Targeting protein tyrosine phosphatase non-receptor type 6 (PTPN6) as a therapeutic strategy in acute myeloid leukemia.

Acute myeloid leukemia (AML) is a heterogeneous hematologic malignancy characterized by the clonal expansion of myeloid progenitor cells. Despite advancements in treatment, the prognosis for AML patients remains poor, highlighting the need for novel therapeutic targets. Protein Tyrosine Phosphatase Non-Receptor Type 6 (PTPN6), also known as SHP-1, is a critical regulator of hematopoietic cell signaling and has been implicated in various leukemias.

Bioinformatics Analysis and Immunogenicity Assessment of the Novel Multi-Stage DNA Vaccine W541 Against Mycobacterium Tuberculosis.

Background: Vaccination is one of the effective measures to prevent latent tuberculosis infection (LTBI) from developing into active tuberculosis (TB). Applying bioinformatics methods to pre-evaluate the biological characteristics and immunogenicity of vaccines can improve the efficiency of vaccine development.

Objectives: To evaluate the immunogenicity of TB vaccine W541 and to explore the application of bioinformatics technology in TB vaccine research.

Infliximab for parenchymal neuro-Behçet's syndrome: case series and meta-analysis.

Objectives: This study aims to evaluate the efficacy and safety of infliximab (IFX) in patients with parenchymal neuro-Behçet's syndrome (p-NBS).

Methods: We retrospectively analysed eleven p-NBS patients treated with IFX at our institution and combined them with studies from database searches for a meta-analysis. Pooled estimates of clinical response (complete and partial remission) and MRI improvement at months 3, 6, and 12 were calculated.

[Phenotypic and genetic analysis of a Chinese pedigree affected with Hereditary antithrombin deficiency due to a novel variant of SERPINC1 gene].

Objective: To analyze the clinical phenotype and genetic characteristics of a Chinese pedigree affected with Hereditary antithrombin deficiency.

Methods: A pedigree diagnosed at the the Second Affiliated Hospital of Wenzhou Medical University, Yuying Children's Hospital in June, 2020 was selected as the study subject. Plasma prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen (FIB), and thrombin time (TT) of the probands and their pedigree members were determined using a STA-R automatic coagulation analyzer.

Transformation pathways of enrofloxacin chlorination disinfection by-products in constructed wetlands.

Antibiotic residues and their chlorinated disinfection by-products (Cl-DBPs) have adverse effects on organisms in aquaculture water. Taking enrofloxacin (ENR) as target antibiotic, this study investigated the degradation and transformation of ENR Cl-DBPs in constructed wetlands (CWs). Results showed that, ENR and its Cl-DBPs affected the biodegradation of CWs at the preliminary stage, but did not affect the adsorption by plant roots, substrates, and biofilms.

Degradation products and transformation pathways of sulfamethoxazole chlorination disinfection by-products in constructed wetlands.

Antibiotics and available chlorine coexist in multiple aquatic environments, and thus antibiotics and their chlorinated disinfection by-products (Cl-DBPs) have been a great concern for the nature and human health. Herein, the degradation intermediates and transformation pathways of sulfamethoxazole (SMX) Cl-DBPs in constructed wetlands (CWs) were investigated. A total of five SMX Cl-DBPs and their twenty degradation products in CWs was identified in this study.

Sulfadiazine chlorination disinfection by-products in constructed wetlands: Identification of biodegradation products and inference of transformation pathways.

Disinfection by-products (DBPs) formed from chlorination of antibiotics have greater toxicity than their parent compounds. Herein, this study investigated the biotransformation process of sulfadiazine Cl-DBPs in constructed wetlands (CWs). Results showed that, S atom on sulfonyl group, and N atoms on primary and secondary amine groups were the most reactive sites of sulfadiazine molecule.

Experimental investigation of the period-adding bifurcation route to chaos in plasma.

Since the characteristic timescales of the various transport processes inside the discharge plasma span several orders of magnitude, it can be regarded as a typical fast-slow system. Interestingly, in this work, a special kind of complex oscillatory dynamics composed of a series of large-amplitude relaxation oscillations and small-amplitude near-harmonic oscillations, namely, mixed-mode oscillations (MMOs), was observed. By using the ballast resistance as the control parameter, a period-adding bifurcation sequence of the MMOs, i.

