Engineering a controllable and reversible switch for CAR-based cellular immunotherapies via a genetic code expansion system.

Background: As one of the most promising adoptive cell therapies, CAR-T cell therapy has achieved notable clinical effects in patients with hematological tumors. However, several treatment-related obstacles remain in CAR-T therapy, such as cytokine release syndrome, neurotoxicity, and high-frequency recurrence, which severely limit the long-term effects and can potentially be fatal. Therefore, strategies to increase the controllability and safety of CAR-T therapy are urgently needed.

scDCA: deciphering the dominant cell communication assembly of downstream functional events from single-cell RNA-seq data.

Cell-cell communications (CCCs) involve signaling from multiple sender cells that collectively impact downstream functional processes in receiver cells. Currently, computational methods are lacking for quantifying the contribution of pairwise combinations of cell types to specific functional processes in receiver cells (e.g.

Lactobacillus reuteri metabolites alleviate apple replant disease (ARD) by driving beneficial bacteria to reshape the core root microbiome.

Previous studies have shown that the bacterial fertilizer Lactobacillus reuteri (LBR) significantly alleviates apple replant disease (ARD), but the mechanism behind its effectiveness remains unclear. This study investigated the effects of key LBR metabolites on the rhizosphere microbial community. The biocontrol function of extracellular polysaccharides (EPS) was examined and shown to be further enhanced after optimizing the fermentation conditions.

Fault Diagnosis Method for Converter Stations Based on Fault Area Identification and Evidence Information Fusion.

DC converter stations have a high voltage level, a long transmission distance, and complex internal equipment, and contain power electronic devices, which seriously endanger the stable operation of the system itself and the active distribution network at the receiving end when faults occur. Accurate fault analysis and diagnosis are critical to the safe and stable operation of power systems. Traditional fault diagnosis methods often rely on a single source of information, leading to issues such as insufficient information utilization and incomplete diagnostic scope when applied to DC transmission systems.

Ameliorating lipopolysaccharide induced acute lung injury with Lianhua Qingke: focus on pulmonary endothelial barrier protection.

Background: Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) has long posed challenges in clinical practice, lacking established preventive and therapeutic approaches. Lianhua Qingke (LHQK), a patented traditional Chinese medicine (TCM), has been found to have anti-inflammatory effects for ameliorating ALI/ARDS induced by lipopolysaccharide (LPS). This study aimed to investigate the effects and potential mechanisms of LHQK on endothelial protection in LPS-induced ALI/ARDS and in LPS-induced human pulmonary microvascular endothelial cells (HPMECs) injury

Methods: In the animal experiment, we induced an ALI/ARDS model by intratracheal injection of LPS (5 mg/mL).

Purification Processes for Generating Cationic Lignin-Acrylamide Polymers.

Purification is an essential step in many polymerization processes for fabricating highly pure polymers. This study considered various purification methods for purifying the product of lignin, acrylamide (AM), and diallyl dimethylammonium chloride (DADMAC) copolymerization reactions at a laboratory scale. The charge density, yield, molecular weight, and solubility analyses confirmed that ethanol extraction and membrane filtration were the most effective processes for producing lignin-p(AM)-p(DADMAC).

Acceptance of Rubella-Containing Vaccine and Factors Associated with Acceptance among Women of Reproductive Age in China: A Cross-Sectional Study.

: To investigate the acceptance and factors influencing acceptance of rubella-containing vaccine (RCV) among women of reproductive age in Guangdong, Henan, Hubei, Liaoning, Shanxi, Sichuan and Zhejiang provinces of China. : Using a stratified sampling method, we selected one urban and one rural community health services center in each of two cities in seven provinces. From these centers, we recruited women 15-49 years of age to complete a questionnaire on their willingness to receive RCV and factors influencing willingness.

IGF1R signaling in perinatal mesenchymal stem cells determines definitive hematopoiesis in bone marrow.

During the transition from embryonic to adult life, the sites of hematopoiesis undergo dynamic shifts across various tissues. In adults, while bone marrow becomes the primary site for definitive hematopoiesis, the establishment of the bone marrow niche for accommodating hematopoietic stem cells (HSCs) remains incompletely understood. Here, we reveal that perinatal bone marrow mesenchymal stem cells (BMSCs) exhibit highly activated insulin-like growth factor 1 receptor (IGF1R) signaling compared to adult BMSCs.

Correlation between cognitive impairment and body composition indicators in patients with chronic kidney disease after hemodialysis.

Objective: To examine the relationship between cognitive impairment and body composition indicators in chronic kidney disease (CKD) patients post-hemodialysis.

