53BP1, a known chromatin-associated factor that promotes DNA damage repair, is differentially modulated during bovine herpesvirus 1 infection in vitro and in vivo.

Bovine herpesvirus 1 (BoHV-1) productive infection induces the formation of DNA double-strand breaks (DSBs), the most severe form of DNA lesions in cultured cells. 53BP1, a chromatin-associated factor, plays an essential role in DNA damage repair. In this study, we demonstrated that BoHV-1 productive infection in bovine kidney (MDBK) cells increased the expression of phosphorylated form of H2AX protein (γH2AX) and promoted the formation of γH2AX foci in the nucleus, indicative of enhanced DNA lesions.

Application of Four-Dimensional Pelvic Floor Ultrasound in the Diagnosis of Postpartum Pelvic Floor Dysfunction and Evaluation of Curative Effect.

Objective: To explore the application of four-dimensional pelvic floor ultrasound in the diagnosis of postpartum pelvic floor dysfunction (PFD) and evaluation of curative effect.

Methods: A total of 100 patients with postpartum PFD undergoing vaginal delivery in the hospital were enrolled as the research objects between January 2020 and January 2023. A total of 100 postpartum women with good pelvic floor muscle function during the same period were enrolled as a control group.

Real-Time Belt Deviation Detection Method Based on Depth Edge Feature and Gradient Constraint.

Aiming at the problems of the poor recognition effect and low recognition rate of the existing methods in the process of belt deviation detection, this paper proposes a real-time belt deviation detection method. Firstly, ResNet18 combined with the attention mechanism module is used as a feature extraction network to enhance the features in the belt edge region and suppress the features in other regions. Then, the extracted features are used to predict the approximate locations of the belt edges using a classifier based on the contextual information on the fully connected layer.

Single-path electro-optic coefficients measurement approach using multiple reflection interference.

An electro-optic coefficient (EOC) is an important optical property of optical crystals. In this work, a single-path multiple reflection interferometer is proposed to measure the components of signed EOCs with high precision. With the help of the normalization method, the influence of incident light was removed, and repeatability of the experimental results was improved.

Circular RNA circRHOBTB3 represses metastasis by regulating the HuR-mediated mRNA stability of PTBP1 in colorectal cancer.

Tumor metastasis of colorectal cancer (CRC) is the main cause of death in most patients and the major difficulty in comprehensive CRC treatment. Circular RNAs (circRNAs) affect many biological functions in solid tumors. However, their mechanisms in CRC metastasis remain unclear.

Bovine Herpesvirus 1 Productive Infection Led to Inactivation of Nrf2 Signaling through Diverse Approaches.

Bovine herpesvirus type 1 (BoHV-1) is a significant cofactor for bovine respiratory disease complex (BRDC), the most important inflammatory disease in cattle. BoHV-1 infection in cell cultures induces overproduction of pathogenic reactive oxygen species (ROS) and the depletion of nuclear factor erythroid 2 p45-related factor 2 (Nrf2), a master transcriptional factor regulating a panel of antioxidant and cellular defense genes in response to oxidative stress. In this study, we reported that the virus productive infection in MDBK cells at the later stage significantly decreased the expression levels of heme oxygenase-1 (HO-1) and NAD(P)H quinone oxidoreductase-1 (NQO1) proteins, the canonical downstream targets regulated by Nrf2, inhibited Nrf2 acetylation, reduced the accumulation of Nrf2 proteins in the nucleus, and relocalized nuclear Nrf2 proteins to form dot-like staining patterns in confocal microscope assay.

The Differential Expression of Mitochondrial Function-Associated Proteins and Antioxidant Enzymes during Bovine Herpesvirus 1 Infection: A Potential Mechanism for Virus Infection-Induced Oxidative Mitochondrial Dysfunction.

Reactive oxidative species (ROS) are important inflammatory mediators. Electrons escaping from the mitochondrial electron transport chain (ETC) during oxidative phosphorylation (OXPHOS) in the mitochondrial respiratory chain (RC) complexes contribute to ROS production. The cellular antioxidant enzymes are important for maintaining ROS release at the physiological levels.

The Involvement of Histone H3 Acetylation in Bovine Herpesvirus 1 Replication in MDBK Cells.

During bovine herpesvirus 1 (BoHV-1) productive infection in cell cultures, partial of intranuclear viral DNA is present in nucleosomes, and viral protein VP22 associates with histones and decreases histone H4 acetylation, indicating the involvement of histone H4 acetylation in virus replication. In this study, we demonstrated that BoHV-1 infection at the late stage (at 24 h after infection) dramatically decreased histone H3 acetylation [at residues K9 (H3K9ac) and K18 (H3K18ac)], which was supported by the pronounced depletion of histone acetyltransferases (HATs) including CBP/P300 (CREB binding protein and p300), GCN5L2 (general control of amino acid synthesis yeast homolog like 2) and PCAF (P300/CBP-associated factor). The depletion of GCN5L2 promoted by virus infection was partially mediated by ubiquitin-proteasome pathway.

Induction of Oxidative DNA Damage in Bovine Herpesvirus 1 Infected Bovine Kidney Cells (MDBK Cells) and Human Tumor Cells (A549 Cells and U2OS Cells).

Bovine herpesvirus 1 (BoHV-1) is an important pathogen of cattle that causes lesions in mucosal surfaces, genital tracts and nervous systems. As a novel oncolytic virus, BoHV-1 infects and kills numerous human tumor cells. However, the mechanisms underlying the virus-induced cell damages are not fully understood.

Managing Scarce Water Resources in China's Coal Power Industry.

Coal power generation capacity is expanding rapidly in the arid northwest regions in China. Its impact on water resources is attracting growing concerns from policy-makers, researchers, as well as mass media. This paper briefly describes the situation of electricity-water conflict in China and provides a comprehensive review on a variety of water resources management policies in China's coal power industry.

Revealing Water Stress by the Thermal Power Industry in China Based on a High Spatial Resolution Water Withdrawal and Consumption Inventory.

This study reveals the spatial distribution of water withdrawal and consumption by thermal power generation and the associated water stress at catchment level in China based on a high-resolution geodatabase of electric generating units and power plants. We identified three groups of regions where the baseline water stress exerted by thermal power generation is comparatively significant: (1) the Hai River Basin/East Yellow River Basin in the north; (2) some arid catchments in Xinjiang Autonomous Region in the northwest; and (3) the coastal city clusters in the Yangtze River Delta, Pearly River Delta, and Zhejiang Province. Groundwater stress is also detected singularly in a few aquifers mainly in the Hai River Basin and the lower reaches of the Yellow River Basin.