Surface amphiphilic hybrid porous polymers based on cage-like organosiloxanes for pipette tip solid-phase extraction of microcystins in water.

The occurrence of microcystins (MCs) during harmful algal blooms (HABs) represents a major threat to freshwater environments. In this work, a novel surface amphiphilic hybrid porous polymers based on cage-like organosiloxanes (PCSs) was prepared for the enrichment of MCs. The copolymerization of bifunctional amphiphilic monomers, 2-methacryloyloxyethyl phosphorylcholine (MPC) and N-benzylquininium chloride (BQN), with the cross-linker methacryl substituted polyhedral oligomeric silsesquioxane (POSS) was achieved in an ionic liquid-based porogenic medium.

ERVcancer: a web resource designed for querying activation of human endogenous retroviruses across major cancer types.

Human endogenous retroviruses (HERVs) comprise approximately 8% of the human genome, co-opted into the dynamic regulatory network of cellular potency in early embryonic development. In recent studies, resurgent HERVs' transcriptional activity has been frequently observed in many types of human cancers, suggesting their potential functions in the occurrence and progression of malignancy. However, a dedicated web resource for querying the relationship between the activation of HERVs and cancer development is lacking.

A Comprehensive Review: Versatile Imaging Probe Based on Chemical Materials for Biomedical Applications.

Imaging probe and contrast agents play significant role in combating cancer. Based on special chemical materials, imaging probe can convert cancer symptoms into information-rich images with high sensitivity and signal amplification, accompanying with detection, diagnosis, drug delivery and treatment. In the paper, some inorganic and organic chemical materials as imaging probe, including Ultrasound imaging (US), Optical imaging (OP), Photoacoustic imaging (PA), X-ray Computed Tomography (CT), Magnetic Resonance imaging (MRI), Radionuclide imaging (RNI) probe, as well as multi-modality imaging probe for diagnosis and therapy of tumour were introduced.

Intravenous thrombolysis versus dual antiplatelet therapy in minor ischemic stroke within the thrombolytic window (TAMIS): a multicenter cohort study.

Intravenous thrombolysis (IVT) and dual antiplatelet therapy (DAPT) have been widely used in minor ischemic stroke (MIS) treatment. However, the clinical outcomes and safety of these two treatments have not been compared within the early thrombolytic time window. Here, we conducted a multicenter, ambispective cohort study involving patients with MIS presenting within 4.

Enhancing Electrocatalytic Water Oxidation of NiFe-LDH Nanosheets via Bismuth-Induced Electronic Structure Engineering.

The design and synthesis of high-efficiency electrocatalysts are of great practical significance in electrocatalytic water splitting, specifically in accelerating the slow oxygen evolution reaction (OER). Herein, a self-supported bismuth-doped NiFe layered double hydroxide (LDH) nanosheet array for water splitting was successfully constructed on nickel foam by a one-step hydrothermal strategy. Benefiting from the abundant active sites of two-dimensional nanosheets and electronic effect of Bi-doped NiFe LDH, the optimal BiNiFe LDH electrocatalyst exhibits excellent OER performance in basic media.

Protein O-GlcNAcylation homeostasis regulates facultative heterochromatin to fine-tune sog-Dpp signaling during Drosophila early embryogenesis.

Protein O-GlcNAcylation is a monosaccharide post-translational modification maintained by two evolutionarily conserved enzymes, O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA). Mutations in human OGT have recently been associated with neurodevelopmental disorders, although the mechanisms linking O-GlcNAc homeostasis to neurodevelopment are not understood. Here, we investigate the effects of perturbing protein O-GlcNAcylation using transgenic Drosophila lines that overexpress a highly active OGA.

Ionic liquid assisted in situ growth of nano-confined ionic liquids/metal-organic frameworks nanocomposites for monolithic capillary microextraction of microcystins in environmental waters.

A facile in-situ ionothermal synthesis strategy for fabrication of ionic liquids/metal-organic frameworks (MOFs) (ILs@ZIF-8) nanocomposites hybrid monolith has been proposed to facilitate highly effective capillary microextraction (CME) of ultra-trace microcystins (MCs) in environmental waters. The ZnO nanoparticles (ZnO-NPs) were initially introduced into a precursor polymer monolith, and acted as the metal sources and anchoring seeds to construct ILs@ZIF-8 nanocomposites hybrid monolith via a nanoparticle-directed in-situ growth route in confined imidazolium ionic liquids. Detailed characterization based on scanning electron microscopy (SEM), X-ray diffraction (XRD) and N adsorption-desorption isotherms confirmed that both the morphology and porous structure of ZIF-8 were finely tuned by the incorporation of ILs, which acted as solvents and structure directing agent.

ARID1A loss derepresses a group of human endogenous retrovirus-H loci to modulate BRD4-dependent transcription.