A small molecule exerts selective antiviral activity by targeting the human cytomegalovirus nuclear egress complex.

Human cytomegalovirus (HCMV) is an important pathogen for which new antiviral drugs are needed. HCMV, like other herpesviruses, encodes a nuclear egress complex (NEC) composed of two subunits, UL50 and UL53, whose interaction is crucial for viral replication. To explore whether small molecules can exert selective antiviral activity by inhibiting NEC subunit interactions, we established a homogeneous time-resolved fluorescence (HTRF) assay of these interactions and used it to screen >200,000 compound-containing wells.

Baricitinib for the treatment of refractory vascular Behçet's disease.

Objective: The pilot study aims to evaluate the effectiveness and safety of baricitinib in Behcet's Disease (BD) patients with refractory vascular involvement.

Methods: We consecutively enrolled vascular/cardiac BD patients who received baricitinib (2 mg/day) along with glucocorticoids (GCs) and immunosuppressants in our center. Efficacy assessment mainly depends on the proportion of clinical remission and side effects were recorded.

Myelin basic protein and index for neuro-Behçet's disease.

Neuro-Behçet's disease (NBD) contributes to poor prognosis in BD patients which lacks reliable laboratory biomarkers in assessing intrathecal injury. This study aimed to determine the diagnostic value of myelin basic protein (MBP), an indicator of central nervous system (CNS) myelin damage, in NBD patients and disease controls. Paired samples of cerebrospinal fluid (CSF) and serum MBP were measured using ELISA, while IgG and Alb were routinely examined before the MBP index was developed.

Degradation and transformation of linear alkyl-benzene sulfonates (LAS) in integrated constructed wetland-microbial fuel cell systems.

Linear alkylbenzene sulfonates (LAS) are the most commonly-used anionic surfactants in cleaning agents and detergents. Taking sodium dodecyl benzene sulfonate (SDBS) as the target LAS, this study investigated the degradation and transformation of LAS in integrated constructed wetland-microbial fuel cell (CW-MFC) systems. Results showed that, SDBS was able to improve the power output and reduce the internal resistance of CW-MFCs by reducing transmembrane transfer resistance of organics and electrons because of the amphiphilicity and solubilization, however, SDBS with relatively high concentration had a great potential to inhibit electricity generation and organics biodegradation of CW-MFCs because of the toxic effects on microorganisms.

Baricitinib for the treatment of intestinal Behçet's disease: A pilot study.

Objectives: The pilot study aims to explore the efficacy and safety of baricitinib in treating refractory intestinal Behçet's disease (BD).

Methods: We consecutively enrolled patients with refractory intestinal BD from October 2020 to September 2022. They were treated with baricitinib 2-4 mg daily, with background glucocorticoids and immunosuppressants.

Numerical simulation of the bifurcation-remerging process and intermittency in an undriven direct current glow discharge.

As a complex nonlinear medium, gas discharge plasma can exhibit various nonlinear discharge behaviors. In this study, in order to investigate the chaos phenomenon in the subnormal glow region of an undriven direct current glow discharge, a two-dimensional plasma fluid model is established coupled with a circuit model as a boundary condition. Using the applied voltage as control parameter in the simulation, the complete period-doubling bifurcation and inverse period-doubling bifurcation processes in the oscillation region are found, and the influence of the applied voltage on the spatiotemporal distribution of plasma parameters during the bifurcation-remerging process is examined.

Prediction of Th1 and Cytotoxic T Lymphocyte Epitopes of and Evaluation of Their Potential in the Diagnosis of Tuberculosis in a Mouse Model and in Humans.

Latent tuberculosis infection (LTBI) is the primary source of tuberculosis (TB) but there is no suitable detection method to distinguish LTBI from active tuberculosis (ATB). In this study, five antigens of Mycobacterium tuberculosis belonging to LTBI and regions of difference (RDs) were selected to predict Th1 and cytotoxic T lymphocyte (CTL) epitopes. The immunodominant Th1 and CTL peptides were identified in mouse models, and their performance in distinguishing LTBI from ATB was determined in mice and humans.

