Study on Preparation Process of Anticoagulant BAY2433334.

A new process route suitable for the industrial production of BAY2433334 has been developed in this paper, which avoids the patent limitations of the originator company of BAY2433334 to the preparation of BAY2433334. BAY2433334 is obtained from (2)-2-aminobutyric acid by esterification, diazotization, condensation reactions, deacetyl deprotection, activation reactions, and Mitsunobu reactions. This method is simple to operate, and the raw materials are inexpensive and readily available.

Recent Advances in Cellulose Nanofiber Modification and Characterization and Cellulose Nanofiber-Based Films for Eco-Friendly Active Food Packaging.

There is growing interest in the use of bio-based materials as viable alternatives to petrochemical-based packaging. However, the practical application of bio-based films is often hampered by their poor barrier and poor mechanical properties. In this context, cellulose nanofibers (CNFs) have attracted considerable attention owing to their exceptional biodegradability, high aspect ratio, and large surface area.

Habitat-based MRI radiomics to predict the origin of brain metastasis.

Background: This study aims to explore the value of habitat-based magnetic resonance imaging (MRI) radiomics for predicting the origin of brain metastasis (BM).

Purpose: To investigate whether habitat-based radiomics can identify the metastatic tumor type of BM and whether an imaging-based model that integrates the volume of peritumoral edema (VPE) can enhance predictive performance.

Methods: A primary cohort was developed with 384 patients from two centers, which comprises 734 BM lesions.

HPClas: A data-driven approach for identifying halophilic proteins based on catBoost.

Halophilic proteins possess unique structural properties and show high stability under extreme conditions. This distinct characteristic makes them invaluable for application in various aspects such as bioenergy, pharmaceuticals, environmental clean-up, and energy production. Generally, halophilic proteins are discovered and characterized through labor-intensive and time-consuming wet lab experiments.

Biowaste-Derived Carbon Dots-Based Molecularly Imprinted Fluorescent Nanosensor for Selective Detection of Rutin.

In this work, Waste pine nut shells were used as organic carbon source of biomass to synthesize carbon quantum dots. A highly responsive and selective fluorescent nanosensor (Si-doped biomass-derived carbon dots with molecular imprinted polymers, Si-CDs@MIPs) was designed for determination of Rutin (RT) in Chinese herbal substances like Sophora japonica L..

The conserved protein DopA is required for growth, drug tolerance and virulence in Aspergillus fumigatus.

The majority of Aspergillus fumigatus reproduction occurs asexually, with large numbers of conidiophores producing small hydrophobic conidia dispersed aerially. When healthy hosts inhale conidia, the mucosal cilia and phagocytosis by the innate immune system can remove them. However, in immunocompromised hosts, the conidia are not removed, which allows them to germinate, forming mycelium that invades host tissues and causes disease.

Microenvironmental modulation breaks intrinsic pH limitations of nanozymes to boost their activities.

Functional nanomaterials with enzyme-mimicking activities, termed as nanozymes, have found wide applications in various fields. However, the deviation between the working and optimal pHs of nanozymes has been limiting their practical applications. Here we develop a strategy to modulate the microenvironmental pHs of metal-organic framework (MOF) nanozymes by confining polyacids or polybases (serving as Brønsted acids or bases).

SCREEN: A Graph-based Contrastive Learning Tool to Infer Catalytic Residues and Assess Enzyme Mutations.

Unlabelled: The accurate identification of catalytic residues contributes to our understanding of enzyme functions in biological processes and pathways. The increasing number of protein sequences necessitates computational tools for the automated prediction of catalytic residues in enzymes. Here, we introduce SCREEN, a graph neural network for the high-throughput prediction of catalytic residues via the integration of enzyme functional and structural information.

Mindfulness acting with awareness and emotional eating among polycystic ovary syndrome women with infertility: the mediating role of depression.

Emotional eating, characterized by the tendency to increase food intake in response to negative emotional states, is often linked to poor emotion regulation. While mindfulness-based interventions have been studied for their benefits in reducing emotional eating, less is known about how inherent mindfulness traits, relate to emotional regulation particularly among individuals with polycystic ovary syndrome (PCOS), a population known for high rates of psychological distress and disordered eating behaviors. This study investigates the associations between different facets of mindfulness, depressive symptoms, and emotional eating among individuals with PCOS and infertility who had not received any formal mindfulness intervention.

Photothermal Miniemulsion Polymerization by Amphiphilic Gold Nanoclusters.

Gold nanoclusters (AuNCs), which are approximately 2 nm in size, exhibit distinctive photophysical and catalytic properties, but their performance is often compromised by environmental factors. To mitigate these challenges, attempts have been made to incorporate AuNCs into polymer matrices to enhance their stability. Miniemulsion polymerization has proven to be an effective method for fabricating organic-inorganic composites.

Tumour necrosis factor-like weak inducer of apoptosis participates in the development of cutaneous fibrosis in an experimental systemic sclerosis model.

Objectives: Systemic sclerosis (SSc), a chronic autoimmune disorder, characterised by local inflammation and progressive fibrosis. Tumour necrosis factor-like weak inducer of apoptosis (TWEAK) has been established as a key mediator in fibrotic processes across multiple organs, primarily through binding to its receptor, fibroblast growth factor-inducible 14 (Fn14). However, the precise role of the TWEAK/Fn14 signalling in SSc pathogenesis remains unclear.

