Crystal-Facet-Controlled Internal Electric Field in MOF/COF Heterojunction Towards Efficient Photocatalytic Overall Water Splitting.

Covalently integrating two type of crystalline porous materials, metal-organic frameworks (MOFs) and covalent organic frameworks (COFs), to form heterostructure photocatalysts inheriting their structural merits has shown inherent advantages in hydrogen evolution reaction. However, how to control the internal electric field in aimed MOF/COF heterojunction to achieve an improved photocatalytic activity is still ambiguous. Herein, for the first time, we report a rational control of the internal electric field in MOF/COF heterojunction by engineering the crystal facet of MOFs to achieve enhanced photocatalytic overall water splitting (OWS) activity.

Data-knowledge co-driven feature based prediction model via photoplethysmography for evaluating blood pressure.

Background: Knowledge feature (KF) with clear physiological significance of photoplethysmography are widely used in predicting blood pressure. However, KF primarily focus on local information of photoplethysmography, which may struggle to capture the overall characteristics.

Methods: Firstly, functional data analysis (FDA) was introduced to extract two types of data feature (DF).

Synthesis of iron phthalocyanine/CeO Z-scheme nanocomposites as efficient photocatalysts for degradation of 2,4-dichlorophenol.

Modulating the photogenerated electrons of CeO to activate O and efficiently photocatalytic degradation of chlorophenols is a highly desired goal. Herein, we have successfully fabricated an FePc/CeO heterojunction through H-bond induced assembly. The photocatalytic degradation of 2,4-DCP by the amount-optimized FePc/CeO nanocomposite was improved by 3 times compared with that by pure CeO.

LEUTX regulates porcine embryonic genome activation in somatic cell nuclear transfer embryos.

Emerging evidence highlights the regulatory role of paired-like (PRD-like) homeobox transcription factors (TFs) in embryonic genome activation (EGA). However, the majority of PRD-like genes are lost in rodents, thus prompting an investigation into PRD-like TFs in other mammals. Here, we showed that PRD-like TFs were transiently expressed during EGA in human, monkey, and porcine fertilized embryos, yet they exhibited inadequate expression in their cloned embryos.

The Effectiveness of Four Nicotinamide Adenine Dinucleotide (NAD) Precursors in Alleviating the High-Glucose-Induced Damage to Hepatocytes in : Evidence in NAD Homeostasis, Sirt1/3 Activation, Redox Defense, Inflammatory Response, Apoptosis, and Glucose Metabolism.

The administration of NAD precursors is a potential approach to protect against liver damage and metabolic dysfunction. However, the effectiveness of different NAD precursors in alleviating metabolic disorders is still poorly elucidated. The current study was performed to compare the effectiveness of four different NAD precursors, including nicotinic acid (NA), niacinamide (NAM), nicotinamide riboside (NR), and nicotinamide mononucleotide (NMN) in alleviating high-glucose-induced injury to hepatocytes in a fish model, .

Development and application of a competitive ELISA for the detection of antibodies against Abortusequi in equids.

The high abortion rate associated with Abortusequi (. Abortusequi) infection in equids has re-emerged over the past 10 years and has caused serious economic losses to China. Our previous studies showed that the flagellin FljB gene could distinguish .

KLF4 facilitates chromatin accessibility remodeling in porcine early embryos.

Chromatin accessibility remodeling driven by pioneer factors is critical for the development of early embryos. Current studies have illustrated several pioneer factors as being important for agricultural animals, but what are the pioneer factors and how the pioneer factors remodel the chromatin accessibility in porcine early embryos is not clear. By employing low-input DNase-seq (liDNase-seq), we profiled the landscapes of chromatin accessibility in porcine early embryos and uncovered a unique chromatin accessibility reprogramming pattern during porcine preimplantation development.

Mechanism of an RBM-targeted rabbit monoclonal antibody 9H1 neutralizing SARS-CoV-2.

The COVID-19 pandemic, caused by SARS-CoV-2, has led to over 750 million infections and 6.8 million deaths worldwide since late 2019. Due to the continuous evolution of SARS-CoV-2, many significant variants have emerged, creating ongoing challenges to the prevention and treatment of the pandemic.

Development and evaluation of a blocking ELISA for serological diagnosis of equine infectious anemia.

Equine infectious anemia (EIA) is an important viral disease characterized by persistent infection in equids worldwide. Most EIA cases are life-long virus carriers with low antibody reactions and without the appearance of clinical symptoms. A serological test with high sensitivity and specificity is required to detect inapparent infection.

Development and Application of Real-Time PCR Assay for Detection of Salmonella Abortusequi.

Salmonella enterica subsp. serovar Abortusequi is a major pathogen in horse and donkey herds, causing abortion in pregnant equids and resulting in enormous economic losses. A rapid and reliable method is urgently needed to detect .

Engineering β-ketoamine covalent organic frameworks for photocatalytic overall water splitting.

Covalent organic frameworks (COFs) are an emerging type of crystalline and porous photocatalysts for hydrogen evolution, however, the overall water splitting activity of COFs is rarely known. In this work, we firstly realized overall water splitting activity of β-ketoamine COFs by systematically engineering N-sites, architecture, and morphology. By in situ incorporating sub-nanometer platinum (Pt) nanoparticles co-catalyst into the pores of COFs nanosheets, both Pt@TpBpy-NS and Pt@TpBpy-2-NS show visible-light-driven overall water splitting activity, with the optimal H and O evolution activities of 9.

Clinical Effect of Feldspar Glass Ceramics and Lithium Disilicate Reinforced Glass Ceramics.