Transposable elements (TEs) through evolutionary exaptation have become an integral part of the human genome, offering ample regulatory sequences and shaping chromatin 3D architecture. While the functional impacts of TE-derived sequences on early embryogenesis have been recognized, their roles in malignancy are only starting to emerge. Here we show that many TEs, especially the pluripotency-related human endogenous retrovirus H (HERVH), are abnormally activated in colorectal cancer (CRC) samples.

photoinitiated fabrication of phosphorylcholine-functionalized polyhedral oligomeric silsesquioxane hybrid monolithic column for mixed-mode capillary electrochromatography.

A monolithic-based mixed-mode stationary phase was prepared for capillary electrochromatography the fast photoinitiated polymerization of 2-methacryloyloxyethyl phosphorylcholine and polyhedral oligomeric silsesquioxane methacrylate (POSS-MA) monomers in the presence of crosslinker pentaerythritol triacrylate (PETA). Several copolymerization parameters, including the composition of monomers or porogens, ratio of crosslinkers to monomers, and polymerization time, were systematically optimized to tune the permeability and efficiency of monolithic columns. The morphologies and structures of the as-prepared monoliths were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, thermogravimetry and nitrogen adsorption/desorption analysis, indicating a typical POSS skeleton morphology with numerous mesopores on the monolith.

Fabrication of a molecularly imprinted monolithic column via the epitope approach for the selective capillary microextraction of neuropeptides in human plasma.

A novel molecularly imprinted monolithic (MIM) column was designed and fabricated using the epitope approach, and was used for the selective capillary microextraction (CME) of the neuropeptides neurotensin (NT) and neuromedin N (NmN). The MIMs were synthesized in a capillary by thermally initiated polymerization of the functional monomer (methacrylic acid (MAA)), in the presence of a dummy template (Pro-Tyr-Ile-Leu (PYIL)), a crosslinker and porogens. The resulting monoliths were characterized by scanning electron microscopy, pore size distribution measurement, and Fourier transform infrared spectroscopy.

Rapid polymerization of polyhedral oligomeric siloxane-based zwitterionic sulfoalkylbetaine monolithic column in ionic liquid for hydrophilic interaction capillary electrochromatography.

A novel polyhedral oligomeric siloxane (POSS)-based zwitterionic monolithic capillary column was prepared via one-pot polymerization in ionic liquid porogen, using N,N-dimethyl-N-methacryloxyethyl-N-(3-sulfopropyl)ammonium betaine (DMMSA) and methacrylic ethyl trimethylammonium chloride (META) as binary functional monomers, and methacryl substituted POSS as cross-linker. The pore structure, permeability and homogeneity were well tuned by optimizing the polymerization conditions. The resultant monolith was characterized by scanning electron microscopy, nitrogen adsorption/desorption isotherm and Fourier transform infrared spectroscopy.

Interphase-arrested Drosophila embryos activate zygotic gene expression and initiate mid-blastula transition events at a low nuclear-cytoplasmic ratio.

Externally deposited eggs begin development with an immense cytoplasm and a single overwhelmed nucleus. Rapid mitotic cycles restore normality as the ratio of nuclei to cytoplasm (N/C) increases. A threshold N/C has been widely proposed to activate zygotic genome transcription and onset of morphogenesis at the mid-blastula transition (MBT).

Preparation of a biomimetic ionic liquids hybrid polyphosphorylcholine monolithic column for the high efficient capillary microextraction of glycopeptide antibiotics.

An ionic liquid hybrid zwitterionic polymer capillary microextraction (CME) column was prepared for the biomimetic enrichment of glycopeptides by one-step copolymerization of 2-methacryloyloxyethyl phosphorylcholine (MPC) and 1-butyl-3-vinylimidazolium bromide, in the presence of crosslinker trimethylolpropane trimethacrylate (TMA). The resultant monolith was characterized by scanning electron microscopy (SEM), fourier transform infrared (FT-IR) spectroscopy and pore size distribution measurement. Due to the incorporation of zwitterionic MPC owning a unique biomimic structure (i.

The relationship between the minor allele content and Alzheimer's disease.

Alzheimer's disease (AD) is a chronic neurodegenerative disease. The genetic risk factors of AD remain better understood. Using previously published dataset of common single nucleotide polymorphisms (SNPs), we studied the association between the minor allele content (MAC) in an individual and AD.

Task-specific solid-phase microextraction based on ionic liquid/polyhedral oligomeric silsesquioxane hybrid coating for sensitive analysis of polycyclic aromatic hydrocarbons by gas chromatography-mass spectrometry.

