O-GlcNAcylation of RPA2 at S4/S8 antagonizes phosphorylation and regulates checkpoint activation during replication stress.

O-linked N-acetylglucosamine (O-GlcNAc) is the most abundant mono-saccharide modification occurring in the cytoplasm, nucleus, and mitochondria. The recent advent of mass spectrometry technology has enabled the identification of abundant O-GlcNAc transferase (OGT) substrates in diverse biological processes, such as cell cycle progression, replication, and DNA damage response. Herein we report the O-GlcNAcylation of Replication Protein A2 (RPA2), a component of the heterotrimeric RPA complex pivotal for DNA metabolism.

Synthesis of glucosamine-selenium compound and evaluation of its oral toxicity and in vitro anti-hepatitis B virus activity.

Selenium supplements are beneficial to human health, however, concerns regarding the toxicity of inorganic selenium have stimulated research on safer organic compounds. The main objective of this study was to develop a novel glucosamine-selenium compound (Se-GlcN), clarify its structure, and subsequently investigate its oral toxicity and in vitro anti-hepatitis B virus (HBV) activity. Electron microscopy, infrared, ultraviolet spectroscopy, nuclear magnetic resonance and thermogravimetric analyses revealed a unique binding mode of Se-GlcN, with the introduction of the Se-O bond at the C6 position, resulting in the formation of two carboxyl groups.

ETV2 regulating PHD2-HIF-1α axis controls metabolism reprogramming promotes vascularized bone regeneration.

The synchronized development of mineralized bone and blood vessels is a fundamental requirement for successful bone tissue regeneration. Adequate energy production forms the cornerstone supporting new bone formation. ETS variant 2 (ETV2) has been identified as a transcription factor that promotes energy metabolism reprogramming and facilitates the coordination between osteogenesis and angiogenesis.

Interaction of Macrophages with Bone Healing Microenvironment: Mechanism and Biomaterials.

Extensive bone fractures, which can seriously impact both health and quality of life, cannot easily heal naturally, especially if the patient has an underlying medical condition or is aging. The most promising approach to addressing such fractures is bone regeneration through bone tissue engineering. Bone regeneration is a complex process that consists of three distinct phases: inflammation, repair, and remodeling.

Comparison of chemotherapy regimens plus rituximab in adult Burkitt lymphoma: A single-arm meta-analysis.

Background And Aim: Given the paucity of evidence-based treatment recommendations, the most appropriate first-line regimen for adult Burkitt lymphoma is currently undefined. We aimed to identify the optimal treatment regimen containing rituximab for adult Burkitt lymphoma patients.

Methods: The PubMed, Embase, Web of Science, and Cochrane databases were searched in December 2021 (10).

Unleashing the dynamic and nonlinear relationship among new-type urbanization, foreign direct investment, and inclusive green growth in China: an environmental sustainability perspective.

As an important way for China to integrate into the international circulation, foreign direct investment (FDI) can not only increase China's capital accumulation but also directly affect inclusive green growth by promoting the diffusion and transfer of green technologies and activating the domestic market. Based on China's provincial panel data from 2007 to 2019, this paper discusses the following issues: first, Global Malmquist-Luenberger (GML) productivity index is used to measure and analyze the current situation of inclusive green growth in 30 provinces of China; second, we set a fixed effect panel model to test the relationship between FDI and inclusive green growth in China. Third, based on PVAR (panel vector autoregressive) model, the dynamic impacts of FDI and new urbanization on inclusive green growth are tested.

The characteristics and prognostic significance of the SET-CAN/NUP214 fusion gene in hematological malignancies: A systematic review.

Background: The SET-CAN/NUP214 fusion gene resulting from chromosomal del(9)(q34.11q34.13) or t(9;9) (q34;q34) has been found in T-cell acute lymphoblastic leukemia (T-ALL), B-cell acute lymphoblastic leukemia (B-ALL), acute myeloid leukemia (AML) and myeloid sarcoma (MS).

