Effects of preimplantation genetic testing for aneuploidy on embryo transfer outcomes in women of advanced reproductive age with no more than three retrieved oocytes.

Objective: To evaluate whether preimplantation genetic testing for aneuploidy (PGT-A) benefits women of advanced reproductive age (≥38 years old) with a diminished ovarian reserve (DOR; not more than three retrieved oocytes).

Design: A retrospective analysis comparing two groups: (a) PGT-A group: women who chose PGT-A and subsequent single re-warmed embryo transfers (ETs) and (b) non-PGT-A group: women who chose not to genetically test their embryos and underwent subsequent fresh or re-warmed ETs of one to two embryos on days 3 or 5.

Subjects: Two hundred and thirty patients underwent PGT-A therapy, with 49 of these individuals undergoing more than one PGT-A cycle.

Recyclable NiO/g-CN/ag hybrid substrates for sensitive SERS detection and photo-degradation of residual pesticides in beverages.

In this research, we fabricated an innovative NiO/CN/Ag composite through the straightforward electrostatic assembly of Ag nanoparticles and g-CN sheets onto NiO nanoflowers. This composite enables both surface enhanced Raman spectroscopy (SERS) detection and photo-degradation of pesticides. The results reveal NiO/CN/Ag can quantitatively detect thiram (TRM) and diquat dibromide (DQDB) in water, with a limit of detection (LOD) of 10 M, and also exhibit outstanding photo-degradation efficiency, exceeding 95 % for TRM and DQDB within 90 min under simulated sunlight.

Feasibility of preimplantation genetic testing for aneuploidy on frozen-thawed embryos following conventional IVF insemination.

Objective: Intracytoplasmic sperm injection (ICSI) is commonly employed in preimplantation genetic testing (PGT) to minimize the risk of foreign sperm DNA contamination. Cryopreserved embryos from patients with recurrent miscarriage or repeated implantation failure, who have undergone conventional fertilization (IVF), can be thawed and biopsied for PGT. Therefore, we aimed to assess the accuracy and effectiveness of preimplantation genetic testing for aneuploidy (PGT-A) on frozen embryos using conventional IVF (c-IVF) insemination methods.

The impact of low oocyte maturity ratio on blastocyst euploidy rate: a matched retrospective cohort study.

Objective: To investigate the association between a low oocyte maturity ratio from in vitro fertilization cycle and blastocyst euploidy.

Methods: A total of 563 preimplantation genetic testing (PGT) cycles (PGT cycles with chromosomal structural rearrangements were excluded) were performed between January 2021 and November 2022 at our center (average oocyte maturity rate: 86.4% ± 14.

Construction and optimization of vending machine decision support system based on improved C4.5 decision tree.

The intensification of market competition makes refined operation management become the focus of attention of major manufacturers. As an important branch of artificial intelligence (AI), machine learning (ML) plays a key role in it, and has its application prospect in various systems. Based on this situation, this paper takes vending machines as the research object.

AgTNP@TiO@Ag core-satellite composites for sensitive sensing and in situ monitoring photodegradation of organic dyes by portable Raman spectrometer.

The development of sensitive, reliable, and robust substrates for surface-enhanced Raman spectroscopy (SERS) heavily relies on the creation of numerous hot spots. In this study, we propose a simple approach to fabricate core-satellite composites composed of AgTNP@TiO@Ag, where Ag triangular nanoplates (AgTNPs) act as the cores, TiO serves as the interlayer, and Ag nanoparticles are deposited around them to form Ag satellites. By adjusting the amount of AgNO, we precisely control the coverage of Ag nanoparticles on AgTNP@TiO@Ag, thus fine-tuning their SERS sensitivity.

Establishment of linkage phase, using Oxford Nanopore Technologies, for preimplantation genetic testing of Coffin-Lowry syndrome with a mutation.

This study aimed to perform preimplantation genetic testing (PGT) for a female Coffin-Lowry Syndrome (CLS) patient with a mutation (DNM) in RPS6KA3. It was challenging to establish the haplotype in this family because of the lack of information from affected family members. Hence, we explored a new and reliable strategy for the detection of the DNM in PGT, using Oxford Nanopore Technologies (ONT) and the MARSALA platform.

Multifunctional Ni(OH)/Ag composites for ultrasensitive SERS detection and efficient photocatalytic degradation of ciprofloxacin and methylene blue.

