A Soothing Lavender-Scented Electrospun Fibrous Eye Mask.

Electrospinning technology has demonstrated extensive applications in biomedical engineering, energy storage, and environmental remediation. However, its utilization in the cosmetic industry remains relatively underexplored. To address the challenges associated with skin damage caused by preservatives and thickeners used for extending the shelf life of conventional products, a soothing lavender-scented electrospun fibrous eye mask with coaxial layers was developed using the electrospinning technique.

EnzyACT: A Novel Deep Learning Method to Predict the Impacts of Single and Multiple Mutations on Enzyme Activity.

Enzyme engineering involves the customization of enzymes by introducing mutations to expand the application scope of natural enzymes. One limitation of that is the complex interaction between two key properties, activity and stability, where the enhancement of one often leads to the reduction of the other, also called the trade-off mechanism. Although dozens of methods that predict the change of protein stability upon mutations have been developed, the prediction of the effect on activity is still in its early stage.

Rational Design of Flexible, Self-Supporting, and Binder-Free Prussian White/KetjenBlack/MXene Composite Electrode for Sodium-Ion Batteries with Boosted Electrochemical Performance.

Due to their cost-effectiveness, abundant resources, and suitable working potential, sodium-ion batteries are anticipated to establish themselves as a leading technology in the realm of grid energy storage. However, sodium-ion batteries still encounter challenges, including issues related to low energy density and constrained cycling performance. In this study, a self-supported electrode composed of Prussian white/KetjenBlack/MXene (TK-PW) is proposed.

Research on Circuit Breaker Operating Mechanism Fault Diagnosis Method Combining Global-Local Feature Extraction and KELM.

In response to the lack of generality in feature extraction using modal decomposition methods and the susceptibility of diagnostic performance to parameter selection in traditional mechanical fault diagnosis of high-voltage circuit breaker operating mechanisms, this paper proposes a Global-Local feature extraction method based on Generalized S-Transform (S-Translate) combined with Gray Level Co-Occurrence Matrix (GLCM) and complemented by Maximum Relevance and Minimum Redundancy (mRMR) feature selection. The GL (Global-Local)-mRMR-KELM fault diagnosis model is proposed, which employs the Kernel Extreme Learning Machine (KELM). In this model, the original time-frequency domain features and the time-frequency features of the Generalized S-Transform matrix of vibration signals under different states of the circuit breaker are first extracted as global features.

The mechanism and targeted intervention of the HIF-1 pathway in improving atherosclerotic heart's sensitivity to ischemic postconditioning.

Background: IPoC possesses a preventive effect against IR injury in healthy myocardium, but IPoC's protective effect on atherosclerotic myocardium is controversial. The current investigation aims to determine whether IPoC remains protective in atherosclerotic myocardium subjected to ischemia-reperfusion (IR) injury; to explore the specific mechanisms by which IPoC exerts cardioprotection; to explore whether HIF-1 upregulation combined with IPoC could further the provide cardioprotection; and to gaze at the specific mechanism whereby combined treatment expert the cardioprotection.

Methods: ApoE mice fed with a high-fat diet (HFD) were used to develop a model of atherosclerosis.

An Interpretable Multitask Framework BiLAT Enables Accurate Prediction of Cyclin-Dependent Protein Kinase Inhibitors.

The cyclin-dependent protein kinases (CDKs) are protein-serine/threonine kinases with crucial effects on the regulation of cell cycle and transcription. CDKs can be a hallmark of cancer since their excessive expression could lead to impaired cell proliferation. However, the selectivity profile of most developed CDK inhibitors is not enough, which have hindered the therapeutic use of CDK inhibitors.

KID: A Kinase-Focused Interaction Database and Its Application in the Construction of Kinase-Focused Molecule Databases.

Protein kinases are important drug targets for the treatment of several diseases. The interaction between kinases and ligands is vital in the process of small-molecule kinase inhibitor (SMKI) design. In this study, we propose a method to extract fragments and amino acid residues from crystal structures for kinase-ligand interactions.

FSDscore: An Effective Target-focused Scoring Criterion for Virtual Screening.

Improving screening efficiency is one of the most challenging tasks of virtual screening (VS). In this work, we propose an effective target-focused scoring criterion for VS and apply it to the screening of a specific target scaffold replacement library constructed by enumeration of suitable substitution fragments and R-groups of known ligands. This criterion is based on both ligand- and structure-based scoring methods, which includes feature maps, 3D shape similarity, and the pairwise distance information between proteins and ligands (FSDscore).

Household Groups' Land Use Decisions Investigation Based on Perspective of Livelihood Heterogeneity in Sichuan Province, China.

Land use decision-making is a vital livelihood strategy associated with the rational collocation of livelihood asset endowments by rural households. Based on the perspective of livelihood heterogeneity, this paper collected the data from 540 farm households in 27 villages in three Sichuan Province counties to identify the land use decision-making characteristics of the household groups. A land use decision-making framework (LUDF) based on the sustainable livelihood framework (SLF) was established and dynamic and dual indicators were developed to divide the sample into six household groups.

Research on the Influence of Informal Employment on Residents' Happiness in China: Empirical Analysis Based on CLDS Data.

The influence of informal employment on residents' happiness has gained wide attention around the world. However, few studies focus on this topic in China. Using the 2016 wave of the China Labor Force Dynamics Survey (CLDS) data, we examined the effect of informal employment and its mechanisms on residents' happiness in China.

Age, male sex, higher posterior tibial slope, deep sulcus sign, bone bruises on the lateral femoral condyle, and concomitant medial meniscal tears are risk factors for lateral meniscal posterior root tears: a systematic review and meta-analysis.

