Single-cell atlas comparison across vertebrates reveals auditory cell evolution and mechanisms for hair cell regeneration.

Mammals suffer permanent hearing impairment from the loss of auditory hair cells due to their inability to regenerate. In contrast, lower vertebrates exhibit extraordinary capacity for hair cell regeneration and hearing restoration, but the mechanisms remain unclear. Here we characterize the single-cell atlas of Xenopus laevis inner ear and perform a comprehensive comparison with mouse model.

Pyrolysis behavior of sewage sludge coexisted with microplastics: Kinetics, mechanism, and product characteristics.

Microplastics can accumulate in the excess sludge from wastewater treatment plants through domestic wastewater. This study investigated the co-pyrolysis behavior of sewage sludge coexisting with two types of microplastics (polyethylene (PE) and polylactic acid (PLA)) and found a superior comprehensive pyrolysis performance. By calculating the difference between theoretical and experimental weight loss during the pyrolysis process, it was found that the incorporation of microplastics PE and PLA created a synergistic effect at 270°C-450 °C, which was confirmed through the Malek method analysis from a pyrolysis mechanism perspective that it could increase the random nuclei on each particle, that is, enhance the heterogeneous diffusion of volatiles.

Tracking Existing Factors Directly Affecting the Reproduction of Bumblebees: Current Knowledge.

Bumblebees are primary social insects and a vital class of pollinating insects. Their distinctive reproductive mode is characterized by the independent initiation and construction of the nest by the queen and the subsequent production of sufficient workers, males, and gynes following colony development. After successful mating, the queen transitions to the first phase of its annual life cycle.

Forecasting carbon dioxide emissions in Chongming: a novel hybrid forecasting model coupling gray correlation analysis and deep learning method.

Predicting regional carbon dioxide (CO2) emissions is essential for advancing toward global carbon neutrality. This study introduces a novel CO2 emissions prediction model tailored to the unique environmental, economic, and energy consumption of Shanghai Chongming. Utilizing an innovative hybrid approach, the study first applies grey relational analysis to evaluate the influence of economic activity, natural conditions, and energy consumption on CO2 emissions.

Tree-Inspired Structurally Graded Aerogel with Synergistic Water, Salt, and Thermal Transport for High-Salinity Solar-Powered Evaporation.

Solar-powered interfacial evaporation is an energy-efficient solution for water scarcity. It requires solar absorbers to facilitate upward water transport and limit the heat to the surface for efficient evaporation. Furthermore, downward salt ion transport is also desired to prevent salt accumulation.

Can the digital economy empower urban energy resource transition? A natural gas perspective.

The diffusion effect of the digital economy (DEN) has become increasingly prominent, but few scholars have investigated the energy transition effect of China's DEN. To this end, this study takes 207 cities in China as the research object to explore the potential role of DEN development in boosting energy resource transition (ERT). The endogeneity and asymmetry between variables are also analyzed.

Integrative metabolomic and transcriptomic analyses reveals the accumulation patterns of key metabolites associated with flavonoids and terpenoids of Gynostemma pentaphyllum (Thunb.) Makino.

Gynostemma pentaphyllum (Thunb.) Makino (G. pentaphyllum) is a medicinal and edible plant with multiple functions of liver protection, anti-tumor, anti-inflammation, balancing blood sugar and blood lipids.

Identification and validation of regulatory T cell-associated gene signatures to predict colon adenocarcinoma prognosis.

Colon adenocarcinoma (COAD) is a common cause of cancer-related death. Due to the difficulty in early diagnosis and drug resistance, conventional treatments are difficult to be effective. Some studies have found that the functional recovery of T cells in the tumor microenvironment, especially regulatory T cells (Tregs), plays an important role in the progression of cancer.

Microbial synthesis of antimony sulfide to prepare catechol and hydroquinone electrochemical sensor by pyrolysis and carbonization.

The application of antimony sulfide sensors, characterized by their exceptional stability and selectivity, is of emerging interest in detection research, and the integration of graphitized carbon materials is expected to further enhance their electrochemical performance. This study represents a pioneering effort in the synthesis of carbon-doped antimony sulfide materials through the pyrolysis of the mixture of microorganisms and their synthetic antimony sulfide. The prepared materials are subsequently applied to electrochemical sensors for monitoring the highly toxic compounds catechol (CC) and hydroquinone (HQ) in the environment.

Towards zero excess sludge discharge with built-in ozonation for wastewater biological treatment.

In this study, a biological treatment process, which used a built-in ozonation bypass to achieve sludge reduction, was built to treat the industrial antifreeze production wastewater (mainly composed of ethylene glycol). The results indicated there is a positive correlation between ozone dosage and sludge reduction. At the laboratory level, the MLSS in the system can be stably controlled at around 3400 mg MLSS L under the dosage of 0.

Establishment, optimization and validation of a fluorescence polarization-based high-throughput screening assay targeting cathepsin L inhibitors.

Cathepsin L (CTSL), a lysosomal cysteine proteinase, is primarily dedicated to the metabolic turnover of intracellular proteins. It is involved in various physiological processes and contributes to pathological conditions such as viral infection, tumor invasion and metastasis, inflammatory status, atherosclerosis, renal disease, diabetes, bone diseases, and other ailments. The coronavirus disease 2019 (COVID-19), with its rapid global spread and significant mortality, has been a worldwide epidemic since the late 2019s.

