New insights into microbial degradation of polyethylene microplastic and potential polyethylene-degrading bacteria in sediments of the Pearl River Estuary, South China.

Microplastics (MPs) are widely distributed pollutants in various ecosystems, and biodegradation is a crucial process for removal of MPs from environments. Pearl River Estuary, one of the largest estuaries in China, is an important reservoir for MPs with polyethylene MPs (PE-MPs) as the most abundant MPs. Here, biodegradation of PE-MPs and the potential PE-degrading bacteria in sediments of eight major outlets of Pearl River Estuary were firstly investigated.

Specific interaction of resorufin to outer-membrane cytochrome OmcE of Geobacter sulfurreducens: A new insight on artificial electron mediators in promoting extracellular electron transfer.

Bioelectrochemical system (BES) is a unique biotechnology for wastewater treatment and energy recovery, and extracellular electron transfer (EET) between microbe and electrode is the key to optimize the performance of BESs. Resazurin is an effective artificial compound that can promote EET in BESs, but the way how it transports electrons is not fully understood. In this study differential pulse voltammetry revealed that the redox potential of resorufin (RR) (intermediate of resazurin reduction, actual electron mediator) within Geobacter sulfurreducens biofilm was positively shifted by 100 mV than that of free RR, and this shift was attenuated by the mutation of outer-membrane cytochrome gene omcE but not by omcS and omcZ mutation, indicating that RR specifically interacted with OmcE.

Significance of a minor pilin PilV in biofilm cohesion of Geobacter sulfurreducens.

Bacterial adhesion plays a vital role in forming and shaping the structure of electroactive biofilms that are essential for the performance of bioelectrochemical systems (BESs). Type IV pili are known to mediate cell adhesion in many Gram-negative bacteria, but the mechanism of pili-mediated cell adhesion of Geobacter species on anode surface remains unclear. Herein, a minor pilin PilV2 was found to be essential for cell adhesion ability of Geobacter sulfurreducens since the lack of pilV2 gene depressed the cell adhesion capability by 81.

Sub-inhibitory concentrations of ampicillin affect microbial Fe(III) oxide reduction.

Antibiotics are ubiquitous in the iron-rich environments but their roles in microbial reduction of Fe(III) oxides are still unclear. Using ampicillin and Geobacter soli, this study investigated the underlying mechanism by which antibiotic regulated microbial reduction of Fe(III) oxides. Results showed that sub-minimal inhibitory concentrations (sub-MIC) of ampicillin significantly affected ferrihydrite reduction by G.