Effect of collagen-based scaffolds with hydroxyapatite on the repair of cartilage defects in the rabbit knee joint.

Background: The repair of articular cartilage defects is always a significant clinical challenge in joint treatment. Therefore, the aim of this study was to investigate that the ColII-HA-CS-HAP scaffolds with BMSCs could repair cartilage defects of knee.

Methods: Bone marrow mesenchymal stem cells (BMSCs) were extracted from rabbits, identified using immunofluorescence staining, and successfully induced into chondrocytes.

Three-dimensional polarimetric ptychography.

A three-dimensional polarimetric ptychography (3D-PP) technique is proposed to reconstruct the residual stress distribution in optical elements in three dimensions by combining polarization imaging and a 3D ptychographic iterative engine (3D-PIE). 3D-PP mainly uses two orthogonal linear polarizers placed along the light direction to capture the photoelastic information within an optical element. Based on phase shift theory, a three-step phase shift method is proposed to record three dark field images, allowing for calculating the isometric line of residual stress within the optical element.

Analysis on heat transfer performance of coaxial borehole heat exchanger in a layered subsurface with groundwater.

In the realm of ground source heat pump (GSHP) installations, the operational efficiency of borehole heat exchangers (BHEs) is heavily dependent on the complex configurations of geological formations, including soil stratification and the movement of underground water. Our research investigated the influences of ground structure characteristics on the heat transfer performance of coaxial BHEs. A coaxial borehole heat exchanger with a three-dimensional design was constructed, setting a typical geology from the Xiong'an New Region as the boundary condition.

JiaGaSongTang improves chronic cholestasis via enhancing FXR-mediated bile acid metabolism.

Background: Bile acid (BA) enterohepatic circulation disorders are a main feature of chronic cholestatic diseases. Promoting BA metabolism is thus a potential method of improving enterohepatic circulation disorders, and treat enterohepatic inflammation, oxidative stress and fibrosis due to cholestasis.

Purpose: To investigate the effect of JiaGaSongTang (JGST) and its blood-absorbed ingredient 6-gingerol on α-naphthylisothiocyanate (ANIT)-induced chronic cholestasis, as well as elucidate the underlying regulatory mechanism.

Experimental and Numerical Study of Radioiodine Sorption and Transport in Hanford Sediments.

Radioiodine (I) poses a potential risk to human health and the environment at several U.S. Department of Energy sites, including the Hanford Site, located in southeastern Washington State.

Application of serum Raman spectroscopy combined with classification model for rapid breast cancer screening.

Introduction: This study aimed to evaluate the feasibility of using general Raman spectroscopy as a method to screen for breast cancer. The objective was to develop a machine learning model that utilizes Raman spectroscopy to detect serum samples from breast cancer patients, benign cases, and healthy subjects, with puncture biopsy as the gold standard for comparison. The goal was to explore the value of Raman spectroscopy in the differential diagnosis of breast cancer, benign lesions, and healthy individuals.

Dual-view transport of intensity phase imaging flow cytometry.

In this work, we design multi-parameter phase imaging flow cytometry based on dual-view transport of intensity (MPFC), which integrates phase imaging and microfluidics to a microscope, to obtain single-shot quantitative phase imaging on cells flowing in the microfluidic channel. The MPFC system has been proven with simple configuration, accurate phase retrieval, high imaging contrast, and real-time imaging and has been successfully employed not only in imaging, recognizing, and analyzing the flowing cells even with high-flowing velocities but also in tracking cell motilities, including rotation and binary rotation. Current results suggest that our proposed MPFC provides an effective tool for imaging and analyzing cells in microfluidics and can be potentially used in both fundamental and clinical studies.

A patient with tumor necrosis factor receptor-associated periodic syndrome misdiagnosed as Kawasaki disease: A case report and literature review.

This article reports a case of tumor necrosis factor receptor-associated periodic syndrome (TRAPS) misdiagnosed as Kawasaki disease and summarizes the clinical features and therapeutic progress of TRAPS and the relationship between its clinical manifestations and gene mutations. We retrospectively analyzed a patient with tumor necrosis factor receptor superfamily member 1A (TNFRSF1A) -mutated auto-inflammatory disease who was misdiagnosed with Kawasaki disease in another hospital. The clinical features and therapeutic progress of TRAPS were analyzed by combining clinical features and gene reports of this case and literature review.

Dual-channel binary diffuser-based coherent modulation imaging.

To improve the performance of binary diffuser-based coherent modulation imaging (CMI), a double-channel optical alignment was proposed. Two diffraction patterns formed by the reflection and transmission of a binary diffuser were simultaneously captured and adopted for iterative reconstruction in combination. The information involved in reflected light, not considered in the traditional single-channel optical alignment, was also reconstructed in this dual-channel binary diffuser-based coherent modulation imaging (DB-CMI).

Prognostic value of cell division cycle-associated protein-3 in prostate cancer.

Background: The study was aimed to explore cell division cycle-associated protein-3 (CDCA3) expression and its correlation with clinicopathological characteristics, and identification of co-expressed genes of CDCA3 in prostate cancer (PCa).

Methods: Data for CDCA3 mRNA expression in PCa were obtained from The Cancer Genome Atlas database. Furtherly, CDCA3 protein expression was examined by immunohistochemistry in 80 cases, including 20 normal prostate samples and 60 PCa samples.