Phenotypic and genetic analyses of four cases of coagulation factor XII deficiency.

Objectives: To identify the clinical phenotypic and molecular pathogeneses of four cases of coagulation factor XII deficiency and to deepen the cognition of this disease.

Methods: Coagulation tests were performed through one stage of coagulation on a STAGO coagulation analyser. Coagulation factor XII antigen was detected using enzyme-linked immunosorbent assay.

[Analysis of two pedigrees affected with inherited dysfibrinogenemia due to a novel c.1115 T>A variant of the FGB gene].

Objective: To analyze the phenotype and genotype of two Chinese family with inherited dysfibrinogenemia and the molecular pathogenic mechanism.

Methods: In the probands and their family members, coagulation routine, fibrinogen activity (Fg: A) and fibrinogen antigen (Fg: Ag) were detected. To find the mutation and exclude single nucleotide polymorphisms, all the exons and exons-intron boundaries of fibrinogen genes (FGA, FGB and FGG) were amplified by Ploymerase Chain Reaction (PCR), then sequenced.

The Efficacy and Safety of the Combined Therapy of Sodium-Glucose Co-Transporter-2 Inhibitors and Angiotensin Receptor-Neprilysin Inhibitor in Patients With Heart Failure With Reduced Ejection Fraction: A Meta-Analysis of the EMPEROR-Reduced and DAPA-HF Sub-Analysis.

Background: Both sodium-glucose co-transporter-2 (SGLT-2) inhibitors and angiotensin receptor-neprilysin inhibitor (ARNI) were recommended to treat heart failure with reduced ejection fraction (HFrEF). However, no trial was conducted to assess the efficacy and safety of the combined therapy of SGLT-2 inhibitors and ARNI in patients with HFrEF.

Methods: We performed a meta-analysis of the prespecified subgroups from DAPA-HF and EMPEROR-Reduced trials.

Epigenetic regulation mechanism of DNA methylation and miRNAs on the expression of the gene in patients with ischemic stroke.

5-lipoxygenase-activating protein (FLAP), encoded by the arachidonate 5-lipoxygenase-activating protein gene, can adjust the biogenesis of proinflammatory leukotrienes to increase the adhesion and permeability of the vascular internal wall. Moreover, it participates in the process of atherosclerosis and is closely associated with ischemic stroke (IS). Accumulating evidence has shown that the expression levels of the gene are upregulated in patients with IS.

Association of Preexisting Asthma and Other Allergic Diseases With Mortality in COVID-19 Patients: A Systematic Review and Meta-Analysis.

Respiratory viruses are known to contribute to asthma exacerbations. A meta-analysis of three studies reported no association between coronavirus disease 2019 (COVID-19) mortality and preexisting asthma. This study aimed to investigate the mortality of patients with COVID-19 in relation to preexisting asthma and other allergic diseases associated with changes in respiratory function.

Synergistic effects of natural ventilation and animal disturbance on oxygen transfer, pollutants removal and microbial activity in constructed wetlands.

This study investigated the feasibility of combining natural ventilation and animal disturbance in constructed wetlands (CWs) and the joint effects on oxygen transfer, microbial activity, organics, and nitrogen removal. The results showed that natural ventilation extended the habitat depth of earthworms by approximately 10 cm by significantly improving oxygen transfer in CWs; in turn, the earthworms slightly promoted the addition of oxygen inside CWs through burrowing activity. Therefore, the interaction between natural ventilation and animal disturbance in CWs mutually reinforced oxygen transfer, enzymatic activity, and the ammonification, nitrification, and aerobic degradation of organics.

[A case of inherited afibrinogenemia caused by an IVS7-12A>G splice mutation of FGG gene].

Objective: To explore the genetic basis for a Chinese pedigree affected with inherited afibrinogenemia.

Methods: For the proband and his family members, prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), Fibrin(ogen) degradation products (FDPs), D-dimer (D-D), plasminogen activity (PLG:A) and the TT mixed experiment with protamine sulfate were determined with a STAGO-R automatic coagulation analyzer. The activity and antigen of fibrinogen (Fg) in plasma were measured with the Clauss method and immunonephelometry method, respectively.