Publications by authors named "Xiaojun Tian"

Noncoding RNAs (ncRNAs) play critical roles in essential cell fate decisions. However, the exact molecular mechanisms underlying ncRNA-mediated bistable switches remain elusive and controversial. In recent years, systematic mathematical and quantitative experimental analyses have made significant contributions to elucidating the molecular mechanisms of controlling ncRNA-mediated cell fate decision processes.

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Ischemic stroke is a major cause of disability and mortality worldwide, with oxidative stress and blood-brain barrier (BBB) injury playing crucial roles in its pathogenesis. Our RNA sequencing results revealed that S100 calcium-binding protein A4 (S100A4) is highly expressed in the middle cerebral artery occlusion (MCAO) mouse model. We analyzed S100A4 expression in ischemic stroke patients and in mice subjected to the MCAO model.

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Competition among genes for limited transcriptional and translational resources impairs the functionality and modularity of synthetic gene circuits. Traditional control mechanisms, such as feedforward and negative feedback loops, have been proposed to alleviate these challenges, but they often focus on individual modules or inadvertently increase the burden on the system. In this study, we introduce three novel multimodule control strategies─local regulation, global regulation, and negatively competitive regulation (NCR)─that employ an antithetic regulatory mechanism to mitigate resource competition.

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Chiral sensing is essential in biochemistry, biomedicine, and food industries. Traditional chiral sensing mainly focuses on enhancing the chiral near fields, where the quality factor of the resonator has largely been ignored. Here, we propose a whispering gallery mode (WGM) optical microresonator to enhance the chiral signal by exploiting its high quality factor.

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Fluctuations in host cell growth poses a significant challenge to synthetic gene circuits, often disrupting circuit function. Existing solutions typically rely on circuit redesign with alternative topologies or additional control elements, yet a broadly applicable approach remains elusive. Here, we introduce a new strategy based on liquid-liquid phase separation (LLPS) to stabilize circuit performance.

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N6-methyladenosine (mA) is a prevalent mRNA modification known for its implications in various cancer types, yet its role in chromophobe renal cell carcinoma (chRCC) remains largely unexplored. In this study, we performed m6A-SEAL-seq and RNA-seq analyses on tissues from three chRCC subjects, aiming to uncover mA alterations in chRCC. Our findings revealed reduced expression levels of four mA regulators in chRCC tissues and highlighted differences in mA levels compared to normal tissues.

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The nanoscale ampification of light-matter interactions exhibits profound potential in multiple scientific fields, such as physics, chemistry, surface science, materials science, and nanophotonics. Nonetheless, achieving robust optical mode coupling within cavities faces significant hurdles due to modal dispersion and weak optical field confinement. In this theoretical investigation, we demonstrate the viability of strong coupling between the anapole mode of a slotted silicon nanodisk and the plasmonic modes of an Ag nanodisk dimer at visible light frequencies.

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Background: To define the incidence and independent predictive factors of intraoperative adverse events (IOAEs) after minimally invasive radical nephrectomy and thrombectomy (RNAT) and to determine the impact of intraoperative adverse events on oncological outcomes.

Patients And Methods: A total of 294 patients underwent minimally invasive RNAT from January 2010 to December 2023 in our center were included. IOAEs are defined as any deviation from the normal surgical procedure during the operation course.

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Article Synopsis
  • - The study compares the safety and effectiveness of the KangDuo-Surgical Robot-01 (KD-SR-01) and the da Vinci (DV) systems for robot-assisted radical nephroureterectomy (RARNU) through a multicenter trial involving 58 patients from March 2022 to September 2023.
  • - Results showed no significant differences in operative metrics and outcomes between the two systems, with both achieving 100% success rates and no serious complications during a four-week follow-up period.
  • - The KD-SR-01 system is deemed safe and effective for RARNU, although the authors recommend further research with larger sample sizes and extended follow-up for more comprehensive results.
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Objective: To observe the clinical efficacy and safety of the Qingre Lishi decoction in treating of newly diagnosed overweight and obese patients with type 2 diabetes mellitus (T2DM) from an evidence-based medical perspective.

Methods: 70 cases of overweight and obese patients with newly diagnosed T2DM treated in the outpatient clinic of the Department of Endocrinology of the Affiliated Hospital of Liaoning University of Traditional Chinese Medicine from December 2021 to November 2022 were selected, of which 35 cases were in the observation group and 35 cases were in the control group. The observation group was treated with the Qingre Lishi decoction add lifestyle intervention, and the control group was treated with lifestyle intervention only.

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Article Synopsis
  • * A retrospective analysis of 229 patients indicated that those with filled morphology faced more surgical challenges, requiring more techniques and resulting in longer operation times, increased blood loss, and a higher rate of postoperative complications.
  • * The findings suggest that tumor thrombus morphology significantly affects surgical strategies and outcomes, highlighting the need for tailored approaches based on the type of thrombus present.
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Objective: To investigate the postoperative renal function and oncologic outcomes of cystic renal cell carcinoma with partial nephrectomy, and to compared the single-center data on surgical outcomes with the Surveillance, Epidemiology, and End Results (SEER) database.

