Transcriptomic analysis of Zhang in transition to the viable but non-culturable state by RNA sequencing.

Background: Some bacteria enter the viable but non-culturable (VBNC) state to survive harsh environmental conditions and external stresses. This alters cell physiology and has implications for the food industry as some bacteria, such as lactobacilli, undergo similar changes during food processing.

Methods: This study aimed to investigate the transcriptomic changes of a probiotic strain, Zhang ( Zhang), upon transition to the VBNC state using high throughput RNA sequencing (RNA-seq).

Metabolomic Differences between Viable but Nonculturable and Recovered Zhang.

The fermentation process can be affected when the starter culture enters the viable but nonculturable (VBNC) state. Therefore, it is of interest to investigate how VBNC cells change physiologically. (.

Comparative Analysis Using Raman Spectroscopy of the Cellular Constituents of Zhang in a Normal and Viable but Nonculturable State.

This study aimed to investigate the molecular composition of a viable but nonculturable (VBNC) state of a probiotic strain, Zhang ( Zhang), using single-cell Raman spectroscopy (SCRS). Fluorescent microcopy with live/dead cell staining (propidium iodide and SYTO 9), plate counting, and scanning electron microscopy were used in combination to observe bacteria in an induced VBNC state. We induced the VBNC state by incubating the cells in de Man, Rogosa, and Sharpe broth (MRS) at 4 °C.