Characteristics of a Colistin-Resistant ST695 Harboring the Chromosomally-Encoded m Gene.

having chromosomally-encoded is rarely reported. In this study, we recovered a chromosomal carrying , designated HeN100, from the feces of a diarrheal pig in China. Antimicrobial susceptibility testing showed that HeN100 was resistant to three aminoglycosides, twelve β-lactams including three carbapenems, one phenicol, two tetracyclines, two fluoroquinolones, nitrofurantoin, and colistin tested.

Involvement of P2X7 receptors in satellite glial cells of dorsal root ganglia in the BmK I -induced pain model of rats.

The P2X7 receptor (P2X7R) plays an important role in inflammatory and neuropathic pain. Our recent study indicated that activation of P2X7R in microglial cells of spinal cord contributes to the inflammatory pain induced by BmK I, the major active compound from Buthus martensi Karsch (BmK). In the present study, we further investigated whether P2X7R in satellite glial cells (SGCs) of dorsal root ganglion (DRG) is involved in the BmK I-induced pain in rats.

Molecular Characterization and Expression of , and and Their Association with Growth Traits in Sheep.

The , , and are located on chromosome 6 of sheep and a domain of 36.15-38.56 Mb, which plays an essential role in tissue and embryonic growth.

Loading paclitaxel into porous starch in the form of nanoparticles to improve its dissolution and bioavailability.

In this work, paclitaxel was loaded into porous starch in the form of nanoparticles (PNPS), and the properties of PNPS were investigated by using raw paclitaxel and the system of paclitaxel directly loaded into porous starch (PPS) as control groups. According to the tested results, the drug loading (DL) and encapsulation efficiency (EE) of PNPS were 14.13%±0.

Optimal cut-off threshold in pulse pressure predicting cardiovascular death among newly diagnosed end-stage renal disease patients: A prospective cohort study.

Cardiovascular disease (CVD) is the leading cause of death in patients with end-stage renal disease (ESRD) treated by dialysis. Pulse pressure (PP) as an independent prognostic factor of cardiovascular risk might be clinically implicated in predicting the short-term deaths due to cardiovascular diseases in ESRD patients. This study aimed to investigate the dose-response association between PP and risk of cardiovascular mortality in patients initializing peritoneal dialysis (PD).

[The risk factors and preventive measures of hospital infections on preoperative children with cleft lips and palates].

Objective: To analyze the risk factors of preoperative upper respiratory infections in children with cleft lips and palate (CLP) and investigate preventive measures to reduce infections and improve the quality of treatments.

Methods: A total of 510 children with CLP of ages 3 years old or younger were selected from hospital cases from June to December 2017. The test group comprised 50 children with upper respiratory infections, whereas the control group comprised 460 children without upper respiratory infections.

WO nanosheets rich in oxygen vacancies for enhanced electrocatalytic N reduction to NH.

The Haber-Bosch process for industrial-scale NH3 production suffers from harsh conditions and serious CO2 release. Electrochemical N2 reduction is an alternative approach to synthesize NH3 under ambient conditions, but it requires highly-efficient electrocatalysts for the N2 reduction reaction (NRR). In this Communication, we demonstrate that WO3 nanosheets rich in oxygen vacancies (R-WO3 NSs) exhibit greatly enhanced NRR performances.

[Investigation on knowledge, attitude and practice (KAP) of foodborne parasitic diseases among medical students].

Objective: To understand the knowledge, attitude and practice (KAP) of foodborne parasitic diseases among medical college students, so as to provide the evidence for the development of strategies on health education.

Methods: A total of 929 premed students from a medical college were selected in Hebei Province using the stratified sampling method from October to December, 2017, and investigated by questionnaires to understand their KAP on foodborne parasitic diseases.

Results: The understanding and concern degree on foodborne parasitic diseases among the college students from different majors were statistically different (all < 0.

A fluorescein-based AND-logic FPSi probe for the simultaneous detection of Hg and F.

