Astragalus mongholicus Bunge and Panax notoginseng formula (A&P) improves renal mesangial cell damage in diabetic nephropathy by inhibiting the inflammatory response of infiltrated macrophages.

Background: Diabetic nephropathy (DN) is one of the main causes of end-stage renal disease with scantly effective treatment. Numerous evidences indicated that macrophages play an important role in the occurrence and pathogenesis of DN by secreting inflammatory cytokines. Mincle is mainly expressed in macrophages and promotes kidney inflammation and damage of acute kidney injury.

Effect of soil leaching on the toxicity thresholds (ECx) of Zn in soils with different properties.

Currently, the scientific basis for establishing soil environmental criteria is lacking. In order to establish reasonable soil environmental criteria values suitable for soils with different properties, this study selected soils from 16 different sites to determine the toxicity threshold of Zn based on toxicity tests of barley root elongation. In addition, leaching treatments were set up in seven soils with different properties to eliminate the influence of the accompanying anions (Cl) on the determination of the Zn toxicity threshold.

Cyclophosphamide inhibits Pax5 methylation to regulate the growth of retinoblastoma via the Notch1 pathway.

Retinoblastoma (Rb) is the most common intraocular malignant tumor in infants. Here, we investigated the function and mechanism of cyclophosphamide (CTX) in the development of Rb. Real-time quantitative polymerase chain reaction (RT-qPCR) results showed that paired box protein 5 (Pax5) expression was down-regulated in Rb tissues and cell lines.

Manganese facilitates cadmium stabilization through physicochemical dynamics and amino acid accumulation in rice rhizosphere under flood-associated low pe+pH.

Periodic flooding in paddy soils impacts redox behavior and induces variations in pe+pH levels. Manganese (Mn) is capable of reducing cadmium (Cd) uptake by rice. However, the processes involved in how Mn alters Cd mobilization under different pe+pH environments remain poorly understood.

Fe(III) reduction due to low pe+pH contributes to reducing Cd transfer within a soil-rice system.

Effect of Fe redox state caused by low soil pe+pH levels on Cd uptake by rice is unclear. Rice grown in pots of Cd-contaminated paddy soil were subjected to different irrigation regimes: flooding, intermittent flooding (Int-FL), and sustained soil moisture at 70% water holding capacity (WHC). Results showed low pe+pH (5.

Redistribution of iron oxides in aggregates induced by pe + pH variation alters Cd availability in paddy soils.

In this study, the effect of unstable pe + pH levels on the transformation of Fe oxides in different-sized soil fractions and its impact on Cd speciation were explored. Paddy soil samples collected from two locations in China were cultivated for two months under one of four pe + pH conditions: flooding + N (T1), flooding (T2), 70% water holding capacity (T3), and 70% water holding capacity + O (T4). Chemical analysis and X-ray diffraction (XRD) were used to identify the mineralogical phases and species of Fe and Cd in paddy soils.

MicroRNA‑363‑3p inhibits cell proliferation and induces apoptosis in retinoblastoma cells via the Akt/mTOR signaling pathway by targeting PIK3CA.

There is extensive evidence suggesting that microRNAs (miRs) can modulate the activity of oncogenes and tumor suppressors, and are associated with the occurrence of cancer. In the present study, the function of miR‑363‑3p in the progression of retinoblastoma (RB) was investigated. miR‑363‑3p expression in RB was decreased, and miR‑363‑3p protein levels were found to be inversely correlated with phosphatidylinositol‑4,5‑bisphosphate 3‑kinase catalytic subunit α (PIK3CA) levels.

Iron fractions responsible for the variation of Cd bioavailability in paddy soil under variable pe+pH conditions.

Iron (Fe) in soil is closely related to cadmium (Cd) uptake by rice plants, and soil pe + pH significantly influences Fe redox behavior. This study aimed to explore the influential mechanisms of varying pe + pH conditions on the transformation of iron oxides in the rhizosphere and the subsequent effect on Cd accumulation in rice plants. A two-month pot experiment was conducted to investigate the effect of soil pe + pH on the fractions of iron oxides and formation of iron plaque (IP), as well as the effect of these changes on Cd uptake by rice plants (Oryza sativa L.