To enable the widespread application of surface-enhanced Raman scattering (SERS) technique in practical sensing of organic pollutants, it is essential to develop a reliable SERS substrate that offers both high sensitivity and reusability. In this study, we employed a simple and rapid in-situ deposition method to coat Ag nanoparticles onto flower-like Ni(OH) spheres, resulting in the formation of Ni(OH)/Ag composites with excellent photocatalytic performance and SERS activity. These composites were used as a promising SERS analysis tool for effective detection of organic pollutants, including ciprofloxacin hydrochloride (CIP) and methylene blue (MB).

Case report: Preimplantation genetic testing for X-linked alport syndrome caused by variation in the gene.

X-Linked Alport Syndrome (XLAS) is an X-linked, dominant, hereditary nephropathy mainly caused by mutations in the gene, found on chromosome Xq22. In this study, we reported a pedigree with XLAS caused by a mutation. This family gave birth to a boy with XLAS who developed hematuria and proteinuria at the age of 1 year.

[Ectopic expression of the AmDREB1F gene from Ammopiptanthus mongolicus enhances stress tolerance of transgenic Arabidopsis].

Dehydration-responsive element binding proteins (DREBs) are an important class of transcription factors related to plant stress tolerance. Ammopiptanthus mongolicus is an evergreen broadleaf shrub endemic to desert areas of northwest China, and it has a very high tolerance to harsh environments. In order to reveal the functions and mechanisms of the AmDREB1F gene from this species in enduring abiotic stresses, we performed subcellular localization test, expression pattern analysis, and stress tolerance evaluation of transgenic Arabidopsis harboring this gene.

An Improved Slice Reconciliation Protocol for Continuous-Variable Quantum Key Distribution.

Reconciliation is an essential procedure for continuous-variable quantum key distribution (CV-QKD). As the most commonly used reconciliation protocol in short-distance CV-QKD, the slice error correction (SEC) allows a system to distill more than 1 bit from each pulse. However, the quantization efficiency is greatly affected by the noisy channel with a low signal-to-noise ratio (SNR), which usually limits the secure distance to about 30 km.

Population-Based Geospatial and Molecular Epidemiologic Study of Tuberculosis Transmission Dynamics, Botswana, 2012-2016.

Tuberculosis (TB) elimination requires interrupting transmission of Mycobacterium tuberculosis. We used a multidisciplinary approach to describe TB transmission in 2 sociodemographically distinct districts in Botswana (Kopanyo Study). During August 2012-March 2016, all patients who had TB were enrolled, their sputum samples were cultured, and M.

A Nested Asymmetric PCR Melting Curve Assay for One-Step Genotyping of Nondeletional α-Thalassemia Mutations.

A rapid DNA-based assay is essential for clinical diagnosis and mass screening in thalassemia-prevention programs. Because of high homology and guanine-cytosine-rich and complex second structure of α-globin genes, it is rather difficult to develop a feasible and simple method for α-thalassemia genotyping. In this study, a strategy of nested asymmetric PCR melting curve analysis was designed to tackle these factors and ensure sensitivity and accuracy.

Effects of cadmium addition on net nitrogen mineralization processes in the urban constructed wetland soils of a Chinese delta.

Heavy metal pollution is a serious problem in wetland ecosystems, and the toxicity of heavy metals affects microorganisms, thus influencing the biogeochemical process of nitrogen (N). To investigate the effects of heavy metal cadmium (Cd) pollution on N mineralization in urban constructed wetland soils of the Pearl River Delta, a 40-day aerobic incubation experiment was conducted under three Cd addition treatments [no Cd addition (control), low Cd addition (LCA) and high Cd addition (HCA)]. The results showed that compared with the control, the LCA treatment enhanced the soil N mineralization rate (R), while the HCA treatment inhibited R, with the average R values in the control treatment of 0.

Microbial resistance and resilience in response to environmental changes under the higher intensity of human activities than global average level.

With the increasing intensity of global human activities, the ecosystem function, which is supported by the microbial community, will be dramatically changed and impaired. To investigate microbial resistance and resilience of microbial communities to human activities, we chose two typical types of human disturbances, urbanization, and reclamation under the higher intensity of human activities than the global average level. We examined microbial traits, including the abundance, diversity, phylogeny, and co-occurrence interactions in soil microbial communities, together with the nitrification activities observed in the subtropical coastal ecosystem of the Pearl River Estuary and in soil microcosm experiments.

Atypical antipsychotics for Parkinson's disease psychosis: a systematic review and meta-analysis.