The involvement of oxidative stress, neuronal lesions, neurotransmission impairment, and neuroinflammation in acrylamide-induced neurotoxicity in C57/BL6 mice.

Acrylamide (ACR) is a typical environmental contaminant, presenting potential health hazards that have been attracting increasing attention. Its neurotoxicity is known to cause significant damage to health. However, the mechanisms of ACR-induced neurotoxicity require further clarification.

Multifunctional Coatings of Titanium Implants Toward Promoting Osseointegration and Preventing Infection: Recent Developments.

Titanium and its alloys are dominant material for orthopedic/dental implants due to their stable chemical properties and good biocompatibility. However, aseptic loosening and peri-implant infection remain problems that may lead to implant removal eventually. The ideal orthopedic implant should possess both osteogenic and antibacterial properties and do proper assistance to inflammatory cells for anti-microbe and tissue repair.

Polarized Macrophages in Periodontitis: Characteristics, Function, and Molecular Signaling.

Periodontitis (PD) is a common chronic infectious disease. The local inflammatory response in the host may cause the destruction of supporting periodontal tissue. Macrophages play a variety of roles in PD, including regulatory and phagocytosis.

Case report: Rare myeloid sarcoma development following renal transplantation with KRAS and DNMT3A gene mutations.

Background: A high incidence of malignant tumors, such as post-transplant lymphoproliferative disorders (PTLD), Kaposi sarcoma, and renal cancer is common in solid organ and bone marrow transplant recipients. However, myeloid sarcoma (MS) after renal transplantation has rarely been reported and the diagnosis is challenging due to its low incidence.

Case Presentation: Here, we report a rare case of a 49-year-old man who developed myeloid sarcoma (MS) in the transplanted kidney two years after renal transplantation.

Poly-l-lysine/Sodium Alginate Coating Loading Nanosilver for Improving the Antibacterial Effect and Inducing Mineralization of Dental Implants.

In recent years, antibacterial surface modification of titanium (Ti) implants has been widely studied in preventing implant-associated infection for dental and orthopedic applications. The purpose of this study was to prepare a composite coating on a porous titanium surface for infection prevention and inducing mineralization, which was initialized by deposition of a poly--lysine (PLL)/sodium alginate(SA)/PLL self-assembled coating, followed by dopamine deposition, and finally in situ reduction of silver nanoparticles (AgNPs) by dopamine. The surface zeta potential, SEM, XPS, UV-vis, and water contact angle analyses demonstrate that each coating was successfully prepared after the respective steps and that the average sizes of AgNPs were 20-30 nm.

A Temporal Adaptive Access Mechanism for Data Fusion in an IoT Environment.

Data fusion in the Internet of Things (IoT) environment demands collecting and processing a wide variety of data with mixed time characteristics, both real-time and non-real-time data. Most of the previous research on data fusion was about the data processing aspect; however, successful data transmission is a prerequisite for high-performance data fusion in IoT. On the other hand, research on data transmissions in IoT mainly focuses on networking without sufficiently considering the special requirements of the upper-layer applications, such as the data fusion process, that are consuming the transmitted data.

[Carbon Metabolism Characteristics of the Karst Soil Microbial Community for Pb-Zn Mine Tailings].

BIOLOG and 18S rRNA PCR-DGGE methods were used to estimate the characteristics of carbon source metabolism of the soil microbial community as well as the relationship between soil fungi and soil organic carbon in different karst land use types (corn field, citrus field, and paddy field) contaminated by Pb-Zn tailings at Sidi Village, Yangshuo, Guangxi Zhuang Autonomous Region, SW China. It was found that the concentrations of Pb, Zn, Cu, and Cd were highest in the paddy field, followed by the corn field, citrus field, and control group (dry field). In addition, the geo-accumulation index indicated that the heavy metal pollution at this area was caused by Pb and Cd and that Cd was the key environmental risk factor.

