Bio-orthogonal tuning of matrix properties during 3D cell culture to induce morphological and phenotypic changes.

Described herein is a protocol for producing a synthetic extracellular matrix that can be modified in situ during three-dimensional cell culture. The hydrogel platform is established using modular building blocks employing bio-orthogonal tetrazine (Tz) ligation with slow (norbornene, Nb) and fast (trans-cyclooctene, TCO) dienophiles. A cell-laden gel construct is created via the slow, off-stoichiometric Tz/Nb reaction.

Advancing cell-based therapy in sepsis: An anesthesia outlook.

Sepsis poses a health challenge globally owing to markedly high rates of morbidity and mortality. Despite employing bundle therapy over two decades, approaches including transient organ supportive therapy and clinical trials focusing on signaling pathways have failed in effectively reversing multiple organ failure in patients with sepsis. Prompt and appropriate perioperative management for surgical patients with concurrent sepsis is urgent.

measurement of viscoelastic properties of cellular monolayers graphene strain sensing of elastohydrodynamic phenomena.

Recent advances recognize that the viscoelastic properties of epithelial structures play important roles in biology and disease modeling. However, accessing the viscoelastic properties of multicellular structures in mechanistic or drug-screening applications has challenges in repeatability, accuracy, and practical implementation. Here, we present a microfluidic platform that leverages elastohydrodynamic phenomena, sensed by strain sensors made from graphene decorated with palladium nanoislands, to measure the viscoelasticity of cellular monolayers , without using chemical labels or specialized equipment.

The complex dynamics of the 2023 Kahramanmaraş, Turkey, 7.8-7.7 earthquake doublet.

The destructive 2023 moment magnitude () 7.8-7.7 earthquake doublet ruptured multiple segments of the East Anatolian Fault system in Turkey.

Modeling the Maturation of the Vocal Fold Lamina Propria Using a Bioorthogonally Tunable Hydrogel Platform.

Toward the goal of establishing an engineered model of the vocal fold lamina propria (LP), mesenchymal stem cells (MSCs) are encapsulated in hyaluronic acid (HA)-based hydrogels employing tetrazine ligation with strained alkenes. To mimic matrix stiffening during LP maturation, diffusion-controlled interfacial bioorthogonal crosslinking is carried out on the soft cellular construct using HA modified with a ferocious dienophile, trans-cyclooctene (TCO). Cultures are maintained in MSC growth media for 14 days to afford a model of a newborn LP that is homogeneously soft (nLP), a homogeneously stiffened construct zero (sLP0) or 7 days (sLP7) post cell encapsulation, and a mature LP model (mLP) with a stiff top layer and a soft bottom layer.

Genome-wide identification and transcriptome-based expression profiling of E2 gene family: Implication for potential roles in gonad development of Crassostrea gigas.

In this study, we investigated the role of E2 ubiquitin conjugating enzymes (E2) in the Pacific oyster Crassostrea gigas, with a focus on their involvement in gonad development. We identified 34 E2 genes clustered into nine subgroups and 24 subfamilies. The gene structure and intron-exon location were conserved within the same subfamily, but motif variation suggested functional diversity.

Antibacterial Effect of Shrimp By-Products Hydrolysate on Specific Spoilage Organisms of Squid.

In order to further develop and utilize shrimp processing by-products, in this study, a novel antibacterial hydrolysate of shrimp by-products by pepsin hydrolysis (SPH) was prepared. The antibacterial effect of SPH on specific spoilage organisms of squid after end storage at room temperature (SE-SSOs) was investigated. SPH showed an antibacterial effect on the growth of SE-SSOs, with (23.

Simultaneous Preparation of Chitin and Flavor Protein Hydrolysates from the By-Products of Shrimp Processing by One-Step Fermentation with .

One-step fermentation, inoculated with () in shrimp by-products, was carried out to obtain chitin and flavor protein hydrolysates at the same time. The fermentation conditions were optimized using response surface methodology, resulting in chitin with a demineralization rate of 89.48%, a deproteinization rate of 85.

Rapid character scoring and tabulation of large leaf-image libraries using Adobe Bridge.

Premise: Digital image libraries are an integral part of specimen-based research. However, coding and extracting metadata for hundreds of specimens on a personal computer can be complex. In addition, most existing workflows require downsampling or platform switching and do not link character data directly to the images.

Exploration on the Influencing Factors of Overseas Students' Online Chinese Learning Intention During the Epidemic Period.

In the information age, online teaching has become an essential field of educational development. The work aims to analyze the factors influencing learning intention of Overseas Students (OSs) during the Coronavirus Disease 2019 (COVID-19). The work adopts the method of Unified Theory of Acceptance and Use of Technology (UTAUT), and implements the influencing factor model of open source software online Chinese learning (OCL).

First fossil-leaf floras from Brunei Darussalam show dipterocarp dominance in Borneo by the Pliocene.

The Malay Archipelago is one of the most biodiverse regions on Earth, but it suffers high extinction risks due to severe anthropogenic pressures. Paleobotanical knowledge provides baselines for the conservation of living analogs and improved understanding of vegetation, biogeography, and paleoenvironments through time. The Malesian bioregion is well studied palynologically, but there have been very few investigations of Cenozoic paleobotany (plant macrofossils) in a century or more.