Total saponins of Bolbostemma paniculatum (maxim.) Franquet exert antitumor activity against MDA-MB-231 human breast cancer cells via inhibiting PI3K/Akt/mTOR pathway.

Background: The aim of the present study was to examine the effects of the Bolbostemma paniculatum (Maxim.) Franquet (BP) active compound, BP total saponins (BPTS), on MDA-MB-231 cells, and investigate the underlying mechanism regarding BPTS-mediated attenuation of the PI3K/Akt/mTOR pathway.

Methods: The effect of BPTS on cytotoxicity, induction of apoptosis and migration on MDA-MB-231 cells at three different concentrations was investigated.

Sulfur application modifies cadmium availability and transfer in the soil-rice system under unstable pe+pH conditions.

The objective of this study was to investigate the responses of cadmium (Cd) availability and transfer in the soil-rice system to added sulfur (S) under unstable pe + pH conditions. Different water management conditions (flooding and aerobic treatments) cause changes in the soil pe + pH. We conducted a pot experiment to investigate the influence of S supply on soil Cd availability and Cd accumulation in rice plants (Oryza sativa L.

The association between human papillomavirus 16, 18 DNA load and E6 protein expression in cervical intraepithelial neoplasia and cancer.

Background: It has been reported that high HPV DNA load and elevated E6 protein expression correlate with cervical cancer, but no epidemiological study has been performed.

Objectives: To assess the association between type-specific HPV DNA load and presence of E6 protein in cervical intraepithelial neoplasia and cancer.

Study Design: This study was a cross-sectional multicenter study performed between 2013 and 2017.

Typhae pollen polysaccharides ameliorate diabetic retinal injury in a streptozotocin-induced diabetic rat model.

Ethnopharmacological Relevance: According to ancient traditional Chinese medicine, Typhae Pollen (TP) is commonly used to treat fundus haemorrhage because it improves blood circulation.

Aims Of The Study: This study evaluated the role of the main TP component, polysaccharides (TPP), on diabetic retinopathy (DR) and its possible mechanisms of inhibiting inflammation and improving blood circulation.

Materials And Methods: After successful establishment of a diabetic rat model, TPP was administered to diabetic rats for treatment, and the rats were sacrificed at 12 weeks.

Clinical evaluation of human papillomavirus 16/18 oncoprotein test for cervical cancer screening and HPV positive women triage.

HPV-16 and -18 account for about 80% of cervical cancers. We evaluated the performance of HPV-16/18 oncoprotein to predict precancer and cancer in corresponding tissue biopsy specimens. 1,008 women attending cervical cancer screening program and 638 women referred to colposcopy with biopsy-confirmed cervical intraepithelial neoplasia grade 2 or worse (CIN2+) from 4 hospitals were recruited (1,646 in total).

Protective effects of the Chinese herbal medicine prescription Zhujing pill on retina of streptozotocin-induced diabetic rats.

Objective: In this study, we investigate the protective effects of the Chinese herbal medicine prescription Zhujing pill (ZJP) on the development of diabetic retinopathy (DR) and explore the potential mechanisms.

Materials And Methods: Zhujing pill extract (ZJPE) was prepared, and the main components were identified by high-performance liquid chromatography (HPLC). Several serum and tissue (retina) parameters were measured, such as levels of superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), intercellular adhesion molecule 1 (ICAM-1), vascular endothelial growth factor (VEGF), interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), advanced glycation end products (AGEs), and high sensitivity C-reactive protein (hsCRP).

MicroRNA-486-5p suppresses TGF-β2-induced proliferation, invasion and epithelial-mesenchymal transition of lens epithelial cells by targeting Smad2.

The pathological development of lens epithelial cells (LECs) leads to posterior capsular opacification (PCO). This study was undertaken to investigate the effects of microRNA-486-5p (miR-486-5p) on TGF-β2-induced proliferation, invasion and epithelial-mesenchymal transition (EMT) in the lens epithelial cell line SRA01/04, and to explore the underlying molecular mechanisms. The expression of miR-486-5p in TGF-β2-induced SRA01/04 cells was down-regulated, and the expression of Smad2, p-Smad2 and p-Smad3 was up-regulated.