A direct immersion solid-phase microextraction (DI-SPME) approach for gas chromatography-mass spectrometry (GC-MS) based on hybrid fiber coating of ionic liquid and polyhedral oligomeric silsesquioxane (POSS) is presented. To fabricate the task-specific coating for the enrichment of polycyclic aromatic hydrocarbons (PAHs), 1-butyl-3-vinylimidazolium bis[(trifluoromethyl)sulfonyl]imide (IL) and POSS were rapidly photoinitiated copolymerized within 5 min on a stainless steel fiber. The high efficient extraction of target analytes can be attributed to a combined result of multiple interactions including the strong CF⋯HC pseudohydrogen bonding, π-π stacking, hydrophobic force, and molecular sieve effect.

Ternary thiol-ene photopolymerization for facile preparation of ionic liquid-functionalized hybrid monolithic columns based on polyhedral oligomeric silsesquioxanes.

A simple thiol-ene photopolymerization approach was developed for the rapid preparation of ionic liquid-functionalized hybrid monolithic column based on polyhedral oligomeric silsesquioxane (POSS). "One-pot" polymerization was realized in the UV-transparent fused-silica capillary by using octanethiol, 1-allyl-3-methylimidazolium hexafluorophosphate as functional monomers and methacryl-substituted POSS as a crosslinker. The thiol-vinyl-methacrylate ternary system uniquely exhibits a mixed step-chain growth polymerization regime that combining the thiol-ene reaction and free-radical reaction mechanisms, which provides a simple route to prepare novel POSS-based functionalized hybrid monoliths.

[Determination of peptide antibiotics in animal food by capillary electrochromatography coupled with laser induced fluorescence].

A rapid and sensitive method was established for the analysis of peptide antibiotics (bacitracin, polymyxin B and colistin) in animal food by capillary electrochromatography (CEC) coupled with laser induced fluorescence (LIF) using 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) as fluorogenic reagent. Peptide antibiotics were derivatized by NBD-F in 50 mmol/L borate buffer (pH 7.5) for 45 min at 60℃.

Ultrafast preparation of a polyhedral oligomeric silsesquioxane-based ionic liquid hybrid monolith via photoinitiated polymerization, and its application to capillary electrochromatography of aromatic compounds.

An ionic liquid hybrid monolithic capillary column was prepared within 7 min via photoinitiated free-radical polymerization of an ionic liquid monomer (1-butyl-3-vinylimidazolium-bis[(trifluoromethyl)sulfonyl]imide); VBIMNTF) and a methacryl substituted polyhedral oligomeric silsesquioxane (POSS-MA) acting as a cross-linker. The effects of composition of prepolymerization solution and initiation time on the porous structure and electroosmotic flow (EOF) of monolithic column were investigated. The hybrid monolith was characterized by scanning electron microscopy and FTIR.

On-line coupling of hydrophilic ionic liquids-based polymer monolith microextraction to capillary liquid chromatography with amperometric detection: An ultrasensitive residue analysis method for glycopeptide antibiotics.

A hydrophilic ionic liquids based polymer monolith microextraction (PMME) column (poly(ionic liquid-co-hydroxyethyl methacrylate-co-ethylene dimethacrylate); poly(IL-co-HEMA-co-EDMA)) was prepared for the first time in a capillary and utilized in PMME for the enrichment of glycopeptide antibiotics (GAs), followed by the online coupling analysis of capillary liquid chromatography with amperometric detection (cLC-AD). The prepared monolith exhibited large through pores and good storage stability, as well as a selective extraction machanism for GAs that was attributed to the hydrogen bonding, hydrophilic, electrostatic and π-π interactions between GAs and the imidazolium cations or hydroxyl groups on the surface of absorbent. Several experimental parameters, such as sample flow rate, composition of eluent and solvent desorption conditions, were examined to improve the extraction efficiency of PMME.

Collective effects of common SNPs and risk prediction in lung cancer.

Lung cancer is the leading cause of cancer deaths in both men and women in the US. While most sporadic lung cancer cases are related to environmental factors such as smoking, genetic susceptibility may also play an important role and a number of lung cancer associated single-nucleotide polymorphisms (SNPs) have been identified although many remain to be found. The collective effects of genome-wide minor alleles of common SNPs, or the minor allele content (MAC) in an individual, have been linked with quantitative variations of complex traits and diseases.

Correction: Enrichment of minor allele of SNPs and genetic prediction of type 2 diabetes risk in British population.

[This corrects the article DOI: 10.1371/journal.pone.

Enrichment of minor allele of SNPs and genetic prediction of type 2 diabetes risk in British population.

Type 2 diabetes (T2D) is a complex disorder characterized by high blood sugar, insulin resistance, and relative lack of insulin. The collective effects of genome wide minor alleles of common SNPs, or the minor allele content (MAC) in an individual, have been linked with quantitative variations of complex traits and diseases. Here we studied MAC in T2D using previously published SNP datasets and found higher MAC in cases relative to matched controls.