Purpose: Lateral meniscus posterior root tears (LMPRTs) are commonly found in patients with anterior cruciate ligament (ACL) injuries. However, risk factors for LMPRTs are not well known. This study was designed to systematically review the available evidence regarding risk factors associated with LMPRTs.

Effective Reaction-Based Strategy for Kinase Targets: A Case Study on MERTK Inhibitors.

Reaction-based design is the computational generation of novel molecular structures by linking building blocks using reaction vectors derived from chemistry knowledge. In this work, we first adopted a recurrent neural network (RNN) model to generate three groups of building blocks with different functional groups and then constructed an in silico target-focused combinatorial library based on chemical reaction rules. Mer tyrosine kinase (MERTK) was used as a study case.

Drug repositioning: Progress and challenges in drug discovery for various diseases.

Compared with traditional de novo drug discovery, drug repurposing has become an attractive drug discovery strategy due to its low-cost and high efficiency. Through a comprehensive analysis of the candidates that have been identified with drug repositioning potentials, it is found that although some drugs do not show obvious advantages in the original indications, they may exert more obvious effects in other diseases. In addition, some drugs have a synergistic effect to exert better clinical efficacy if used in combination.

Minimum distance quantile regression for spatial autoregressive panel data models with fixed effects.

This paper considers the quantile regression model with individual fixed effects for spatial panel data. Efficient minimum distance quantile regression estimators based on instrumental variable (IV) method are proposed for parameter estimation. The proposed estimator is computational fast compared with the IV-FEQR estimator proposed by Dai et al.

Quantile regression for general spatial panel data models with fixed effects.

This paper considers the quantile regression model with both individual fixed effect and time period effect for general spatial panel data. Fixed effects quantile regression estimators based on instrumental variable method will be proposed. Asymptotic properties of the proposed estimators will be developed.

[Pseudoangiosarcomatous squamous cell carcinoma of the penis: a case report with clinicopathological and human papilloma virus analyses].

Objective: To further understand the clinicopathological, ultrastructural and molecular features of penile pseudoangiosarcomatous squamous cell carcinoma (PASCC), and improve its diagnosis and treatment.

Methods: A 47-year-old male patient with penile PASCC was reported and the relevant literature reviewed. The main clinical manifestations of the patient were a typical surface ulceration with hemorrhage and purulent secretion with a foul smell, a papillary mass about 5.

Direct visualization of reversible switching of micropatterned polyelectrolyte brushes on gold surfaces using laser scanning confocal microscopy.

We apply confocal fluorescence microscopy for real time studies of reversible conformational changes of poly(methacryloyloxyethyl phosphate) (PMEP) brushes chemically grafted onto gold substrates. Oregon green 488 fluorophores chemically attached onto the PMEP polymers were used as reporters for probing the conformational changes. Use of a specially designed liquid flow microchamber allowed dynamic imaging of the brushes under varying environmental conditions.

Fluorescence intensity and lifetime imaging of free and micellar-encapsulated doxorubicin in living cells.

Frequency domain fluorescence lifetime imaging microscopy (FLIM) has been used in combination with laser scanning confocal microscopy to study the cellular uptake behavior of the antitumor drug doxorubicin (DOX) and micellar-encapsulated DOX (PLyAd-DOX). The endocytosis uptake process of PLyAd-DOX was monitored over 72 hours using confocal microscopy, with a maximum fluorescence recorded at incubation periods around 24 hours. The micellar structure was not found to release the encapsulated DOX during the time course of imaging.

[Expression of epithelial-cadherin, CD44v6 and connexin43 in hepatocellular carcinoma].

Objective: To study the expression of epithelial-cadherin (E-cad), CD44v6 and Cx43 in hepatocellular carcinoma (HCC) and its relationship with sex and age of patients, as well as tumor histopathologic grades.

Methods: Double immunofluorescent staining and laser scanning confocal microscopy was used to study the expression of E-cad, CD44v6 and Cx43 in 30 cases of normal liver tissue, 25 cases of benign hepatic lesions and 38 cases of HCC. In the HCC group, correlation of antigen expression with sex and age of patients and tumor histopathologic grades was studied by T-test.

[Expression of integrin-linked kinase in prostate cancer and its significance].

Objective: To investigate the expression of integrin-linked kinase (ILK) in primary prostate cancer and its clinical significance.

Methods: The expression of ILK was analysed in 50 prostate cancer and 16 benign prostatic hyperplasia samples by immunohistochemical staining.

Results: The positive percentage of ILK was 46.

Pathological characteristics of gastric leiomyoblastoma.

Aim: To determine the pathological characteristics of gastric leiomyoblastoma.

Methods: All tissues were obtained during surgery or gastroscopy. Tissue specimens for examination by light microscope were 1 cm x 1 cm x 1 cm in size, fixed in 40 g/L neutral buffered formaldehyde, embedded in paraffin, and stained with hematoxylin and eosin.

Expressions of chromogranin A and cathepsin D in human primary hepatocellular carcinoma.

AIM:To determine the expression and clinical significance of chromogranin A and cathepsin D in hepatocellular carcinoma (HCC).METHODS:Double immunofluorescence stain-ing techniques combined with laser confocal scanning microscopy (LSCM) was used to investigate chromogranin A and cathepsin D expressions in 85 HCC patients.RESULTS:Cathepsin D was expressed in 3 normal liver tissues, while in HCC the staining showed regional variation and the fraction of strongly stained cells increased as the tumors became less differentiated and usually clinically more malignant.