Evaluation of human papillomavirus detection by Abbott m2000 system on samples collected by FTA Elute™ Card in a Chinese HIV-1 positive population.

Background: HIV+/AIDS women have an increased risk of developing into CIN and cervical cancer compared to the general population. Limited medical resource and the lack of AIDS relevant knowledge impair the coverage and efficiency of cervical cancer screening.

Objectives: To compare the clinical performance of self-collected dry storage medium (FTA Elute card) and physician-collected PreservCyt medium in detection of high risk human papillomavirus (HR HPV) among HIV-1 positive population.

p16/Ki-67 co-expression associates high risk human papillomavirus persistence and cervical histopathology: a 3-year cohort study in China.

Purpose: To evaluate the association of p16/Ki-67 co-expression and persistence of high-risk human papillomavirus (HR-HPV) infection as well as cervical abnormalities.

Methods: We performed a 3-year cohort study among which 2498 Chinese women aged 25 to 65 years were screened by different HPV tests in 2011. 690 women who were positive at any of the tests and a random sample of 164 women with all negative results received colposcopy, cervical specimens for cobas HPV test (Roche diagnostics) were collected before colposcopy; of this group, 737 cervical specimens were collected to perform cobas, Liquid-based cytology, HPV E6 test (Arbor Vita Corporation) and p16/Ki-67 dual staining (Roche diagnostics) in 2014.

Elevated Expression of Human Papillomavirus-16/18 E6 Oncoprotein Associates with Persistence of Viral Infection: A 3-Year Prospective Study in China.

Background: An association between high-risk human papillomavirus (HR-HPV) oncoprotein expression and viral persistence has been suggested by the outcome of etiology studies, but there are no epidemiologic studies evaluating that link.

Methods: We performed a 3-year prospective study in which 2,498 Chinese women ages 25 to 65 years were screened by six screening tests, including the Onco E6: Cervical Test (Arbor Vita Corporation) in 2011 (baseline). Six-hundred and ninety women who were positive for any of the tests and a random sample of 164 women with all negative results received colposcopy, and cervical specimens for the cobas 4800 HPV test ("cobas," Roche Molecular Systems) were collected before colposcopy; of this group, 737 cervical specimens were collected to perform cobas and Onco E6: Cervical Test in 2014 (follow-up).

Evaluation of p16/Ki-67 dual staining in detection of cervical precancer and cancers: a multicenter study in China.

Purpose: To analyze the clinical performance of p16/Ki-67 dual-stained cytology identifying high-grade cervical intraepithelial neoplasia (CIN2+) in Chinese women.

Methods: 1079 women attending ongoing cervical cancer screening and 211 "enriched" women aged ≥30yrs with biopsy-confirmed CIN2+ from five Chinese hospitals were enrolled during year 2014-2015. Cervical specimens were collected for high-risk human papillomavirus (HR-HPV) DNA analysis, Liquid-based cytology (LBC) and p16/Ki-67 dual staining.

[Association between life style, diet intake and high risk-human papillomavirus persistent infection among rural women in Xinmi City, Henan Province].

Objective: To explore the association between life style, diet intake and high risk human papillomavirus (HR-HPV) persistent infection among Chinese rural women living in Xinmi City, Henan Province.

Methods: In 2010, a 3-year prospective study in which 2500 women were enrolled and screened by different HR-HPV DNA tests was conducted, part of women among them was followed and tested for HR-HPV DNA in 2012 and 2014. Furthermore, socio demographic factors, gynecological information and diet intake in the past 12 months were collected by self-designed questionnaire in 2014.

[Regulatory effects of ninjurin-1 on adhesion of myeloid cells in the retina at the early stage of diabetic rats].

Objective: To investigate the regulatory effects of ninjurin-1 on adhesion of myeloid cells in the retina at the early stage of diabetic rats.

Methods: Experimental study. The rat diabetic model was induced by intraperitoneal injection of streptozotocin.