In this study, we have designed and synthesized a novel 'AND' logic based fluorescence probe,1-(3',6'-bis((tert-butyldiphenylsilyl)oxy)-3-oxospiro (isoindoline-1,9'-xanthen)-2-yl)-3-phenylthiourea (FPSi), for the rapid (3 min) simultaneous detection of F and Hg in DMSO/HO solution (7:3, v/v). The FPSi probe, synthesized over three steps starting from commercially available fluorescein, was constructed by attaching tert-butyldiphenylsilyl and thiosemicarbazide (as the specific identification groups for F and Hg) to the skeleton of fluorescein, respectively. FPSi produced no fluorescence response towards the addition of F or Hg separately.

Deep Genome Resequencing Reveals Artificial and Natural Selection for Visual Deterioration, Plateau Adaptability and High Prolificacy in Chinese Domestic Sheep.

Sheep were one of the earliest domesticated animals. Both artificial and natural selection during domestication has resulted in remarkable changes in behavioral, physiological, and morphological phenotypes; however, the genetic mechanisms underpinning these changes remain unclear, particularly for indigenous Chinese sheep. In the present study, we performed pooled whole-genome resequencing of 338 sheep from five breeds representative of indigenous Chinese breeds and compared them to the wild ancestors of domestic sheep (Asian mouflon, ) for detection of genome-wide selective sweeps.

Relationship between psychosocial adaptation and health-related quality of life of patients with stoma: A descriptive, cross-sectional study.

Objective: To investigate the relationship between psychosocial adaptation and quality of life of patients with ostomies and to provide reference data for clinical care practice.

Background: Surgical enterostomy impacts the life of patient. Most patients are transferred to home or community hospital after discharge and require long-term nursing care.

Scutellarin exerts protective effects against atherosclerosis in rats by regulating the Hippo-FOXO3A and PI3K/AKT signaling pathways.

Atherosclerosis (AS), a progressive disorder, is one of the tough challenges in the clinic. Scutellarin, an extract from Herba Erigerontis, is found to have oxygen-free radicals scavenging effects and antioxidant effects. In this study, we aimed to investigate the anti-AS effects of scutellarin is related to controlling the Hippo-FOXO3A and PI3K/AKT signal pathway.

Rosmarinic acid exerts an antagonistic effect on vascular calcification by regulating the Nrf2 signalling pathway.

Vascular calcification (VC) is a process in which calcium phosphate crystals deposit within the intima and middle membrane of the vascular wall. Rosmarinic acid (RA) is a common phenolic compound. It possesses antioxidation, anti-inflammatory, antimicrobial effects.

Up-regulation of P2X7 Receptors Contributes to Spinal Microglial Activation and the Development of Pain Induced by BmK-I.

Previous work has demonstrated that the sensitization of spinal neurons and microglia is important in the development of pain behaviors induced by BmK I, a Na channel activator and a major peptide component of the venom of the scorpion Buthus martensi Karsch (BmK). We found that the expression of P2X7 receptors (P2X7Rs) was up-regulated in the ipsilateral spinal dorsal horn after BmK I injection in rats. P2X7R was selectively localized in microglia but not astrocytes or neurons.

Neuroprotective effects of a Rhodiola crenulata extract on amyloid-β peptides (Aβ) -induced cognitive deficits in rat models of Alzheimer's disease.

Background: Rhodiola crenulata has been wildly used as a healthy food, antidepressant and antifatigue for many years in China. Recent studies suggested that Rhodiola crenulata extract (RCE) has cognitive protective effects in the treatment of Alzheimer's disease (AD).

Purpose: To assess the protective effects of RCE on cognitive deficits and clarify its therapeutic mechanisms in Aβ -induced rat models of AD.

Cetuximab-modified silica nanoparticle loaded with ICG for tumor-targeted combinational therapy of breast cancer.

Combinational therapy is usually considered as a preferable approach for effective cancer therapy. Especially, combinational chemo and photothermal therapy is of particular interest due to its high flexibility as well as efficiency. In this article, we the silica nanoparticles (SLN) were surface conjugated with Cetuximab (Cet-SLN) to target epidermal growth factor receptor (EGFR), a common receptor that usually observed to overexpress in multiple breast cancers.