[Water Chemistry and Characteristics of Dissolved Organic Carbon During the Wet Season in Wulixia Reservoir, SW China].

In recent years, scientists have focused on the karst carbon cycle. To better understand the hydrochemical characteristics and the physical-chemical properties of DOC in the wet season in karst areas, the water chemistry and DOC distribution characteristics in Wulixia reservoir were analyzed. The molecular weight of the water organic matter was analyzed based on the UV absorption spectrum.

[Distinguishing the Properties and Sources of the Dissolved Organic Matter in Karst Reservoir Water During Winter Using Three-Dimensional Fluorescence Spectrum Technology:A Case Study in Wulixia Reservoir of Guangxi Province].

In recent years, the fluorescence spectrum technology has been widely applied to study the physical and chemical properties of dissolved organic matter (DOM) in natural water. In order to reveal the properties of the dissolved organic matter in reservoir water, the three-dimensional fluorescence spectrum and the parallel factor analysis were used to determine the properties of DOM and its sources in the Wulixia reservoir during the winter of 2016. Moreover, the chemical characteristics of water and the relative proportion of DOM fluorescent components were estimated.

Facile Soaking Strategy Toward Simultaneously Enhanced Conductivity and Toughness of Self-Healing Composite Hydrogels Through Constructing Multiple Noncovalent Interactions.

Tough and stretchable conductive hydrogels are desirable for the emerging field of wearable and implanted electronics. Unfortunately, most existing conductive hydrogels have low mechanical strength. Current strategies to enhance mechanical properties include employing tough host gel matrices or introducing specific interaction between conductive polymer and host gel matrices.

BlueDetect: An iBeacon-Enabled Scheme for Accurate and Energy-Efficient Indoor-Outdoor Detection and Seamless Location-Based Service.

The location and contextual status (indoor or outdoor) is fundamental and critical information for upper-layer applications, such as activity recognition and location-based services (LBS) for individuals. In addition, optimizations of building management systems (BMS), such as the pre-cooling or heating process of the air-conditioning system according to the human traffic entering or exiting a building, can utilize the information, as well. The emerging mobile devices, which are equipped with various sensors, become a feasible and flexible platform to perform indoor-outdoor (IO) detection.

Robust Extreme Learning Machine With its Application to Indoor Positioning.

The increasing demands of location-based services have spurred the rapid development of indoor positioning system and indoor localization system interchangeably (IPSs). However, the performance of IPSs suffers from noisy measurements. In this paper, two kinds of robust extreme learning machines (RELMs), corresponding to the close-to-mean constraint, and the small-residual constraint, have been proposed to address the issue of noisy measurements in IPSs.

A fast and precise indoor localization algorithm based on an online sequential extreme learning machine.

Nowadays, developing indoor positioning systems (IPSs) has become an attractive research topic due to the increasing demands on location-based service (LBS) in indoor environments. WiFi technology has been studied and explored to provide indoor positioning service for years in view of the wide deployment and availability of existing WiFi infrastructures in indoor environments. A large body of WiFi-based IPSs adopt fingerprinting approaches for localization.

[Cloning and sequencing of Sry gene of muntiacus].

Using the primers from SRY gene--HMG Box for PCR amplification in genomic DNA of Muntiacus reevesi cell strains, a 220bp fragment was obtained in the male but not in the female. The 220bp fragment was cloned into the pGEM-T vector using T/A clone method. The identified positive clone was sequenced.

[Construction and screening of hepatitis E virus-specific phage antibody combinatorial library].

Aim: To construct HEV-specific phage combinatorial anti-body library and screen anti-HEV antibodies with neutralizing activity from the library.

Methods: The total RNA was extracted from B-lymphocytes of 6 HE patients. Kappa chain and Fd segment of IgG gene were amplified respectively by RT-PCR using a set of Fab-specific primers.