Hormone effects of eighteen bisphenol analogues and their effects on cellular homeostasis and the typical signal pathways.

Through the transfer chain of surroundings from feed to the farmed-animals and ultimately the corresponding livestock and poultry products, people are exposed to large amounts of bisphenol analogues (BPs), such as rational emissions from manufacturing plants, feed packaging bags and food packaging contact. Some BPs have been reported to show certain toxicological effects, especially, estrogen and endocrine disrupting effect. With the increasing application of BPs, the problem is becoming more and more serious.

Glucose oxidase as an alternative to antibiotic growth promoters improves the immunity function, antioxidative status, and cecal microbiota environment in white-feathered broilers.

This study aimed to demonstrate the effects of glucose oxidase (GOD) on broilers as a potential antibiotic substitute. A total of four hundred twenty 1-day-old male Cobb500 broilers were randomly assigned into five dietary treatments, each with six replicates (12 chicks per replicate). The treatments included two control groups (a basal diet and a basal diet with 50 mg/kg aureomycin) and three GOD-additive groups involving three different concentrations of GOD.

Kinetics of PCDD/Fs from feed to cow milk and its implications for food safety.

Guideline levels of polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDD/Fs) in feed and food have been separately recommended for the official food safety control around the world. However, less is considered about the transfer effect of PCDD/Fs from feed to food, and consequently possible human exposure risk. In this study, different controlled feeding experiments (E1 group: 4.

Occurrence of some legacy and emerging contaminants in feed and food and their ranking priorities for human exposure.

The "feed-to-food" pathway is one of the most important routes for human exposure to manmade contaminants. The contaminants could threaten human health through the "feed-to-food" route and have recently become of great public concern. This review selects the representative legacy and emerging contaminants (ECs), such as polybrominated diphenyl ethers (PBDEs), novel brominated flame retardants (NBFRs), organophosphate esters (OPEs), short-chain chlorinated paraffins (SCCPs), and per- and polyfluoroalkyl substances (PFASs), regarding their occurrence in feed and food, as well as their metabolites and transport in farming and livestock ecosystems.

Development of an Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Method for Hydroxylated Polychlorinated Biphenyls in Animal-Derived Food.

Hydroxylated polychlorinated biphenyls (OH-PCBs) are a group of metabolites biotransformed from polychlorinated biphenyls by animals with higher toxicities than their parent compounds. The present work developed and validated an analytical method for determinating penta-, hexa-, and hepta-chlorine substituted OH-PCBs in animal-derived food based on ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) with isotope-dilution. The target analytes were extracted with a 50% n-hexane/dichloromethane (/), purified by sulfuric acid-silica gel, and separated by 5% hydrated silica gel, achieving a final concentration of 100 times before injection to LC-MS/MS.

New Insights into the Deposition of Zearalenone in Minipigs: A Suitable Bioindicator for Internal Exposure.

The detrimental footprint of mycotoxins in agriculture and on animal production has been widely recognized, especially in swine. Despite an increased number of research evaluating the toxicokinetics of mycotoxins in animal organisms, the absorption, distribution, metabolization, and excretion (ADME) patterns of zearalenone (ZEN) need further understanding. Furthermore, bioindicator for ZEN exposure in individual pigs has yet to be characterized.

Fluorene-9-bisphenol regulates steroidogenic hormone synthesis in H295R cells through the AC/cAMP/PKA signaling pathway.

Fluorene-9-bisphenol (BHPF), which has been used as a substitute for bisphenol A (BPA) in consumer goods and industrial products, can be detected in environmental media and human urine. BHPF has been reported to have endocrine-disrupting effects, whereas deleterious effects on steroidogenesis in H295R cells and underlying mechanisms are still unclear. Here, we investigated effects of BHPF on steroidogenesis using human adrenocortical carcinoma cells (H295R).

Rapid and reagent-free bioassay using autobioluminescent yeasts to detect agonistic and antagonistic activities of bisphenols against rat androgen receptor and progesterone receptor.

Bisphenol A (BPA) and its analogues have been classified as endocrine disruptors via binding to nuclear receptors. Two novel bioassays, BLYrARS and BLYrPRS, were developed for rapid detection of agonistic and antagonistic activities of BPA and five of its analogues binding rat androgen receptor (rAR) and rat progesterone receptor (rPR). The reporter bioassay was based on two autonomously bioluminescent strains of the yeast Saccharomyces cerevisiae, recombined with a bacterial luciferase reporter gene cassette (lux) that can produce autofluorescence, regulated by the corresponding hormone response element acting as the responsive promoter.

