Mechanism of Gentisic Acid on Rheumatoid Arthritis Based on miR-19b-3p/RAF1 Axis.

Objective: To investigate the therapeutic effect of gentisic acid (GA) on rheumatoid arthritis (RA) based on the miR-19b-3p/RAF1 axis.

Methods: The cell counting kit-8 method was used to detect the growth inhibitory effect of different concentrations of GA on MH7A cells, and the drug concentration of GA was determined in the experiment. The quantificational real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of miR-19b-3p and RAF1.

The protective effect of gentisic acid on rheumatoid arthritis via the RAF/ERK signaling pathway.

Background: RAF and ERK pathways are known to be activated in human rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS), which play an important role in the pathogenesis and destruction of RA. Gentisic acid (GA) was a natural product derived from plants, which has been reported can attenuate pressure overload-induced cardiac hypertrophy and fibrosis in mice through inhibition of the ERK1/2 pathway. Whether GA can inhibit the occurrence and development of RA through RAF/ERK signaling pathway has not been reported.

Physicochemical and molecular transformation of novel functional peptides from Baijiu.

A novel strategy for screening and identifying peptides present in Baijiu was developed based on magnetic solid-phase extraction with magnetic S-doped graphene (M-G-S) as adsorbent combined with ultrahigh-performance liquid chromatography with high resolution tandem mass spectrometry. In total, 28 peptides consisting of amino acids from 3 to 9 were preliminarily identified, and significantly higher in the number than that of direct concentration and SPE with C as the adsorbent. Six peptides were confirmed with their corresponding synthetic reference standards by comparing their retention time, high resolution MS/MS spectra, and NMR spectroscopic studies.

General recommendation for assessment and management on the risk of glucocorticoid-induced osteonecrosis in patients with COVID-19.

Background/objective: Coronavirus disease 2019 (COVID-19) is a disaster in human medical history and glucocorticoids remain the most promising therapy. Osteonecrosis is a disease caused by reduced intraosseous blood flow to bones in the joints, which will rapidly induce joint destruction. Approximately one-third patients with severe acute respiratory syndrome (SARS) who received high cumulative doses and long treatment durations of glucocorticoids occurred osteonecrosis.

Administration of mircoRNA-135b-reinforced exosomes derived from MSCs ameliorates glucocorticoid-induced osteonecrosis of femoral head (ONFH) in rats.

Exosomes were found to exert a therapeutic effect in the treatment of osteonecrosis of the femoral head (ONFH), while miR-135b was shown to play an important role in the development of ONFH. In this study, we investigated the effects of concomitant administration of exosomes and miR-135b on the treatment of ONFH. A rat mode of ONFH was established.

Effect of on Synovial Cell Apoptosis and TGF-1/Smad Signaling Pathway in Rats with Rheumatoid Arthritis.

Background/aims: (JWFSN) is a new formula originated from , a classic formula for the treatment of rheumatoid arthritis (RA). The mechanism of JWFSN in the treatment of RA is still unclear. The aim of this study was to evaluate the effect of JWFSN formula on the inflammatory mediator levels in the serum and the TGF-1/Smad pathway in the synovium and to explore the underlying mechanisms of JWFSN formula to ameliorate synovial hyperplasia and apoptosis inhibition of synovium in rats with RA.

Multiscale Time-Sharing Elastography Algorithms and Transfer Learning of Clinicopathological Features of Uterine Cervical Cancer for Medical Intelligent Computing System.

Intelligent medical diagnosis and computing system faces many challenges in complex object recognition, large-scale data imaging and real-time diagnosis, such as poor real-time computing, low efficiency of data storage and low recognition rate of lesions. In order to solve the above problems, this paper proposes a medical intelligent computing system and a series of algorithms for the clinical pathology of cervical cancer based on the multi-scale imaging and transfer learning framework. Firstly, based on data dimensions, imaging errors and other factors, this paper designs a multi-scale time-sharing elastic imaging algorithm based on image reconstruction time and data sample characteristics.

The implicit beliefs and implicit behavioral tendencies towards smoking-related cues among Chinese male smokers and non-smokers.

Background: The dual-process theory is central to several models of addiction, implying the importance of automatic processes in the maintenance and development of addiction. Implicit beliefs are traces of previous experience which relate to the representation in cognition. Implicit behavioral tendencies are traces of previous experience which relate to the representation in behavioral tendencies.

Corrigendum: Expanded CURB-65: a new score system predicts severity of community-acquired pneumonia with superior efficiency.