An image dataset of cleared, x-rayed, and fossil leaves vetted to plant family for human and machine learning.

Leaves are the most abundant and visible plant organ, both in the modern world and the fossil record. Identifying foliage to the correct plant family based on leaf architecture is a fundamental botanical skill that is also critical for isolated fossil leaves, which often, especially in the Cenozoic, represent extinct genera and species from extant families. Resources focused on leaf identification are remarkably scarce; however, the situation has improved due to the recent proliferation of digitized herbarium material, live-plant identification applications, and online collections of cleared and fossil leaf images.

Improved fatty acid profile reduces body fat and arterial stiffness in obese adolescents upon combinatorial intervention with exercise and dietary restriction.

Objective: In order to examine the effect of 4-week combinatorial intervention with exercise training and dietary restriction on serum fatty acids, and to explore the correlation of intervention-induced improvement of serum fatty acid profile with the reduction of body fat and arterial stiffness.

Methods: Thirty-three obese adolescents were randomized into the intervention (n = 19) and control (n = 14) groups. The participants from the intervention group were subjected to 4-week combinatorial intervention with exercise training and dietary restriction while the participants from the control group maintained regular activities and diet habits.

Glutaredoxin1 knockout promotes high-fat diet-induced obesity in male mice but not in female ones.

This study aims to explore how a high-fat diet and glutaredoxin1 (Glrx1) deficiency affect the development of obesity in male and female mice. A high-fat diet induced great differences in calorie intake and body weight gain between male and female mice; furthermore, the Glrx1 deficiency made male mice more sensitive to a high-fat diet than females. Male mice had higher glucose intolerance, and Glrx1 deficiency aggravated gender differences in glucose intolerance.

Structural Changes and Evolution of Peptides During Chill Storage of Pork.

In this work, we investigated changes in protein structures in vacuum-packed pork during chill storage and its impact on the protein digestion. muscles were vacuum packed and stored at 4°C for 3 days. Samples were subjected to Raman spectroscopy, digestion and nano LC-MS/MS.

Influence of salting process on the structure and in vitro digestibility of actomyosin.

Salting process is widely used in the process of meat products, whereas few studies have revealed the digestibility of actomyosin after salting treatment, which is closely related with the nutrition of meat. This work reported effect of salting on the structural change and digestibility of actomyosin before and after heat treatment. Actomyosin in 0.

Pork Meat Proteins Alter Gut Microbiota and Lipid Metabolism Genes in the Colon of Adaptive Immune-Deficient Mice.

Scope: Excessive consumption of processed meat has been linked to an increasing risk of gut diseases. It is investigated how pork meat proteins affect colon homeostasis between normal and immune-compromised mice.

Methods And Results: Immune-deficient mice (Rag1 ) and wild-type mice are fed a diet that contains 20% casein or protein isolated from cooked pork or dry-cured pork for 3 months.

Enhanced electrochemical phosphate recovery from livestock wastewater by adjusting pH with plant ash.

In the field of environmental wastewater treatment, it is a very meaningful topic to recover phosphate from swine wastewater in the form of struvite precipitation. The solution pH is one of the important influencing factors in the process of struvite precipitation. In this paper, an attempt was made to recover the phosphate from swine wastewater by adding plant ash.

Influence of hydrothermal treatment on the structural and digestive changes of actomyosin.

Background: Heat treatment induces both structural and digestive change of meat protein. However, little has been revealed regarding the associations between structural changes and digested peptides of myofibrillar proteins. This work investigated the effects of heat treatment on the structures and in vitro digestibility of actomyosin, and the peptidomics of the digests were analyzed using liquid chromatography tandem mass spectrometry (LC-MS/MS).

Protective effects of the flavonoid fraction obtained from pomelo fruitlets through ultrasonic-associated microwave extraction against AAPH-induced erythrocyte hemolysis.

Pomelo fruitlet is a side-product of pomelo, and this study aimed to extract the antioxidative flavonoid compounds from pomelo fruitlets with high efficiency through ultrasonic-associated microwave methods. Scanning electron microscopy analysis indicated that the spatial structure of the pomelo fruitlet powder was changed; microwaves and ultrasonic waves facilitated the formation of globular and curved surfaces, respectively. Ultrasonic-microwave synergistic pretreatment resulted in significantly higher yield.

In vitro protein digestion of pork cuts differ with muscle type.

Porcine trapezius, longissimus dorsi and biceps femoris muscles have been shown to contain different types of muscle fibers. Little is known about the differences in digestibility among cooked pork cuts. In this study, we compared the protein losses of porcine trapezius, longissimus dorsi and biceps femoris muscles under in vitro digestion conditions.

In vitro protein digestibility of pork products is affected by the method of processing.

Processing contributes to different flavors and textures of pork products. However, processing methods have also showed a great impact on meat nutrition. In this study, protein digestibility and digested products were compared among four kinds of processed pork products (cooked pork, emulsion-type sausage, dry-cured pork and stewed pork).

Single-base resolution maps of cultivated and wild rice methylomes and regulatory roles of DNA methylation in plant gene expression.

Background: DNA methylation plays important biological roles in plants and animals. To examine the rice genomic methylation landscape and assess its functional significance, we generated single-base resolution DNA methylome maps for Asian cultivated rice Oryza sativa ssp. japonica, indica and their wild relatives, Oryza rufipogon and Oryza nivara.