Methods: This retrospective study included 110 CKD patients admitted to Beijing Luhe Hospital, Capital Medical University between January 2019 and January 2023. General clinical data and body composition indicators were compared between patients with and without cognitive impairment.

Impact of beta-blocker usage on delirium in patients with sepsis in ICU: a cross-sectional study.

Introduction: Delirium in patients with sepsis can be life-threatening. This study aims to investigate the impact of the use of beta-blockers on the occurrence of delirium in patients with sepsis in the ICU by utilizing a comprehensive dataset.

Methods: This is a cross-sectional study conducted using the data obtained from a single ICU in the USA.

Safety and efficacy of human amniotic epithelial stem cell eye drops in ocular chronic graft--host disease.

Not available.

New progress in HBV control and the cascade of health care for people living with HBV in China: evidence from the fourth national serological survey, 2020.

Background: Since 1992, when recombinant hepatitis B vaccine was introduced in China, government health officials have used nationally representative serological surveys to monitor progress in prevention and control of hepatitis B. In 2020, we conducted the fourth seroepidemiological survey, which for the first time included medical evaluation of the clinical status of HBsAg positive subjects over the age of 15 and their medical management. We report survey results in comparison with the three previous surveys.

GMP-compliant automated radiosynthesis of [F] SynVesT-1 for PET imaging of synaptic vesicle glycoprotein 2 A (SV2A).

Background: A novel positron emission tomography (PET) imaging tracer, [F] SynVesT-1, targeting synaptic vesicle glycoprotein 2 (SV2A), has been developed to meet clinical demand. Utilizing the Trasis AllinOne-36 (AIO) module, we've automated synthesis to Good Manufacturing Practice (GMP) standards, ensuring sterile, pyrogen-free production. The fully GMP-compliant robust synthesis of [F] SynVesT-1 boosting reliability and introducing a significant degree of simplicity and its comprehensive validation for routine human use.

Shensong yangxin, a multi-functional traditional Chinese medicine for arrhythmia: A review of components, pharmacological mechanisms, and clinical applications.

As a common cardiovascular disease (CVD), Arrhythmia refers to any abnormality in the origin, frequency, rhythm, conduction velocity, timing, pathway, sequence, or other aspect of cardiac impulses, and it is one of the common cardiovascular diseases in clinical practice. At present, various ion channel blockers are used for treatment of arrhythmia that include Na ion channel blockers, K ion channel blockers and Ca ion channel blockers. While these drugs offer benefits, they have led to a gradual increase in drug-related adverse reactions across various systems.

YAP1 regulates thrombopoiesis by binding to MYH9 in immune thrombocytopenia.

Immune thrombocytopenia (ITP) is a complicated bleeding disease characterized by a sharp platelet reduction. As a dominating element involved in ITP, megakaryocytes (MKs) are responsible for thrombopoiesis. However, the mechanism underlying the dysregulation of thrombopoiesis that occurs in ITP remains unidentified.

Von Willebrand factor exacerbates heart failure through formation of neutrophil extracellular traps.

Background And Aims: Heart failure (HF) is a leading cause of mortality worldwide and characterized by significant co-morbidities and dismal prognosis. Neutrophil extracellular traps (NETs) aggravate inflammation in various cardiovascular diseases; however, their function and mechanism of action in HF pathogenesis remain underexplored. This study aimed to investigate the involvement of a novel VWF-SLC44A2-NET axis in HF progression.

Methyl-CpG-binding 2 K271 lactylation-mediated M2 macrophage polarization inhibits atherosclerosis.

Posttranslational modifications of proteins have not been addressed in studies aimed at elucidating the cardioprotective effect of exercise in atherosclerotic cardiovascular disease (ASCVD). In this study, we reveal a novel mechanism by which exercise ameliorates atherosclerosis via lactylation. Using ApoE mice in an exercise model, proteomics analysis was used to identify exercise-induced specific lactylation of MeCP2 at lysine 271 (K271).

Tröger's Base-Embedded Pillararenes─Macrocycles with Both Fixed and Conformational Chirality.

Tröger's base-embedded pillararenes (), which combine Tröger's base () with dialkoxybenzene units, were prepared via a fragment-coupling macrocyclization strategy. The unit in macrocycle provides a fixed chiral source, while 1,4-alkoxybenzene segments flip quickly to change their arrangement, which could provide reversible conformational chirality for those macrocycles. This rare example of macrocycles holding both fixed and conformational chirality lays a good foundation for expanding pillararenes using the fixed chiral source.