Engineering and transcriptome study of Saccharomyces cerevisiae to produce ginsenoside compound K by glycerol.

Synthetic biology-based engineering of Saccharomyces cerevisiae to produce terpenoid natural products is an effective strategy for their industrial application. Previously, we observed that glycerol addition was beneficial for ginsenoside compound K (CK) production in a S. cerevisiae when it was fermented using the YPD medium.

Pan-genome analysis highlights the role of structural variation in the evolution and environmental adaptation of Asian honeybees.

The Asian honeybee, Apis cerana, is an ecologically and economically important pollinator. Mapping its genetic variation is key to understanding population-level health, histories and potential capacities to respond to environmental changes. However, most efforts to date were focused on single nucleotide polymorphisms (SNPs) based on a single reference genome, thereby ignoring larger scale genomic variation.

Characterization and designing of an SAM riboswitch to establish a high-throughput screening platform for SAM overproduction in Saccharomyces cerevisiae.

S-adenosyl- l-methionine (SAM) is a high-value compound widely used in the treatment of various diseases. SAM can be produced through fermentation, but further enhancing the microbial production of SAM requires novel high-throughput screening methods for rapid detection and screening of mutant libraries. In this work, an SAM-OFF riboswitch capable of responding to the SAM concentration was obtained and a high-throughput platform for screening SAM overproducers was established.

A chromosome-level reference genome assembly of the Reeve's moray eel (Gymnothorax reevesii).

Due to potentially hostile behaviors and elusive habitats, moray eels (Muraenidae) as one group of apex predators in coral reefs all across the globe have not been well investigated. Here, we constructed a chromosome-level genome assembly for the representative Reeve's moray eel (Gymnothorax reevesii). This haplotype genome assembly is 2.

Exosomal circCOL1A2 from cancer cells accelerates colorectal cancer progression via regulating miR-665/LASP1 signal axis.

Circular RNAs (circRNAs) have been demonstrated to exert pivotal functions in cancer progression but are poorly understood in colorectal cancer (CRC). This work intends to investigate the effect and mechanism of a novel cirRNA (circCOL1A2) in CRC. Exosomes were identified via transmission electron microscope (TEM) and nanoparticle tracking analysis (NTA).

[Genetic analysis and reproductive intervention of 7 families with gonadal mosaicism for Duchenne muscular dystrophy].

Objective: To explore the genetic basis for 7 families with gonadal mosaicism for Duchenne muscular dystrophy (DMD).

Methods: For the 7 families presented at the CITIC Xiangya Reproductive and Genetic Hospital from September 2014 to March 2022, clinical data were collected. Preimplantation genetic testing for monogenic disorders (PGT-M) was carried out for the mother of the proband from family 6.

Improving CO photoconversion with ionic liquid and Co single atoms.

Photocatalytic CO conversion promises an ideal route to store solar energy into chemical bonds. However, sluggish electron kinetics and unfavorable product selectivity remain unresolved challenges. Here, an ionic liquid, 1-ethyl-3-methylimidazolium tetrafluoroborate, and borate-anchored Co single atoms were separately loaded on ultrathin g-CN nanosheets.

A DMD case caused by X chromosome rearrangement.

Duchenne/Becker muscular dystrophy (DMD/BMD) is one of the most common progressive muscular dystrophy diseases with X-linked recessive inheritance. It is mainly caused by the deletion, duplication and point mutation of gene. In rare cases, it is also caused by the destruction of gene by chromosomal structural rearrangement.

Expression of EPO and related factors in the liver and kidney of plain and Tibetan sheep.

Erythropoietin (EPO), hypoxia-inducible factor-1α (HIF-1α), hypoxia-inducible factor-2α (HIF-2α), and vascular endothelial growth factor (VEGF) are key factors in the regulation of hypoxia, and can transcriptionally activate multiple genes under hypoxic conditions, thereby initiating large hypoxic stress in the network. The liver and kidneys are important metabolic organs of the body. We assessed the expression of EPO, HIF-1α, HIF-2α, and VEGF in liver and kidney tissues of plain and Tibetan sheep using hematoxylin and eosin staining, immunohistochemistry, and RT-qPCR.

Hypoxia induced β-catenin lactylation promotes the cell proliferation and stemness of colorectal cancer through the wnt signaling pathway.

Colorectal cancer (CRC) is a common malignant tumor of digestive system. Its incidence rate and mortality rate ranks the third among all the malignant tumors. The objective of this study was to explore the role of β-catenin in the CRC progression.

Current Knowledge on Bee Innate Immunity Based on Genomics and Transcriptomics.

As important pollinators, bees play a critical role in maintaining the balance of the ecosystem and improving the yield and quality of crops. However, in recent years, the bee population has significantly declined due to various pathogens and environmental stressors including viruses, bacteria, parasites, and increased pesticide application. The above threats trigger or suppress the innate immunity of bees, their only immune defense system, which is essential to maintaining individual health and that of the colony.