpose: This study aimed to identify facilitators and barriers to adherence to a Baduanjin program experienced by breast cancer survivors (BCSs) during chemotherapy.

Methods: Semi-structured interviews with 10 BCSs in an interprofessional BaDuanJin program were conducted to understand their experiences, including the facilitators and barriers concerning exercise. Content analysis was performed following the behaviour change wheel (BCW).

Life cycle cost analysis of solar energy via environmental externality monetization.

Evaluating the embodied environmental impact of solar photovoltaic (PV) technology has been an important topic in addressing the sustainable development of renewable energy. While monetization of environmental externality is a remaining issue, which should be carried out in order to allow for an easy-to-understand comparison between direct economic and external cost. In this study, the environmental impact of solar PV power is monetized through conversion factors between midpoint and endpoint categories of life cycle analysis and the monetization weighting factor.

Evidences for the mechanism of Shenmai injection antagonizing doxorubicin-induced cardiotoxicity.

Background: Doxorubicin (DOX) is a widely used antitumor drug. However, its clinical application is limited for its serious cardiotoxicity. The mechanism of DOX-induced cardiotoxicity is attributed to the increasing of cell stress in cardiomyocytes, then following autophagic and apoptotic responses.

Multi-watt, broadband second-harmonic-generation in MgO:PPSLT waveguides fabricated with femtosecond laser micromachining.

We demonstrate optical waveguides fabricated in periodically poled MgO-doped stoichiometric lithium tantalate crystals using an fs-laser direct-write process. Two different waveguide architectures were developed: depressed cladding and stress-induced waveguides. Our strain-optic simulations confirmed the guiding mechanism for either case.

High-energy sub-phonon lifetime pulse compression by stimulated Brillouin scattering in liquids.

Multi-Joule level stimulated Brillouin scattering (SBS) pulse compression below the acoustic phonon lifetime is demonstrated with a energy-scalable generator-amplifier setup. Single-pass compression of pulses longer than 20τB (τB as phonon lifetime) to as short as 0.5τB with ~100 mJ pulse energy is realized from the generator, by choosing the focusing length to match approximately with the full length at half maximum of the input Gaussian pulses.

Simultaneous determination and pharmacokinetics of eight ginsenosides by LC-MS/MS after intravenously infusion of 'SHENMAI' injection in dogs.

SHENMAI injection, a prescription comprised of Panax ginseng and Ophiopogon japonicas, is being extensively applied in the field of cardio-protection and immune-modulation in China. Ginsenosides are the main active components in SHENMAI injection. In order to capture and analyze the pharmacokinetic profile of major ginsenosides of SHENMAI injection in Beagle dogs, liquid chromatography equipped with electro-spray ionization and tandem mass spectrometry method was applied in simultaneous determination for protopanaxatriol type ginsenoside (Re, Rf, Rg1), protopanaxadiol type ginsenoside (Rb2, Rb1, Rd, Rc) and oleanolic acid type ginsenoside (Ro).

A Unique Pattern of HCV Genotype Distribution on Hainan Island in China Revealed by Evolutionary Analysis.

Background/aims: Different genotypes of HCV may differ in both disease progression and response to antiviral therapies. Hainan Island has been inhabited by the "Li" aboriginal minority for centuries. We aimed to provide a better understanding of HCV infection on Hainan Island, so that the information would help improve strategies for HCV prevention and control on the island and in the wider country.

Exosome-derived microRNAs contribute to prostate cancer chemoresistance.

Certain microRNAs (miRNAs) play a key role in cancer cell chemoresistance. However, the pleiotropic functions of exosome-derived miRNAs on developing chemoresistance remain unknown. In the present study, we aimed to construct potential networks of miRNAs, which derived from the exosome of chemoresistant prostate cancer (PCa) cells, with their known target genes using miRNA expression profiling and bioinformatic tools.

Polysaccharide Extracted from Laminaria japonica Delays Intrinsic Skin Aging in Mice.

