Disrupting EDEM3-induced M2-like macrophage trafficking by glucose restriction overcomes resistance to PD-1/PD-L1 blockade.

Background: Immunotherapy is beneficial for some colorectal cancer (CRC) patients, but immunosuppressive networks limit its effectiveness. Cancer-associatedfibroblasts (CAFs) are significant in immune escape and resistance toimmunotherapy, emphasizing the urgent need for new treatment strategies.

Methods: Flow cytometric, Western blotting, proteomics analysis, analysis of public database data, genetically modified cell line models, T cell coculture, crystal violetstaining, ELISA, metabonomic and clinical tumour samples were conducted to assess the role of EDEM3 in immune escape and itsmolecular mechanisms.

Melatonin improves endometrial receptivity and embryo implantation via MT2/PI3K/LIF signaling pathway in sows.

Background: Increased backfat thickness of sows in early gestation is negative to reproductive performance. Endometrial receptivity is an important determinant of reproductive success, but it is unclear whether the effect of sow backfat thickness on litter size is associated with endometrial receptivity and whether melatonin treatment may have benefits. The present study seeks to answer these questions through in vitro and in vivo investigations.

Effectiveness, pharmacokinetics, and safety of triptorelin acetate microspheres in patients with locally advanced and metastatic prostate cancer.

Background: A newly generic microspheres, sustained-release formulation of triptorelin acetate 3.75 mg has been developed.

Objectives: To evaluate the efficacy, pharmacokinetics, and safety of triptorelin 1-month formulation in Chinese patients with prostate cancer.

YY1 drives PARP1 expression essential for PARylation of NONO in mRNA maturation during neuroblastoma progression.

Background: Neuroblastoma (NB), the most prevalent solid tumor in children, arises from sympathetic nervous system and accounts for 15% of pediatric cancer mortality. This malignancy exhibits substantial genetic and clinical heterogeneity, thus complicating treatment strategies. Poly(ADP-ribose) polymerase 1 (PARP1), a key enzyme catalyzing polyADP-ribosylation (PARylation), plays critical roles in various cellular processes, and contributes to tumorigenesis and aggressiveness.

Weakly supervised learning in thymoma histopathology classification: an interpretable approach.

Introduction: Thymoma classification is challenging due to its diverse morphology. Accurate classification is crucial for diagnosis, but current methods often struggle with complex tumor subtypes. This study presents an AI-assisted diagnostic model that combines weakly supervised learning with a divide-and-conquer multi-instance learning (MIL) approach to improve classification accuracy and interpretability.

Elevating the TMI threshold by incorporating QCW pumping in a high-power CW fiber oscillator.

Transverse mode instability (TMI) significantly limits the power scaling of ytterbium-doped fiber lasers. In this Letter, what we believe to be a novel TMI mitigation strategy is proposed and demonstrated in a bidirectional output fiber laser. On the basis of the continuous wave (CW) pump, integrating a quasi-continuous wave (QCW) pump can effectively improve the TMI threshold of the system.

Identification of SMAD4-mutated pancreatic ductal adenocarcinoma using preoperative contrast-enhanced MRI and clinical characteristics.

Aim: To assess the value of preoperatively contrast-enhanced MRI and clinical characteristics for identification of SMAD4-mutated pancreatic ductal adenocarcinoma (PDAC) patients.

Materials And Methods: This retrospective study included patients with surgically confirmed PDAC from January 2016 to December 2022. Based on immunostaining results indicating the mutation of SMAD4, the enrolled participants were grouped into SMAD4-mutated PDAC and non-SMAD4-mutated PDAC.

Enhanced egg white powder through liquid-state ultrasound-heating pretreatment: Properties and digestibility.

Egg white protein's functional properties and digestibility can be significantly affected by processing methods. In this study, a novel combined ultrasound-heating (UH) pretreatment protocol was developed, where liquid egg white was subjected to ultrasonic treatment (0.4 W/mL, 10 min) followed by mild heating (72 °C, 10 min) prior to drying processes.

Machine learning-based prognostic model of lactylation-related genes for predicting prognosis and immune infiltration in patients with lung adenocarcinoma.

Background: Histone lactylation is a novel epigenetic modification that is involved in a variety of critical biological regulations. However, the role of lactylation-related genes in lung adenocarcinoma has yet to be investigated.