This corrects the article DOI: 10.1038/srep22911.

Immature monocytes contribute to cardiopulmonary bypass-induced acute lung injury by generating inflammatory descendants.

Background: As immune regulatory and effector cells, monocytes play an important role in the blood-extracorporeal circuit contact-related acute lung injury in patients undergoing cardiopulmonary bypass (CPB). However, circulating monocytes are phenotypically and functionally heterogeneous, so we characterised how immature monocytes affect acute lung injury induced by CPB.

Methods: The identification and dynamic changes in monocyte subsets were monitored by flow cytometry in patients undergoing CPB and in a rat model of CPB.

Expanded CURB-65: a new score system predicts severity of community-acquired pneumonia with superior efficiency.

Aim of this study was to develop a new simpler and more effective severity score for community-acquired pneumonia (CAP) patients. A total of 1640 consecutive hospitalized CAP patients in Second Affiliated Hospital of Zhejiang University were included. The effectiveness of different pneumonia severity scores to predict mortality was compared, and the performance of the new score was validated on an external cohort of 1164 patients with pneumonia admitted to a teaching hospital in Italy.

[Roof folding and rotary pushing for the treatment of back to back fractures of distal radius and ulna in children].

Objective: To evaluate the technique and the clinical effect of folding roof and rotary pushing in treatment of children with distal radius and ulna fracture of "back to back".

Methods: From January 2012 to February 2014,38 children with distal radius and ulna fracture of "back to back" were treated by using the technique of folding roof and rotary pushing to reset and splint fixation including 23 males and 15 females with an average age of 9.5 years old ranging from 6 to 14 years old.

Imbalance between circulating CD4+ regulatory T and conventional T lymphocytes in patients with HBV-related acute-on-chronic liver failure.

Background & Aims: The important pathophysiological role of immune dysfunction, especially innate immune dysfunction in patients with acute-on-chronic liver failure (ACLF), has been investigated in recent years, but dysregulation of adaptive immunity remains poorly elucidated. The aim of this study was to (i) determine the CD3(+) T-lymphocyte count and the balance between CD4(+) regulatory T (Tregs) and conventional T cells (Tconv) in hepatitis B virus (HBV)-related ACLF patients; (ii) analyse the frequencies of Tregs subpopulations; and (iii) assess the suppressive potency of CD4(+) Tregs and each fraction.

Methods: We enrolled 20 HBV-ACLF patients, 10 septic shock subjects, 20 chronic hepatitis B (CHB) patients and 20 healthy volunteers (HC).

[Characterization of a p-nitrophenol degrading bacterium Pseudomonas sp. PDS-7 and cloning of degradation relevant genes].

Objective: The aim of this study was to (i) isolate and characterize bacteria capable of degrading p-nitrophenol (PNP); (ii) determine the kinetics of biodegradation, (iii) clone and express the PNP-degrading related genes.

Methods: Enrichment method and serial dilution spread-plate method were employed to isolate PNP-degrading strain. Morphological, physiological & biochemical tests and 16S rDNA sequence analysis were used to identify the isolate.

[Isolation of phenol-degrading bacteria from natural soil and their phylogenetic analysis].

Five bacterial strains capable of utilizing phenol as sole carbon source for growth were isolated from non-contaminated natural soil sample after enrichment in the presence of phenol. They were preliminarily identified according to their phylogenetic analysis and physiological and biochemical characteristics. Strain PHD-2, PHD-4 and PHD-5 belonged to the genera of Ralstonia, Acinetobacter and Microbacterium respectively; strain PHD-1 and PHD-3 were from the genus of Pseudomonas.

Isolation and characterization of a carbofuran-degrading strain Novosphingobium sp. FND-3.

A gram-negative Novosphingobium sp. strain FND-3 capable of degrading carbofuran was isolated and characterized. The carbofuran-degrading ability of strain FND-3 was investigated under various culture conditions.

Suppression of IL-8 gene transcription by resveratrol in phorbol ester treated human monocytic cells.

Resveratrol (3,4',5-trihydroxy-trans-silbene), a natural phytoalexin found in grapes and other food products, has promising anti-inflammatory and anticancer effects. To observe the modulation of interleukin-8 (IL-8) production in human monocytic cells by resveratrol and explore its mechanism at the gene transcription level, U937 cells were stimulated with phorbol 12-myristate 13-acetate (PMA) for 24h. IL-8 protein in supernatants was measured by radioimmunoassay.