Comparative In Vitro and In Vivo Hydroxylation Metabolization of Polychlorinated Biphenyl 101 in Laying Hens: A Pilot Study.

Polychlorinated biphenyls (PCBs) can be metabolized into hydroxylated PCBs (OH-PCBs) that exhibit greater toxicity than their parent compounds. In particular, 2,2',4,5,5'-pentachlorobiphenyl (PCB 101) is commonly found in chicken feeds and breeding environments, although information on the biotransformation of this PCB in chickens is lacking. In this study, the hydroxylation metabolization of PCB 101 was assessed based on in vitro trials with Sanhuang chicken liver microsomes and in vivo experiments with Hy-Line Brown hens.

Occurrence of per- and polyfluoroalkyl substances (PFASs) in raw milk and feed from nine Chinese provinces and human exposure risk assessment.

The per- and polyfluoroalkyl substances (PFASs) are substantially produced and applied in industrial and domestic products, which have recently aroused great public concern for their potential toxicity to humans. In the present study, raw milk (n = 107) and cow feed samples (n = 70) were collected across nine Chinese provinces, in order to investigate the occurrence of PFASs in milk and feed, and the human exposure risk to milk. The concentrations of PFASs are in the range of < method detection limit -9.

Metal-Organic Frameworks-Based Sensors for Food Safety.

Food contains a variety of poisonous and harmful substances that have an impact on human health. Therefore, food safety is a worldwide public concern. Food detection approaches must ensure the safety of food at every step of the food supply chain by monitoring and evaluating all hazards from every single step of food production.

Study of eighteen typical bisphenol analogues as agonist or antagonist for androgen and glucocorticoid at sub-micromolar concentrations in vitro.

Bisphenol A and its substitutions are commonly used to manufacture epoxy resins, plastic materials and different kinds of daily necessities. In this process, a large number of bisphenol analogues (BPs) are continuously released directly/indirectly into the environment. Through the chain of environment-feed-farmed-animals-livestock and poultry products, BPs present the low concentration but chronic exposure for surroundings and environment.

Enzymatic probe sonication for quick extraction of total bisphenols from animal-derived foods: Applicability to occurrence and exposure assessment.

A high demand exists in bisphenols (BPs) screening studies for quick, reliable and straightforward analytical methods that generate data faster and simultaneously. Herein, we describe a combination of enzymatic probe sonication (EPS) and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for quick extraction and simultaneous quantification of eight important BPs in animal-derived foods. Results obtained demonstrated that the ultrasonic probe power could not only enhance the enzymatic hydrolysis efficiency, but also accelerate the liquid-liquid extraction procedure.

[Simultaneous determination of 37 mycotoxins in grain and animal feed by impurity adsorption purification coupled with ultra-performance liquid chromatography-tandem mass spectrometry].

A rapid method based on ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed for the simultaneous determination of 37 mycotoxins having various physicochemical properties in grain and animal feed samples. The 37 analytes were extracted from ground samples with acetonitrile-water-formic acid (84:15.9:0.

Development and validation of an ultra performance liquid chromatography-tandem mass spectrometry method for twelve bisphenol compounds in animal feed.

Bisphenol compounds (BPs) are a group of environmental contaminants with endocrine-disrupting effects both for humans and animals. The present work developed a sensitive analytical method for the detection of multiple BPs in the animal feed based on ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) with post-column ammonium hydroxide (NHOH) infusion. A modified QuEChERS method was incorporated into the extraction and purification processes.

Simultaneous adsorption and determination of bisphenol compounds in water medium with a Zr(IV)-based metal-organic framework.

A chemically stable Zr(IV)-based metal-organic framework (BUT-17) has been explored for simultaneous adsorption and determination of bisphenol compounds (BPs) in aqueous medium. The prepared BUT-17 possesses a large surface area (2936 m g) and excellent fluorescent performance. An adsorption capacity of 111 mg g for bisphenol A (BPA) with a rapid adsorption rate (1.

Be Aware of Organophosphate Diesters as Direct Sources in Addition to Organophosphate Ester Metabolites in Food Supplies.