Methods: RNA-seq data and clinical information of LUAD were downloaded from TCGA and GEO datasets.

-deficient MDBK cell based on CRISPR/Cas9 technology for enhancing IBRV replication.

(IBR), characterized by acute respiratory lesions in cattle, is a major infectious disease caused by (BoAHV-1). Control of this disease is primarily depending on vaccination. Madin-Darby bovine kidney cells (MDBK) being the main host cells and the important production platform for IBR vaccines.

Energy Management Strategy for Fuel Cell Vehicles Based on Online Driving Condition Recognition Using Dual-Model Predictive Control.

Given the urgent challenges posed by global climate change and the ongoing energy crisis, fuel cell electric vehicles (FCEVs) have emerged as a promising solution. Incorporating sophisticated energy management strategies (EMSs) into FCEVs can significantly enhance the efficiency of the complex powertrain under diverse driving conditions. In this paper, a dual-model predictive control energy management strategy based on long short-term memory (LSTM)-based driving condition recognition is proposed to enhance the economic performance of FCEVs and robustness across diverse driving conditions.

The effect of sports participation on the social identity of Chinese university students.

Objective: Strengthening youth sports and comprehensively promoting the participation of Chinese university students in sports to enhance their social acceptance are key initiatives for delivering high-quality talent for China's economic and social development.

Methods: Using the China Comprehensive Social Survey as the data source and considering social mentality and social identity, we constructed corresponding statistical models to explore how sports participation influences the social identity (class identity, economic status, and emotional belonging) of Chinese university students.

Results: Regarded from a holistic perspective, sports participation can enhance the social identity of college students in all its aspects, and the benefits of sports participation are positively related to its frequency.

A preoperative nomogram with MR elastography in identifying cytokeratin 19 status of hepatocellular carcinoma.

Objectives: Developing a nomogram integrating MR elastography (MRE)-based tumor stiffness and contrast-enhanced MRI in identifying cytokeratin 19 (CK19) status of hepatocellular carcinoma (HCC) preoperatively.

Methods: 120 CK19-negative HCC and 39 CK19-positive HCC patients undergoing curative resection were prospectively evaluated. All received MRE and contrast-enhanced MRI.

Augmenter of Liver Regeneration (ALR) Crotonylation Assists in Mitochondria-ER Contact to Alleviate Hepatic Steatosis.

Background & Aims: Crotonylation (Kcr), a newly identified post-translation modification (PTM), has been confirmed to be involved in diverse biological processes and human diseases as well. Metabolic dysfunction-associated steatotic liver disease (MASLD) poses a serious threat to people's health. Augmenter of liver regeneration (ALR) is an important liver regulatory protein, and the insufficiency of ALR expression is reported to accelerate liver steatosis progression to liver fibrosis or even hepatic carcinoma (HCC).

Effects of dietary rumen-protected glucose level and taurine supplementation on weight change and oxidative stress state of yaks after transport.

Rumen-protected glucose (RPG) and taurine (TAU) are crucial for the nutrition and physiology of ruminants, enhancing production performance and mitigating negative energy balance. This study aimed to assess the impact of RPG levels and TAU supplementation on the body weight, antioxidant capacity, immune function and stress responses in yaks before and after transport. Thirty-two healthy male yaks, aged 3 years and weighing 172.

HIV-1 viral decay in blood and semen in antiretroviral-naïve adults initiating dolutegravir/lamivudine vs. bictegravir/emtricitabine/tenofovir alafenamide.

Background: Co-formulated dolutegravir and lamivudine (DTG/3TC) is recommended as the first-line antiretroviral therapy (ART); however, the data on the viral decay in seminal plasma (SP) and blood plasma (BP), as well as changes in inflammatory biomarkers in BP, remain limited among antiretroviral-naïve people with HIV (PWH) receiving DTG/3TC. A prospective observational cohort study was conducted to compare the impact of DTG/3TC vs. bictegravir/emtricitabine/tenofovir alafenamide (BIC/FTC/TAF) on viral decay kinetics and changes in inflammatory biomarkers in antiretroviral-naïve PWH.

An Improved Reduced-Dimension Robust Capon Beamforming Method Using Krylov Subspace Techniques.

