Solvent-Free Thermal Synthesis of Extra-Large-Pore Aluminophosphate Zeotype via Self-Assembly of Double-Four-Ring Unit.

The use of Ge as framework atom to direct the formation of double-four-ring (d4r) unit is an effective way to prepare zeolite with extra-large pores, which however also brings about serious problems with regard to production cost and structure stability for their practical application. A new solvent-free thermal synthesis strategy is presented here for facile preparation and molecular-level mechanism study of extra-large-pore aluminophosphate zeotype DNL-1 with -CLO structure. For the first time, the formation of intermediate d4r in the induction period was successfully confirmed, and was correlated with the synthesis condition of low water content and quaternary ammonium template with suitable alkyl chain length.

Gcn5- and Elp3-induced histone H3 acetylation regulates hsp70 gene transcription in yeast.

The purpose of this study was to elucidate the mechanisms by which histone acetylation participates in transcriptional regulation of hsp70 (heat-shock protein 70) genes SSA3 and SSA4 in yeast. Our results indicated that histone acetylation was required for the transcriptional activation of SSA3 and SSA4. The HATs (histone acetyltransferases) Gcn5 (general control non-derepressible 5) and Elp3 (elongation protein 3) modulated hsp70 gene transcription by affecting the acetylation status of histone H3.

hELP3 subunit of the Elongator complex regulates the transcription of HSP70 gene in human cells.

The human Elongator complex is remarkably similar to its yeast counterpart in several aspects. In a previous study, we analyzed the functions of the human elongation protein 3 (hELP3) subunit of the human Elongator by using an in vivo yeast complementation system. However, direct evidence for hELP3 functions in regulating gene expression in human cells was not obtained.