Purpose: To assess the present evidence regarding the efficiency, safety, and potential risks of pharmacotherapy used for Parkinson's disease psychosis (PDPsy) treatment.

Patients And Methods: We searched the following databases: PubMed, the Cochrane Library, ISI Web of Science, and Embase using the following terms: atypical antipsychotics, pimavanserin, olanzapine, quetiapine, clozapine, Parkinson's disease and psychosis. We systematically reviewed all randomized placebo-controlled trials comparing an atypical antipsychotic with a placebo.

[Genetic and phenotypic analysis of a rare case with homozygous Chinese Gγ (Aγδβ)-thal deletion].

Objective: To analyze the genotype of a patient suspected for thalassemia through a series of experiments.

Methods: Conventional methods for detecting common thalassemia mutations was used in conjunction with multiplex ligation-dependent probe amplification (MLPA) in order to determine the genotype of the patient. Corresponding primers were designed for developing a Gap-PCR system for detecting rare type mutations.

A meta-analysis of effects of selective serotonin reuptake inhibitors on blood pressure in depression treatment: outcomes from placebo and serotonin and noradrenaline reuptake inhibitor controlled trials.

Background: Selective serotonin reuptake inhibitors (SSRIs) and serotonin and noradrenaline reuptake inhibitors (SNRIs) have been commonly prescribed for depression treatment. However, their effects on blood pressure are unclear.

Materials And Methods: Effects on blood pressure of depressive patients in two groups (SSRIs versus placebo and SSRIs versus SNRIs) were evaluated.

Astaxanthin acts via LRP-1 to inhibit inflammation and reverse lipopolysaccharide-induced M1/M2 polarization of microglial cells.

Microglia become activated during neuroinflammation and produce neurotoxic and neurotrophic factors, depending on whether they acquire M1 proinflammatory or M2 anti-inflammatory phenotypes. Astaxanthin (ATX), a natural carotenoid, has anti-inflammatory and neuroprotective effects. We investigated whether ATX could reverse M1/M2 polarization and suppress neuroinflammation low-density lipoprotein receptor-related protein-1 (LRP-1).

Hb H Disease Caused by Multiple Mutations in the Polyadenylation Signal Site and - -/αα.

Thalassemia is the most common monogenic disease, with the highest incidence in Guangxi Zhuang Autonomous Region, People's Republic of China (PRC). The blood test result was not consistent with α-globin gene testing in one of the patients during daily screening. It was confirmed that there were multiple mutations at the α2-globin gene polyadenylation (polyA) signal site: HBA2: c.

N-acetylcysteine ameliorates repetitive/stereotypic behavior due to its antioxidant properties without activation of the canonical Wnt pathway in a valproic acid-induced rat model of autism.

N-acetylcysteine (NAC) is widely used as an antioxidant, and previous studies have suggested that it may have potential as an alternative therapeutic strategy for the treatment of patients with autism. However, the exact effects of NAC administration on the development of autism, as well as the molecular mechanisms underlying its actions, have yet to be fully elucidated. The present study aimed to investigate the effects of NAC on the oxidative status of rats in a valproic acid (VPA)‑induced model of autism, and to examine the involvement of the canonical Wnt signaling pathway in the actions of NAC.

Occurrence, sources, and risk assessment of OCPs in surface sediments from urban, rural, and reclamation-affected rivers of the Pearl River Delta, China.

Sediments were collected to a depth of 20 cm from urban, rural, and reclamation-affected rivers in the Pearl River Delta of China. In total, 16 organochlorine pesticides (OCPs) were analyzed in all sediment samples, and the occurrence, possible sources, toxicity, and health risks of OCPs were evaluated to compare the contamination characteristics of OCPs in sediments among the three types of rivers. The results showed that concentrations of Σ16OCPs in sediments from the three rivers followed the order urban river > reclamation-affected river > rural river, with a mean value of 247.

[Hemoglobin H disease with a rare α-thalassemia gene mutation (--/αα): pedigree analysis and genetic diagnosis].

Objective: To identify a rare α-thalassemia gene mutation in a family from south China and perform a pedigree analysis and genetic diagnosis of hemoglobin H (HbH) disease caused by this mutation.

Methods: Peripheral blood samples were collected from the family members for analysis of the hematological phenotype and routine test of thalassemia genes. DNA sequencing was carried out for samples that showed genotype and phenotype inconsistency.