Generation of an Aptamer Targeting Receptor-Type Tyrosine-Protein Phosphatase F.

Cell-SELEX is a powerful tool to generate aptamers that specifically bind the native molecules on living cells. Here, we report an aptamer ZAJ4a generated by cell-SELEX. The molecular target of ZAJ4a was pulled down by the enriched cell-SELEX pool and identified to be the receptor-type tyrosine-protein phosphatase F (PTPRF) through a stable isotope labeling using amino acids in cell culture (SILAC)-based quantitative proteomic method.

Characterization and Identification of Aptamers against CD49c for the Detection, Capture, and Release of Cancer Cells.

As a kind of recognition molecule, aptamers can be inserted into some regulatory sequences for the smart response of their targets. However, the molecular engineering might lead to the change of the binding affinity. Here, we present a stable aptamer ZAJ-2c and an environmentally sensitive aptamer ZAJ-2d optimized from an original cell-binding aptamer ZAJ-2, and the molecular target was further identified as CD49c on the cell membrane.

Depression Speech Recognition With a Three-Dimensional Convolutional Network.

Depression has become one of the main afflictions that threaten people's mental health. However, the current traditional diagnosis methods have certain limitations, so it is necessary to find a method of objective evaluation of depression based on intelligent technology to assist in the early diagnosis and treatment of patients. Because the abnormal speech features of patients with depression are related to their mental state to some extent, it is valuable to use speech acoustic features as objective indicators for the diagnosis of depression.

Rapid Detection of Single Viable O157 Cells in Fresh Lettuce and Strawberry by Immunomagnetic Flow Cytometry in Combination with Pre-Enrichment.

Enterohemorrhagic are an important pathogen causing food poisoning. The rapid detection of viable O157 in vegetables and fruits at single-cell level is critical because of the low infective dose of this pathogen. In this study, an immunomagnetic flow cytometry (IMFC)-based method was developed to detect O157 in lettuce and strawberries inoculated with 1 CFU/25 g.

Faster Detection of in Milk and Milk Powder by Flow Cytometry.

A flow cytometry (FCM)-based method was developed for the faster detection of in milk and milk powder. Viable cells were recognized by highly selective, fluorescently labeled antibodies and Propidium Iodide, and then analyzed by FCM. Using a 5-h pre-enrichment period, the method could detect low numbers of cells in 6 h, with a limit of detection of 7.

Detection of Circulating Tumor-Related Materials by Aptamer Capturing and Endogenous Enzyme-Signal Amplification.

Circulating tumor-related materials (CTRMs) shed from original or metastatic tumors, carry a lot of tumor information and are considered as important markers for cancer diagnosis and metastasis prognosis. Herein, we report a colorimetric detection strategy for CTRMs based on aptamer-based magnetic isolation and endogenous alkaline phosphatase (AP)-signal amplification. This strategy exhibited high sensitivity and selectivity toward the CTRMs that express AP heterodimers (the target of aptamer, a potential tumor marker).