A metabolomic study based on accurate mass and isotopic fine structures by dual mode combined-FT-ICR-MS to explore the effects of Rhodiola crenulata extract on Alzheimer disease in rats.

A metabolomic strategy based on accurate mass and isotopic fine structures (IFSs) by dual mode combined-Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) was established to explore the effects of Rhodiola crenulata extract (RCE) on Alzheimer disease (AD) in rats. Experimental AD model was induced in rats by bilateral hippocampal injection of Aβ, and Morris water maze task (MWM) was used to evaluate the effects of RCE on AD. Subsequently, the metabolomic study was performed using HPLC-FT-ICR-MS, fraction collector and direct infusion (DI)-FT-ICR-MS to screen and identify the potential biomarkers.

Long non-coding RNA LOC285194 functions as a tumor suppressor by targeting p53 in non-small cell lung cancer.

Recently, LOC285194 has shown a potential tumor-suppressor function in several types of human cancers, but its function in non-small cell lung cancer (NSCLC) remains unknown. This study intended to investigate the biological role of LOC285194 and its clinical significance in NSCLC. LOC285194 was detected by qRT-PCR, and its correlation with clinicopathological features of NSCLC was analyzed.

MicroRNAs and their targets in cucumber shoot apices in response to temperature and photoperiod.

Background: The cucumber is one of the most important vegetables worldwide and is used as a research model for study of phloem transport, sex determination and temperature-photoperiod physiology. The shoot apex is the most important plant tissue in which the cell fate and organ meristems have been determined. In this study, a series of whole-genome small RNA, degradome and transcriptome analyses were performed on cucumber shoot apical tissues treated with high vs.

An available strategy for elemental composition determination of organic impurities in commercial preparations based on accurate mass and peak ratio of isotopic fine structures (IFSs) by dual mode combined-FT-ICR-MS and fraction collection technology.

A strategy to determine the elemental composition of organic impurities in commercial preparations was established by combining high-performance liquid chromatography (HPLC)-Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and fraction collector with direct infusion-FT-ICR-MS (DI-FT-ICR-MS). Under HPLC-FT-ICR-MS mode, all impurities in model sample were chromatographically separated and collected by fraction collector according to their retention time. The accurate mass of each impurity and their candidate formulae under 1 ppm mass tolerance were calculated.

Microarray analysis reveals long non‑coding RNA SOX2OT as a novel candidate regulator in diabetic nephropathy.

Diabetic nephropathy (DN) is a highly complex syndrome involving multiple dysregulated biological processes. Long non‑coding RNAs (lncRNAs) are now believed to have an important function in various diseases. However, their roles in DN remain largely unknown.

Inhibition of microRNA-182-5p contributes to attenuation of lupus nephritis via Foxo1 signaling.

MiR-182-5p suppresses expression of Foxo1 that is a protective factor in renal disorders and is up-regulated in systemic lupus erythematosus patients. Thus, we hypothesized that dys-function of miR-182-5p/Foxo1 axis contributed to development of lupus nephritis (LN). Firstly, we investigated the expressions of miR-182-5p and Foxo1 in LN patients and during growth of LN MRL/lpr mice.

A Descriptive, Cross-sectional Study Among Chinese Patients to Identify Factors that Affect Psychosocial Adjustment to an Enterostomy.

Physiological, psychological, and social problems may affect adaptation to living with a stoma. A descriptive, cross-sectional study was conducted between March 2017 and June 2017 among patients culled from a manufacturer's database to identify factors that influence psychosocial adjustment in Chinese patients with an enterostoma. Patients with a history of ostomy surgery ≥1 month prior and who were ≥18 years of age, completed a primary school education, and able to communicate in Chinese were eligible to participate unless they had a history of psychosis, cognitive impairment, or participation in other research programs.

Derivation and Validation of Risk Scores to Predict Cerebrovascular Mortality Among Incident Peritoneal Dialysis Patients.

Background/aims: Cerebrovascular disease (CeVD) is one of the leading causes of death in patients initialising peritoneal dialysis (PD). Currently there is no risk score to predict the future risk of CeVD on entry into PD. This study aimed to derive and validate a risk prediction model for CeVD mortality in 2 years after the initialisation of PD.