The clinical effect of feldspar ceramics and lithium disilicate reinforced glass ceramics was studied. 57 affected teeth with complete root canal treatment and computer aided design/computer aided manufacturing (CAD/CAM) endocrown restoration were reviewed 24-36 months aftercompletion of the restoration. These dimensions including anatomical morphology, proximal contact , color matching, edge integrity, edge coloring, secondary caries, kinesthetic occlusion relationship, and periodontal health of the affected teeth by the modified clinical scoringcriteriafor United States Public Health Service (USPHS) were evaluated, and the patients' satisfaction was recorded.

Ultrathin Phosphate-Modulated Co Phthalocyanine/g-CN Heterojunction Photocatalysts with Single Co-N (II) Sites for Efficient O Activation.

Realization of solar-driven aerobic organic transformation under atmospheric pressure raises the great challenge for efficiently activating O by tailored photocatalysts. Guided by theoretical calculation, phosphate groups are used to induce the construction of ultrathin Co phthalocyanine/g-CN heterojunctions (CoPc/P-CN, ≈4 nm) via strengthened H-bonding interfacial connection, achieving an unprecedented 14-time photoactivity improvement for UV-vis aerobic 2,4-dichlorophenol degradation compared to bulk CN by promoted activation of O. It is validated that more O radicals are produced through the improved photoreduction of O by accelerated photoelectron transfer from CN to the ligand of CoPc and then to the abundant single Co-N (II) catalytic sites, as endowed by the matched dimension, intimate interface even at the molecular level, and high CoPc dispersion of resulted heterojunctions.

The synthesis of interface-modulated ultrathin Ni(ii) MOF/g-CN heterojunctions as efficient photocatalysts for CO reduction.

It is highly desirable to improve charge separation and to provide catalytic functions for the efficient photocatalytic CO2 reduction reaction (CO2RR) on g-C3N4 (CN). Here, dimension-matched ultrathin NiMOF/CN heterojunctions have been successfully constructed by the in situ growth of NiMOF nanosheets on hydroxylated and 1,4-aminobenzoic acid (AA) functionalized CN nanosheets, respectively, with ultrasonic assistance. The resultant NiMOF/CN heterojunctions exhibited excellent photocatalytic activities for the CO2RR to produce CO and CH4, especially NiMOF/CN-AA, which had photoactivity 18 times higher than that of bare CN.

Mg-O-Bridged Polypyrrole/g-C N Nanocomposites as Efficient Visible-Light Catalysts for Hydrogen Evolution.

It is highly desired to improve the visible-light activity of g-C N for H evolution by constructing closely contacted heterojunctions with conductive polymers. Herein, a polymer nanocomposite photocatalyst with high visible-light activity is fabricated successfully by coupling nanosized polypyrrole (NPPy) particles onto g-C N nanosheets through a simple wet-chemical process, and its visible-light activity is improved further by constructing Mg-O bridges between the NPPy and g-C N . The amount-optimized bridged nanocomposite displays an approximately ninefold improvement in visible-light activity compared with g-C N .

Equine Mx1 Restricts Influenza A Virus Replication by Targeting at Distinct Site of its Nucleoprotein.

Interferon-mediated host factors myxovirus (Mx) proteins are key features in regulating influenza A virus (IAV) infections. Viral polymerases are essential for viral replication. The Mx1 protein has been known to interact with viral nucleoprotein (NP) and PB2, resulting in the influence of polymerase activity and providing interspecies restriction.

Identification and characterization of sequence signatures in the Bacillus subtilis promoter P for tuning promoter strength.

Objective: To thoroughly characterize the P promoter and identify the elements that affect the promoter activity.

Result: The sequences flanking the - 35 and - 10 box of the P promoter were divided into six segments, and six random-scanning mutant promoter libraries fused to an enhanced green fluorescent protein EGFP were made and analyzed by flow cytometry. Our results showed that the four nucleotides flanking the - 35 box could mostly influence the promoter activity, and this influence was related to the GC content.

Development of an EvaGreen-based real-time PCR assay for detection of Aleutian mink disease virus.

The objective of this study was to develop a rapid, sensitive and specific EvaGreen (EG)-based real-time PCR assay capable of detecting Aleutian mink disease virus (AMDV) and to evaluate the reliability of the assay for analysis of blood or tissue samples. For this assay, a pair of primers was designed based on a nonstructural protein (NS)-encoding gene of AMDV, and the identity of PCR products was identified based on a melting temperature of 82.8°C.

Improving Cadmium Resistance in Through Continuous Genome Evolution.

Cadmium (Cd) is a heavy metal that is extremely toxic to many organisms; however, microbes are highly adaptable to extreme conditions, including heavy metal contamination. Bacteria can evolve in the natural environment, generating resistant strains that can be studied to understand heavy-metal resistance mechanisms, but obtaining such adaptive strains usually takes a long time. In this study, the genome replication engineering assisted continuous evolution (GREACE) method was used to accelerate the evolutionary rate of the genome to screen for mutants with high resistance to cadmium.

Presyncodon, a Web Server for Gene Design with the Evolutionary Information of the Expression Hosts.

In the natural host, most of the synonymous codons of a gene have been evolutionarily selected and related to protein expression and function. However, for the design of a new gene, most of the existing codon optimization tools select the high-frequency-usage codons and neglect the contribution of the low-frequency-usage codons (rare codons) to the expression of the target gene in the host. In this study, we developed the method Presyncodon, available in a web version, to predict the gene code from a protein sequence, using built-in evolutionary information on a specific expression host.