Caveolin-1 regulates context-dependent signaling and survival in Ewing Sarcoma.

Cellular plasticity is a hallmark function of cancer, but many of the underlying mechanisms are not well understood. In this study, we identify Caveolin-1, a scaffolding protein that organizes plasma membrane domains, as a context-dependent regulator of survival signaling in Ewing sarcoma (EwS). Single cell analyses reveal a distinct subpopulation of EwS cells, which highly express the surface marker CD99 as well as Caveolin-1.

Resting state EEG delta-beta amplitude-amplitude coupling: a neural predictor of cortisol response under stress.

Unlabelled: Stress is ubiquitous in daily life. Subcortical and cortical regions closely interact to respond to stress. Delta-beta cross-frequency coupling (CFC), believed to signify communication between different brain areas, can serve as a neural signature underlying the heterogeneity in stress responses.

Dynamic effects of fixed orthodontic treatment on oral health and oral microbiota: a prospective study.

Objective: This prospective clinical study examined the effects of fixed orthodontic appliances on oral hygiene and assessed changes in the oral microflora structure of orthodontic patients using high-throughput sequencing technology.

Methods: A total of 45 patients undergoing initial fixed orthodontic treatment were recruited from three hospitals in Beijing, China. Clinical parameters and oral hygiene habits questionnaire were recorded at pre-treatment (T0) and at a 6-month follow-up (T1).

Effect of traction therapy on muscle satellite cell proliferation and differentiation in a rat model of knee stiffness.

Background: In the rat knee stiffness model, the duration of traction treatment is mostly 20-40 min; however, relatively few studies have been conducted on longer traction treatment of extended knee stiffness in rats. Therefore, the aim of this study was to explore the efficacy of prolonged traction and its mechanism of action in extended knee stiffness in rats.

Methods: The model of extended knee joint stiffness was established in rats and treated with powered flexion position traction.

A Montmorillonite-Based Pickering Nanoemulsion for the Integration of Photothermal Therapy and NIR-Responsive Drug Delivery.

Chemo/photothermal combination therapy is a promising and practical approach for cancer treatment which calls for certain nanovehicles to achieve the spatiotemporal co-occurrence of photothermal conversion and drug delivery. Herein, we developed a montmorillonite-based Pickering emulsion equipped with a near-infrared photothermal agent (indocyanine green, ICG) and anticarcinogen (paclitaxel, PTX). With both montmorillonite and ICG functioning as interfacial stabilizers, the Pickering emulsion showed good stability and nanoscale droplet size, which were favored for cellular applications.

Copper-based biomimetic nanozymes with multi-enzyme activity for phosphate detection.

Nanozymes are nanoparticles with enzymatic activity, which are widely used in environmental and antibacterial research. Herein, we designed and synthesized novel amorphous nanozyme Cu-Im NPs with multiple enzyme-mimicking activities. Cu-Im NPs have the same active sites as natural laccase.

MORE: a multi-omics data-driven hypergraph integration network for biomedical data classification and biomarker identification.

High-throughput sequencing methods have brought about a huge change in omics-based biomedical study. Integrating various omics data is possibly useful for identifying some correlations across data modalities, thus improving our understanding of the underlying biological mechanisms and complexity. Nevertheless, most existing graph-based feature extraction methods overlook the complementary information and correlations across modalities.

Wheat WW Domain-Containing Protein TaCFL1 Negatively Regulates Cuticular Wax Biosynthesis.

Waxy cuticle covers plant aerial organs and protects plants against environmental challenges. Although improved cuticle-associated traits are aimed at the wheat breeding programs, the mechanism governing wheat cuticular wax biosynthesis remains to be elucidated. Herein, wheat WW domain-containing protein TaCFL1 is characterized as a negative regulator of wax biosynthesis.

The microRNA-29ab1/Zfp36/AR Axis in the Hypothalamus Regulates Male-Typical Behaviors in Mice.

Male-typical behaviors such as aggression and mating, which reflect sexual libido in male mice, are regulated by the hypothalamus, a crucial part of the nervous system. Previous studies have demonstrated that microRNAs (miRNAs), especially , play a vital role in reproduction and the neural control of behaviors. However, it remains unclear whether affects reproduction through the hypothalamus-mediated regulation of male-typical behaviors.

Identification and expression analysis of lncRNAs in rice roots (Oryza sativa L.) under elevated CO concentration and/or cadmium stress.

The gradual rise of CO is one of the global climate changes, Cd stress is also a major abiotic stress factor that affects rice (Oryza sativa L.). The rice seedlings were treated under two CO concentrations and two CdCl concentrations for 7 days (treatments names: 400 ± 20 μmol mol CO and 0 μmol L CdCl concentrations, AC; 400 ± 20 μmol mol CO and 150 μmol L CdCl concentrations, Cd; 800 ± 20 μmol mol CO and 0 μmol L CdCl concentrations, EC; 800 ± 20 μmol mol CO and 150 μmol L CdCl concentrations, EC + Cd).

Coumarin-based fluorescent probes for the detection of copper (II) and imaging in mice of Wilson's disease.

Copper is an essential redox-active metal nutrient, with tightly regulated homeostasis. Copper homeostasis is closely linked to various diseases, making it a critical area of study. Fluorescent probes serve as powerful tools for investigating these conditions.