Methods: This was a retrospective study that included the patients with cystic renal cell carcinoma who underwent partial nephrectomy in the Department of Urology, Peking University Third Hospital (PUTH) from 2010 to 2023. The clinical data and depicting baseline characteristics were collected.

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Article Synopsis
  • The study looked at how to diagnose and treat a rare kidney cancer called renal Ewing's sarcoma, especially when it spreads to the veins.
  • They collected data from 6 patients between 2016 and 2022 to see how well they did after treatment.
  • The results showed that most patients were young females, and those who got chemotherapy lived longer than those who didn't.
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Objective: To analyze the clinicopathological features, prognostic value and surgical treatment experience in patients with adrenocortical carcinoma with venous tumor thrombus.

Methods: We collected relevant data of the patients with adrenocortical carcinoma who had undergone surgery in Peking University Third Hospital from 2018 to 2023. The patients were divided into venous tumor thrombus group and non-tumor thrombus group.

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Background: Tibial cortex transverse transport (TTT) surgery has become an ideal treatment for patients with type 2 severe diabetic foot ulcerations (DFUs) while conventional treatments are ineffective. Based on our clinical practice experience, the protective immune response from TTT surgery may play a role against infections to promote wound healing in patients with DFUs. Therefore, this research aimed to systematically study the specific clinical efficacy and the mechanism of TTT surgery.

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Gene circuits within the same host cell often experience coupling, stemming from the competition for limited resources during transcriptional and translational processes. This resource competition introduces an additional layer of noise to gene expression. Here we present three multi-module antithetic control strategies: negatively competitive regulation (NCR) controller, alongside local and global controllers, aimed at reducing the gene expression noise within the context of resource competition.

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Article Synopsis
  • This study examines renal cell carcinoma (RCC) in young adults aged 18-40, focusing on the differences in outcomes between clear cell RCC (CCRCC) and non-clear cell RCC (NCCRCC) subtypes.
  • Conducted at Peking University Third Hospital from 2012 to 2022, the research analyzed 300 RCC patients who underwent surgery and some received additional therapy to assess clinicopathological features and survival rates.
  • Results indicated that while the 5-year survival rates were high overall, NCCRCC patients had a significantly lower progression-free survival (PFS) compared to CCRCC patients, although overall survival (OS) rates did not significantly differ between the two groups.
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Sepsis is a life-threatening condition that occurs when the body responds to an infection but subsequently triggers widespread inflammation and impaired blood flow. These pathologic responses can rapidly cause multiple organ dysfunction or failure either one by one or simultaneously. The fundamental common mechanisms involved in sepsis-induced multiple organ dysfunction remain unclear.

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  • Researchers have discovered a way to significantly improve upconversion electroluminescence efficiency in single molecules, surpassing previous efforts by over ten times.
  • This enhancement is achieved by carefully engineering the energy levels at the interface between the molecule and the substrate, allowing for a more effective process known as spin-triplet mediated upconversion.
  • The study includes detailed diagrams of electroluminescence which help clarify the conditions necessary for efficient emission, contributing valuable understanding to both single-molecule upconversion and organic electroluminescence as a whole.
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Cells provide dynamic platforms for executing exogenous genetic programs in synthetic biology, resulting in highly context-dependent circuit performance. Recent years have seen an increasing interest in understanding the intricacies of circuit-host relationships, their influence on the synthetic bioengineering workflow, and in devising strategies to alleviate undesired effects. We provide an overview of how emerging circuit-host interactions, such as growth feedback and resource competition, impact both deterministic and stochastic circuit behaviors.

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Background: Surgical treatment for renal cell carcinoma (RCC) and inferior vena cava (IVC) tumor thrombus (TT) is difficult, and the postoperative complication rate is high. This study aimed to explore the safety and oncologic outcomes of neoadjuvant stereotactic ablative body radiotherapy (SABR) combined with surgical treatment for RCC and IVC-TT.

Methods: Patients with RCC and IVC-TTs were enrolled in this study.

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The field of synthetic biology and biosystems engineering increasingly acknowledges the need for a holistic design approach that incorporates circuit-host interactions into the design process. Engineered circuits are not isolated entities but inherently entwined with the dynamic host environment. One such circuit-host interaction, 'growth feedback', results when modifications in host growth patterns influence the operation of gene circuits.

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Background: To define the incidence and risk factors of chyle leak (CL) after radical nephrectomy and thrombectomy and to determine the impact of chyle leak on oncological outcomes.

Patients And Methods: A total of 445 patients who underwent radical nephrectomy and thrombectomy between January 2014 and January 2023 were included. CL is defined as the drainage of chyle with a triglyceride level greater than 110 mg/dL after oral intake or enteral nutrition.

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We report the design and realization of the back focal plane (BFP) imaging for the light emission from a tunnel junction in a low-temperature ultrahigh-vacuum (UHV) scanning tunneling microscope (STM). To achieve the BFP imaging in a UHV environment, a compact "all-in-one" sample holder is designed and fabricated, which allows us to integrate the sample substrate with the photon collection units that include a hemisphere solid immersion lens and an aspherical collecting lens. Such a specially designed holder enables the characterization of light emission both within and beyond the critical angle and also facilitates the optical alignment inside a UHV chamber.

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