Measurement and analysis of photoacoustic pressure induced by weak microsecond pulsed light.

The photoacoustic effect (PAE) of weak microsecond pulsed light (WMPL) has immense potential for application to biomedical engineering. However, practical measurements and theoretical analysis of the photoacoustic pressure of WMPL are lacking. Hence, we constructed a WMPL photoacoustic pressure measurement system using an electret piezoelectric sensor and multi-wavelength parameter-adjustable pulsed light generator.

Trends and Influencing Factors in Problematic Smartphone Use Prevalence (2012-2022): A Systematic Review and Meta-Analysis.

Given that problematic smartphone use (PSU) has been researched for a long time, it becomes necessary to assess how the trends of PSU prevalence have evolved over time. In total, 106 articles from 2012 to 2022 with 109 studies covering 97,748 individuals were included in this systematic review and meta-analysis. The estimation of the global pooled PSU prevalence was 37.

An ECG Signal Denoising Method Using Conditional Generative Adversarial Net.

In this paper, a novel denoising method for electrocardiogram (ECG) signal is proposed to improve performance and availability under multiple noise cases. The method is based on the framework of conditional generative adversarial network (CGAN), and we improved the CGAN framework for ECG denoising. The proposed framework consists of two networks: a generator that is composed of the optimized convolutional auto-encoder (CAE) and a discriminator that is composed of four convolution layers and one full connection layer.

In-depth phosphoproteomic analysis of royal jelly derived from western and eastern honeybee species.

The proteins in royal jelly (RJ) play a pivotal role in the nutrition, immune defense, and cast determination of honeybee larvae and have a wide range of pharmacological and health-promoting functions for humans as well. Although the importance of post-translational modifications (PTMs) in protein function is known, investigation of protein phosphorylation of RJ proteins is still very limited. To this end, two complementary phosphopeptide enrichment materials (Ti(4+)-IMAC and TiO2) and high-sensitivity mass spectrometry were applied to establish a detailed phosphoproteome map and to qualitatively and quantitatively compare the phosphoproteomes of RJ produced by Apis mellifera ligustica (Aml) and Apis cerana cerana (Acc).

In-depth proteomics characterization of embryogenesis of the honey bee worker (Apis mellifera ligustica).

Identifying proteome changes of honey bee embryogenesis is of prime importance for unraveling the molecular mechanisms that they underlie. However, many proteomic changes during the embryonic period are not well characterized. We analyzed the proteomic alterations over the complete time course of honey bee worker embryogenesis at 24, 48, and 72 h of age, using mass spectrometry-based proteomics, label-free quantitation, and bioinformatics.

Comprehensive identification of novel proteins and N-glycosylation sites in royal jelly.

Background: Royal jelly (RJ) is a proteinaceous secretion produced from the hypopharyngeal and mandibular glands of nurse bees. It plays vital roles in honeybee biology and in the improvement of human health. However, some proteins remain unknown in RJ, and mapping N-glycosylation modification sites on RJ proteins demands further investigation.

Proteome and phosphoproteome analysis of honeybee (Apis mellifera) venom collected from electrical stimulation and manual extraction of the venom gland.

Background: Honeybee venom is a complicated defensive toxin that has a wide range of pharmacologically active compounds. Some of these compounds are useful for human therapeutics. There are two major forms of honeybee venom used in pharmacological applications: manually (or reservoir disrupting) extracted glandular venom (GV), and venom extracted through the use of electrical stimulation (ESV).

Proteome analysis of hemolymph changes during the larval to pupal development stages of honeybee workers (Apis mellifera ligustica).

Hemolymph is vital for the flow and transportation of nutrients, ions, and hormones in the honey bee and plays role in innate immune defense. The proteome of the hemolymph changes over the life of a honey bee, but many of these changes are not well characterized, including changes during the life cycle transition from the larval to pupal stages of workers. We used two-dimensional gel electrophoresis, mass spectrometry, bioinformatics, and Western blot to analyze the proteome changes of the honeybee hemolymph during the transition from newly hatched larvae to five-day-old pupae.

Novel aspects of understanding molecular working mechanisms of salivary glands of worker honeybees (Apis mellifera) investigated by proteomics and phosphoproteomics.

Unlabelled: Honeybee salivary glands (SGs) are important exocrine glands. However, the molecular basis of how SGs fulfill their biological duty is still elusive. Proteomics and phosphoproteomics of cephalic SG (HSG) and thoracic SG (TSG) were compared between normal and single-cohort honeybee colonies.