This study aimed to determine the effect of topically applied Laminaria polysaccharide (LP) on skin aging. We applied ointment containing LP (10, 25, and 50 μg/g) or vitamin E (10 μg/g) to the dorsal skin of aging mice for 12 months and young control mice for 4 weeks. Electron microscopy analysis of skin samples revealed that LP increased dermal thickness and skin collagen content.

Spatio-temporal characterization of pulses obtained from a high-energy sub-nanosecond laser system.

Spatio-temporal profiles of laser pulses, obtained from each stage of a high-energy sub-nanosecond laser system, are investigated. The laser system is composed of a Q-switched Nd:YAG unstable oscillator, a chain of Nd:YAG amplifiers, a second-harmonic generator, and a high-energy pulse compressor based on stimulated Brillouin scattering (SBS). A curved energy front, i.

Hepatoma cell-derived leptin downregulates the immunosuppressive function of regulatory T-cells to enhance the anti-tumor activity of CD8+ T-cells.

The adaptive immune response against hepatocellular carcinoma (HCC) could be a therapeutic target to restrain HCC initiation and growth. The interactions between hepatoma cells and immune cells modify the anti-tumor immunity to influence hepatoma cell survival. To explore the potential interplay between hepatoma cells and anti-HCC T-cells, we conducted a HCC induction mouse model to analyze the phenotypic and functional alterations of T-cell subsets.

Diagnostic value of serum Golgi protein 73 for HBV-related primary hepatic carcinoma.

Background: Alpha-fetoprotein (AFP) levels are routinely used for diagnosis and monitoring of hepatic diseases, but it has a limited value. Golgi protein 73 (GP73) has been suggested as a new marker for hepatic diseases.

Objective: To explore the clinical value of serum GP73 in different diseases associated with hepatitis B virus (HBV) infection.

Ring-shaped backward stimulated Raman scattering driven by stimulated Brillouin scattering.

Backward stimulated Raman scattering is generated in water, pumped by pre-compressed pulses from a single-cell stimulated Brillouin scattering pulse compressor. The maximum energy efficiency of 9% is achieved by employing a circularly-polarized pump pulse at its energy of 50 mJ, around which point the backward stimulated Raman scattering also exhibits a ring-shaped profile. The correlations between spatial and temporal profiles as well as the intensities of the backward stimulated Raman and the stimulated Brillouin scattering generated from Raman cell indicate that the ring-shaped backward stimulated Raman is driven by intense stimulated Brillouin scattering.

Generation of 300 ps laser pulse with 1.2 J energy by stimulated Brillouin scattering in water at 532 nm.

We demonstrate high-energy pulse compression by stimulated Brillouin scattering (SBS) in water using an energy-scalable generator amplifier setup. Pulse compression from 2.4 J, 12 ns at 532 nm to 1.

Optimizing sub-ns pulse compression for high energy application.

We demonstrate ∼ 40X pulse compression (down to ∼ 300 ps) with ∼ 1 joule, nanosecond pulses for high energy applications requiring ≥ 1 gigawatt of peak power. Our method is based on the established principle of stimulated Brillouin scattering (SBS). To push the SBS technique to its highest peak-power limit, a combination of theoretical modeling and experiments is used to identify and optimize all critical parameters, including optical configuration, interaction length, intensity matching, choice of gain medium and thermal stability.

Cryptotanshinone induces inhibition of breast tumor growth by cytotoxic CD4+ T cells through the JAK2/STAT4/ perforin pathway.

Cryptotanshinone (CPT), is a quinoid diterpene isolated from the root of the Asian medicinal plant, Salvia miotiorrhiza bunge. Numerous researchers have found that it could work as a potent antitumor agent to inhibit tumor growth in vitro, buith there has been much less emphasis on its in vivo role against breast tumors. Using a mouse tumor model of MCF7 cells, we showed that CPT strongly inhibited MCF7 cell growth in vivo with polarization of immune reactions toward Th1-type responses, stimulation of naive CD4+ T cell proliferation, and also increased IFN-γ and perforin production of CD4+ T cells in response to tumor-activated splenocytes.

Revision of collagen molecular structure.

Based on the fiber diffraction data from native collagen, Rich and Crick proposed the 10/3-helical model with a 28.6 A axial repeat in 1955 (Rich A.; Crick, F.