A reduced-dimension robust Capon beamforming method using Krylov subspace techniques (RDRCB) is a diagonal loading algorithm with low complexity, fast convergence and strong anti-interference ability. The diagonal loading level of RDRCB is known to become invalid if the initial value of the Newton iteration method is incorrect and the Hessel matrix is non-positive definite. To improve the robustness of RDRCB, an improved RDRCB (IRDRCB) was proposed in this study.

Relationship between intracranial pressure and neurocognitive function among older adults after radical resection of rectal cancer.

Background: Older patients are prone to postoperative cognitive decline after laparoscopic rectal cancer surgery, which may be associated with increased intraoperative intracranial pressure (ICP). This study investigated the correlation between intraoperative ICP changes, as indicated by measurements of the optic nerve sheath diameter (ONSD) using ultrasonography, and subsequent cognitive function to provide better patient care.

Aim: To evaluate changes in ICP and associated postoperative neurocognition in older adults after laparoscopic radical resection for rectal cancer.

Polymer Networks Assembled by Ruthenium Catalysts for Enhanced Water Splitting Performance in Calixarene Dye-Sensitized Photoelectrochemical Cells.

Metal-free photosensitizers for the construction of low-cost and eco-friendly dye-sensitized photoelectrochemical cells (DSPECs) have recently been greatly improved, but the optimization of water oxidation catalysts (WOCs) used in DSPECs based on metal-free dyes has received little attention. Herein, a series of polymer networks (RuTPA, RuCz, RuPr and RuTz) assembled by ruthenium WOCs (RuCHO) with various organic donors are constructed and combined with calixarene dyes to prepare DSPEC devices. The FTO|TiO|C4BTP+RuTPA photoanode shows the best performance with 85 % Faraday efficiency for oxygen production and 477 μA cm photocurrent density after 200 s chopping irradiation at 0 V vs.

Power optimization of high-power random Raman laser with a full-open cavity.

Since the concept of distributed feedback fiber random laser was put forward, random Raman fiber laser (RRFL) has made great progress in high power operation. For RRFL with a full-open cavity, the simplest cavity structure, further power scaling was restricted by the rapid increase of high-order Stokes wave. In this paper, we demonstrate that the output power of the RRFL can be further improved by optimizing the fiber length.

Comparative gut microbiome research through the lens of ecology: theoretical considerations and best practices.

Comparative approaches in animal gut microbiome research have revealed patterns of phylosymbiosis, dietary and physiological convergences, and environment-host interactions. However, most large-scale comparative studies, especially those that are highly cited, have focused on mammals, and efforts to integrate comparative approaches with existing ecological frameworks are lacking. While mammals serve as useful model organisms, developing generalised principles of how animal gut microbiomes are shaped and how these microbiomes interact bidirectionally with host ecology and evolution requires a more complete sampling of the animal kingdom.

A causal association between chemokines and the risk of lung cancer: a univariate and multivariate mendelian randomization study.

Background: Observational studies and experimental evidence have shown that chemokines play important roles in lung cancer development, progression, and treatment. However, few studies have examined the causal association between them.

Methods: Summary data of chemokines and lung cancer were obtained from genome-wide association studies.

Spatial and Single Cell Analyses Reveal Heterogeneity of DNAM-1 Receptor-Ligand Interactions that Instructs Intratumoral γδT-Cell Activity.

The dynamic interplay between tumor cells and γδT cells within the tumor microenvironment (TME) significantly influences disease progression and immunotherapy outcome. Here, we delved into the modulation of γδT-cell activation by tumor cell ligands CD112 and CD155, which interact with the activating receptor DNAM-1 on γδT cells. Spatial and single cell RNA sequencing (scRNA-seq), as well as spatial metabolome analysis, from neuroblastoma (NB) revealed that the expression levels and localization of CD112 and CD155 varied across and within tumors, correlating with differentiation status, metabolic pathways, and ultimately disease prognosis and patient survival.

Characterizing transcripts of HIV-1 different substrains using direct RNA sequencing.

Post-transcriptional processing and modification of viral RNA, including alternative splicing, polyadenylation, and methylation, play crucial roles in regulating viral gene expression, enhancing genomic stability, and increasing replication efficiency. These processes have significant implications for viral biology and antiviral therapies. In this study, using Oxford Nanopore Technology (ONT) direct RNA sequencing (DRS), we provided a comprehensive analysis of the transcriptome and epitranscriptome features of the HIV-1 B (NL4-3) subtype strain and, for the first time, characterized these features in the CRF01_AE (GX2005002) subtype strain.