Influence of Mothers/Grandmothers Coviewing Cartoons With Children on Children's Viewing Experience.

Watching cartoons is one important event in children's early lives. This activity is highly influential on many factors, such as children's cognitive and behavioral development. Some researchers believe that parents should coview cartoons with children to help them filter and distinguish useful content.

Methylation of the DKK3 promoter is associated with poor prognosis in patients with cervical adenocarcinoma.

Objective: The aim of this study was to investigate the mRNA of DKK3 (Dickkopf-3) in cervical adenocarcinoma, and to explore correlations between methylation status of the DKK3 promoter and biological behaviors of cervical adenocarcinoma.

Methods: The mRNA expression level of DKK3 was detected by real-time quantitative reverse transcription PCR. Methylation-specific PCR (MSP) analysis was performed to detect the methylated degrees of the DNA of the DKK3 promoter.