1,25-(OH)D protects Schwann cells against advanced glycation end products-induced apoptosis through PKA-NF-κB pathway.

Aims: To explore the effect and mechanism of 1, 25-(OH)D on Schwann cell apoptosis induced by advanced glycation end products.

Main Methods: Schwann cells, isolated from rodent sciatic nerve were incubated with AGE-modified bovine serum albumin(AGE) to mimic diabetic conditions and 1,25-(OH)D was used as protector. Cell apoptosis was detected by PI/Annexin-V staining, caspase 3 activity assay and western blotting for caspase 3 and PARP.