The substantial application of organophosphate triesters (tri-OPEs) may lead to a concentration escalation of their major metabolites, organophosphate diesters (di-OPEs) in animal-derived and plant-derived animal protein supplement feeds (APFs). APFs are major food for raised animals and may bring OPEs into the food supply. In the present study, the concentrations of Σdi-OPEs in animal-derived and plant-derived APFs were in the range of 1.

Deposition, depletion, and potential bioaccumulation of bisphenol F in eggs of laying hens after consumption of contaminated feed.

Increasing concerns over bisphenol A (BPA) as an endocrine disrupting chemical (EDC) and its adverse effects on both humans and animals have led to the substitution by structural analogs, such as bisphenol F (BPF), in many application areas. Information regarding to the carry-over of this emerging chemical in farm animals is essential for legislation and risk assessment purposes. In this study, a large-scale number of animal experiments were designed to investigate the transfer of BPF from feed to eggs.

The occurrence of bisphenol compounds in animal feed plastic packaging and migration into feed.

Animal-derived food plays an important role in human exposure to bisphenol compounds (BPs), potentially as a result of the presence of BPs in animal feed. Even so, there have been few studies regarding the source of BPs in animal feed. The objective of the present study was to assess both the occurrence of BPs in animal feed packaging and the migration of BPs from feed packaging into animal feed.

Application of gas chromatography coupled to triple quadrupole mass spectrometry (GC-(APCI)MS/MS) in determination of PCBs (mono-to deca-) and PCDD/Fs in Chinese mitten crab food webs.

Polychlorinated biphenyls (PCBs) and dibenzo-p-dioxins and furans (PCDD/Fs) are notorious persistent organic pollutants (POPs), which may bioaccumulate through food chain and play detrimental effects to organisms even at trace levels. Quantification of PCBs and PCDD/Fs in biotic samples is a great challenge. In the present study, gas chromatography coupled to triple quadrupole mass spectrometry with an atmospheric pressure chemical ionization source (GC-(APCI)MS/MS) was studied for the isotope-dilution analysis of PCBs (mono-to deca-) and PCDD/Fs in Chinese mitten crab food webs.

Organophosphate Diesters (Di-OPEs) Play a Critical Role in Understanding Global Organophosphate Esters (OPEs) in Fishmeal.

Organophosphate triesters (tri-OPEs) have recently been widely identified in aquatic ecosystems, but information on their organophosphate diester (di-OPE) metabolites is sparsely available. Herein, uniform fishmeal products were collected across the globe (the U.S.

Animal-Derived and Plant-Derived Protein Supplement Feeds Are Important Sources of Organophosphate Esters in the Food Supply.

Animal protein supplement feeds (APFs) are important raw feed materials for livestock. APFs might be susceptible to organophosphate esters (OPEs) but have not been paid attention yet. In the present study, animal-derived (meat meal, feather meal, and blood meal) and plant-derived APFs were all found to contain detectable levels of OPEs, with 16 target OPEs ranging from 12.

Optimal dietary copper requirements and relative bioavailability for weanling pigs fed either copper proteinate or tribasic copper chloride.

Background: The objective of this study was to determine the effects of supplementing Cu on growth performance, Cu metabolism and Cu-related enzyme activities of weanling pigs fed diets with two different Cu sources, and to estimate optimal Cu requirements and relative bioavailability from these two sources for pigs.

Methods: Weanling pigs were allocated to 14 treatments arranged factorially, including 6 added Cu levels (5, 10, 20, 40, 80, 160 mg/kg), and 2 mineral sources (tribasic Cu chloride, TBCC and copper proteinate, CuPro), as well as one negative control (0 mg/kg added Cu level) and one maximum allowed level treatment (200 mg/kg TBCC) for the entire 38-d experiment. Growth performance, mineral status and enzyme activities were measured at the end of this study.

Trace analysis of bisphenol A and its analogues in eggs by ultra-performance liquid chromatography-tandem mass spectrometry.

Presence of bisphenol A (BPA) and its analogues in various environmental matrices have been well studied by now, but there is still an insufficient data on the occurrence of BPA analogues in animal-derived foodstuffs. Hence, this work describes a quick, sensitive and reliable analytical method for the simultaneous determination of eight BPA analogues in eggs, applying a solid-phase extraction procedure with PRiME HLB cartridges that combines isolation of the analytes from matrix and the sample clean-up on a single column. The limits of detection (LODs) and quantification (LOQs) for BPA analogues were in the ranges of 0.