Bone marrow mesenchymal stem cells response on collagen/hyaluronan/chondroitin scaffold enriched with gentamicin -loaded gelatin microparticles for skin tissue engineering.

The repair and functional reconstruction of large skin defects caused by burn remains an intractable clinical problem. Collagen type I (ColI) was extracted from carp scales and confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis ultraviolet adsorption spectra and automatic amino acid analyzer. Then the scaffolds containing the purified ColI, hyaluronic acid (HA) and chondroitin sulfate (CS) were constructed and examined.

sPhaseStation: a whole slide quantitative phase imaging system based on dual-view transport of intensity phase microscopy.

Whole slide imaging scans a microscope slide into a high-resolution digital image, and it paves the way from pathology to digital diagnostics. However, most of them rely on bright-field and fluorescence imaging with sample labels. In this work, we designed sPhaseStation, which is a dual-view transport of intensity phase microscopy-based whole slide quantitative phase imaging system for label-free samples.

Phase flow cytometry with coherent modulation imaging.

Label-free imaging and identification of fast-moving cells is a very challenging task. A kind of phase flow cytometry using coherent modulation imaging was proposed to realize label-free imaging and identification on fast-moving cells with compact optical alignment and high accuracy. Phase image of cells under inspection could be computed qualitatively from their diffraction patterns at the accuracy of about 0.

Three-wavelength digital photoelasticity.

A three-wavelength photoelasticity method is proposed to simplify the optical setup and speed up data acquisition. By recording six intensity images with circularly polarized illuminations of three close wavelengths, the phase retardation and corresponding inner stress can be computed accurately with a correspondingly developed computational algorithm. Since the mechanical rotations of wave plates and polarizers required by classic photoelasticity techniques are avoided, the data acquisition of this proposed method is very speedy, and measurement of a dynamic sample can be achieved with a very simple and compact optical setup.

Resolution enhancement with highly curved illumination in ptychography.

By deducing a formula to compute a sample from recorded diffraction intensity directly and analytically, the relationship among the highest reachable resolution of the ptychographic iterative engine (PIE), its illumination angle, and its collection angle was discussed analytically. Curved illumination was then proposed to realize the resolution enhancement for PIE, and a corresponding computing algorithm was proposed to avoid an undersampling effect without increasing the size of the computing matrix, thus realizing speedy high-resolution PIE imaging with a simple experimental setup. While theoretical analysis was carried out, the feasibility of this proposed method was verified both numerically and experimentally.

Single-shot measurement of the near-field and focal spot profiles of a 351 nm laser beam for SGII-upgraded facility with multiple-focal-plane constraint coherent modulation imaging.

Inertial confinement fusion (ICF) places an urgent demand for precise measurement of 351 nm (3ω) laser beam parameters when performing physical experiments on high-power laser facilities. The near-field and focal spot distributions are the utmost important parameters to characterize the quality of the laser beam. Coherent modulation imaging (CMI) is a promising technique for online laser beam measurement, however, it fails to reconstruct the near-field and focal spot profiles when it is used to measure the beam quality of a 351 nm laser beam for SGII-upgrade facility.

Jia-ga-song-tang protection against alcoholic liver and intestinal damage.

Gut-liver axis and cellular homeostasis play key roles in alcohol liver disease (ALD). Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) is a stress-sensitive guarantor of cellular homeostasis. We investigated whether the beneficial effects and underlying mechanisms of Jia-ga-song Tang (JGST) against ALD were associated with gut-liver axis and cellular homeostasis.

Automatic whole blood cell analysis from blood smear using label-free multi-modal imaging with deep neural networks.

Whole blood cell analysis is widely used in medical applications since its results are indicators for diagnosing a series of diseases. In this work, we report automatic whole blood cell analysis from blood smear using label-free multi-modal imaging with deep neural networks. First, a commercial microscope equipped with our developed Phase Real-time Microscope Camera (PhaseRMiC) obtains both bright-field and quantitative phase images.

Research on Rice Yield Prediction Model Based on Deep Learning.

Food is the paramount necessity of the people. With the progress of society and the improvement of social welfare system, the living standards of people all over the world are constantly improving. The development of medical industry improves people's health level constantly, and the world population is constantly climbing to a new peak.

Safety and efficacy of telitacicept in refractory childhood-onset systemic lupus erythematosus: A self-controlled before-after trial.

Objective: To observe the efficacy and safety of telitacicept in refractory childhood-onset systemic lupus erythematosus (cSLE).

Methods: A self-controlled before-after trial. Children with active SLE, aged 5-18 years, who cannot tolerate side effects of glucocorticoid, were enrolled in our study.

Laser-pulse-induced temperature, thermal stress, and crater morphology effect during multipulse nanosecond laser manufacturing.

We construct a numerical model for multipulse laser drilling. It is found that the previous laser-pulse-induced temperature accumulation, thermal stress occurrence, and crater morphology change promote subsequent pulse laser drilling. Among them, previous laser-pulse-induced temperature accumulation contributes significantly to the drilled crater depth when the workpiece temperature is higher than its melting point just before the subsequent laser pulse irradiation, especially in a short pulse interval condition.